Analyses of cross-talk between different photoreceptors in plant light signal transduction

植物光信号转导中不同光感受器之间的串扰分析

基本信息

批准号：
15370020
负责人：
NAGATANI Akira
金额：
$ 9.79万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Morphology of plants depends very much on the light environment. In those responses, which are often referred to as photomorphogenesis, a red/far-red light photoreceptor, phytochrome, and a blue light photoreceptor, cryptochrome play a key roles. In this year, we investigated how the signal from these photoreceptors are integrated to coordinately regulate the plant's morphogenesis. A brief summary of the achievement is as follows.1)The PHY domain consisting of about 200 amino acid residues is recognized as a sub-domain of phytochrome within the photosensory domain of phytochrome (about 650 amino acid residues). We examined the signaling activity of the N-terminal domain lacking this PHY sub-domain. The results demonstrated that the PHY domain was dispensable for the signaling but was required to keep the active form of phytochrome stable. In addition, we identified many missense mutants within the N-terminal photosensory domain of phyB. Analysis of these mutations suggested different roles for different sub-domains of phytochrome. 2)We examine the involvement of phytochromes and cryptochrimes in the light-induced root greening. Consequently, we found that both the photoreceptors needed to be activated to case the full root greening. We are currently testing whether or not fragments of phyB have similar activity. 3)We screened for mutants in a T-DNA-based activation-tagged pool of Arabidopsis and identified a causal gene for one of such mutants. The results revealed that over-expression of the CYP72C1 gene reduced the level of active brassinosteroids, which led to partial photomorphogenesis in darkness.

植物的形态在很大程度上取决于光环境。在这些反应中，通常称为光型生成，红色/远红色光感受器，植物色素和蓝光光感受器，加密色素扮演着关键角色。在今年，我们研究了这些感光体的信号如何集成以协调调节植物的形态发生。该成就的简要摘要如下。1）由大约200个氨基酸残基组成的PHY结构域被认为是植物色素光（大约650个氨基酸残基）中植物色素的子域。我们检查了缺乏该PHY子域的N末端结构域的信号传导活性。结果表明，PHY结构域对于信号传导是可分配的，但需要保持植物色素稳定的活性形式。此外，我们确定了PHYB N末端光感域内的许多错义突变体。对这些突变的分析表明，植物色素的不同亚构造的作用不同。 2）我们检查了植物色素和加密摄影的参与。因此，我们发现，两个光感受器都需要激活为完整的根绿色。我们目前正在测试Phyb片段是否具有相似的活性。 3）我们在基于T-DNA的激活标签的拟南芥中筛选了突变体，并确定了其中一个突变体的因果基因。结果表明，CYP72C1基因的过表达降低了活性的腕足激素的水平，从而导致黑暗中的部分光晶生成。

项目成果

期刊论文数量（76）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Photoreceptors and photoresponses

光感受器和光反应

DOI：
发表时间：
2004
期刊：
In "Cross talk between environmental responses and morphogenesis in plants" edited by A.Oka, K.Okada and K.Shinozaki (in Japanese)
影响因子：
0
作者：
TANAKA S-I;A.NAGATANI
通讯作者：
A.NAGATANI

光受容体と光応答

光感受器和光反应

DOI：
发表时间：
2004
期刊：
植物の環境応答と形態形成のクロストーク(岡宏, 岡田清孝, 篠崎一雄編)
影响因子：
0
作者：
田中慎一郎;長谷あきら
通讯作者：
長谷あきら

Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis.

DOI：
10.1093/pcp/pcg148
发表时间：
2003-11
期刊：
Plant & cell physiology
影响因子：
4.9
作者：
Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N
通讯作者：
Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N

Light-regulated nuclear localization phytochromes.

光调节核定位光敏色素。