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Analyses of cross-talk between different photoreceptors in plant light signal transduction

植物光信号转导中不同光感受器之间的串扰分析

基本信息

批准号：
15370020
负责人：
NAGATANI Akira
金额：
$ 9.79万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Morphology of plants depends very much on the light environment. In those responses, which are often referred to as photomorphogenesis, a red/far-red light photoreceptor, phytochrome, and a blue light photoreceptor, cryptochrome play a key roles. In this year, we investigated how the signal from these photoreceptors are integrated to coordinately regulate the plant's morphogenesis. A brief summary of the achievement is as follows.1)The PHY domain consisting of about 200 amino acid residues is recognized as a sub-domain of phytochrome within the photosensory domain of phytochrome (about 650 amino acid residues). We examined the signaling activity of the N-terminal domain lacking this PHY sub-domain. The results demonstrated that the PHY domain was dispensable for the signaling but was required to keep the active form of phytochrome stable. In addition, we identified many missense mutants within the N-terminal photosensory domain of phyB. Analysis of these mutations suggested different roles for different sub-domains of phytochrome. 2)We examine the involvement of phytochromes and cryptochrimes in the light-induced root greening. Consequently, we found that both the photoreceptors needed to be activated to case the full root greening. We are currently testing whether or not fragments of phyB have similar activity. 3)We screened for mutants in a T-DNA-based activation-tagged pool of Arabidopsis and identified a causal gene for one of such mutants. The results revealed that over-expression of the CYP72C1 gene reduced the level of active brassinosteroids, which led to partial photomorphogenesis in darkness.

植物的形态在很大程度上取决于光环境。在这些反应中，通常被称为光形态建成，红光/远红光感光器，光敏色素和蓝光感光器，隐花色素发挥关键作用。今年，我们研究了这些光感受器的信号是如何整合在一起协调调节植物形态建成的。1）在光敏色素光敏结构域（约650个氨基酸残基）中，含有约200个氨基酸残基的PHY结构域被认为是光敏色素的亚结构域。我们检查了缺乏该PHY子域的N-末端结构域的信号传导活性。结果表明，PHY结构域对于信号传导是不可或缺的，但对于保持光敏色素活性形式的稳定是必需的。此外，我们确定了许多错义突变体内的N-末端光敏结构域的phyB。对这些突变的分析表明光敏色素的不同亚结构域具有不同的作用。2）我们研究了光敏色素和隐花色素在光诱导的根绿化中的参与。因此，我们发现，这两种光感受器都需要被激活，以实现完全的根绿化。我们目前正在测试phyB的片段是否具有类似的活性。3）我们在拟南芥的基于T-DNA的激活标记库中筛选突变体，并鉴定了一个此类突变体的致病基因。结果表明，CYP 72 C1基因的过表达降低了活性油菜素类固醇的水平，导致黑暗中的部分光形态建成。

项目成果

期刊论文数量（76）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

光受容体と光応答

光感受器和光反应

DOI：
发表时间：
2004
期刊：
植物の環境応答と形態形成のクロストーク(岡宏, 岡田清孝, 篠崎一雄編)
影响因子：
0
作者：
田中慎一郎;長谷あきら
通讯作者：
長谷あきら

Photoreceptors and photoresponses

光感受器和光反应

DOI：
发表时间：
2004
期刊：
In "Cross talk between environmental responses and morphogenesis in plants" edited by A.Oka, K.Okada and K.Shinozaki (in Japanese)
影响因子：
0
作者：
TANAKA S-I;A.NAGATANI
通讯作者：
A.NAGATANI

Dimers of the N-terminal domain of phytochrome B are functional in the nucleus

DOI：
10.1038/nature01837
发表时间：
2003-07-31
期刊：
NATURE
影响因子：
64.8
作者：
Matsushita, T;Mochizuki, N;Nagatani, A
通讯作者：
Nagatani, A

Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis.

DOI：
10.1093/pcp/pcg148
发表时间：
2003-11
期刊：
Plant & cell physiology
影响因子：
4.9
作者：
Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N
通讯作者：
Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N

Activation of the cytochrome P450 gene, CYP72C1, reduces the levels of active brassinosteroids in vivo