Analyses of cross-talk between different photoreceptors in plant light signal transduction
植物光信号转导中不同光感受器之间的串扰分析
基本信息
- 批准号:15370020
- 负责人:
- 金额:$ 9.79万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Morphology of plants depends very much on the light environment. In those responses, which are often referred to as photomorphogenesis, a red/far-red light photoreceptor, phytochrome, and a blue light photoreceptor, cryptochrome play a key roles. In this year, we investigated how the signal from these photoreceptors are integrated to coordinately regulate the plant's morphogenesis. A brief summary of the achievement is as follows.1)The PHY domain consisting of about 200 amino acid residues is recognized as a sub-domain of phytochrome within the photosensory domain of phytochrome (about 650 amino acid residues). We examined the signaling activity of the N-terminal domain lacking this PHY sub-domain. The results demonstrated that the PHY domain was dispensable for the signaling but was required to keep the active form of phytochrome stable. In addition, we identified many missense mutants within the N-terminal photosensory domain of phyB. Analysis of these mutations suggested different roles for different sub-domains of phytochrome. 2)We examine the involvement of phytochromes and cryptochrimes in the light-induced root greening. Consequently, we found that both the photoreceptors needed to be activated to case the full root greening. We are currently testing whether or not fragments of phyB have similar activity. 3)We screened for mutants in a T-DNA-based activation-tagged pool of Arabidopsis and identified a causal gene for one of such mutants. The results revealed that over-expression of the CYP72C1 gene reduced the level of active brassinosteroids, which led to partial photomorphogenesis in darkness.
植物的形态在很大程度上取决于光环境。在这些反应中,通常被称为光形态建成、红/远红光感受器、光敏色素和蓝光感受器,隐色素发挥着关键作用。在这一年里,我们研究了来自这些光感受器的信号是如何整合来协调调节植物的形态发生的。1)由约200个氨基酸残基组成的PHY结构域被认为是光敏色素光敏结构域(约650个氨基酸残基)中的一个光敏色素亚域。我们检测了缺少这个PHY亚结构域的N-末端结构域的信号活性。结果表明,PHY结构域对于信号转导是必要的,但需要保持光敏色素活性形式的稳定。此外,我们还在PhyB的N端感光结构域中发现了许多错义突变体。对这些突变的分析表明,光敏色素的不同亚域具有不同的作用。2)研究了光敏色素和隐配子体在光诱导的根绿化过程中的作用。因此,我们发现这两个光感受器都需要被激活才能使根完全绿化。我们目前正在测试PhyB的片段是否具有类似的活性。3)我们在基于T-DNA的激活标签库中筛选了突变体,并确定了其中一个突变体的致病基因。结果表明,细胞色素P72C1基因的过度表达降低了油菜素类化合物的活性水平,从而导致了黑暗中的部分光形态建成。
项目成果
期刊论文数量(76)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Photoreceptors and photoresponses
光感受器和光反应
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:TANAKA S-I;A.NAGATANI
- 通讯作者:A.NAGATANI
Dimers of the N-terminal domain of phytochrome B are functional in the nucleus
- DOI:10.1038/nature01837
- 发表时间:2003-07-31
- 期刊:
- 影响因子:64.8
- 作者:Matsushita, T;Mochizuki, N;Nagatani, A
- 通讯作者:Nagatani, A
Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis.
- DOI:10.1093/pcp/pcg148
- 发表时间:2003-11
- 期刊:
- 影响因子:4.9
- 作者:Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N
- 通讯作者:Yoko Yamamoto;Eriko Sato;Tomo Shimizu;N. Nakamich;Shusei Sato;Tomohiko Kato;S. Tabata;A. Nagatani-A.-N
Activation of the cytochrome P450 gene, CYP72C1, reduces the levels of active brassinosteroids in vivo
- DOI:10.1093/jxb/eri073
- 发表时间:2005-03-01
- 期刊:
- 影响因子:6.9
- 作者:Nakamura, M;Satoh, T;Nagatani, A
- 通讯作者:Nagatani, A
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NAGATANI Akira其他文献
NAGATANI Akira的其他文献
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{{ truncateString('NAGATANI Akira', 18)}}的其他基金
Spatial structure and mechanisms of plant responses to light
植物对光反应的空间结构和机制
- 批准号:
15H04389 - 财政年份:2015
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of the mechanism for plant responses to external force by a combination of plant physiological and physical approaches
结合植物生理学和物理方法阐明植物对外力反应的机制
- 批准号:
15K14544 - 财政年份:2015
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Elucidation of molecular mechanisms underlying hyper sensitization of phytochrome A
阐明光敏色素 A 超敏化的分子机制
- 批准号:
21370020 - 财政年份:2009
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of the molecular mechansim for movement regulation by phototropin
阐明向光素运动调节的分子机制
- 批准号:
17084002 - 财政年份:2005
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Signal transduction mechanism of phytochrome
光敏色素的信号转导机制
- 批准号:
17370018 - 财政年份:2005
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of light-regulated hypocotyl elongation
光调节下胚轴伸长的分子机制
- 批准号:
13440239 - 财政年份:2001
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Moleculargenetical analysis of phytochrome siggnal transduction
光敏色素信号转导的分子遗传学分析
- 批准号:
08454253 - 财政年份:1996
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Moleculaabiological analysis of phytochrome siggnal transduction
光敏色素信号转导的分子生物学分析
- 批准号:
06454018 - 财政年份:1994
- 资助金额:
$ 9.79万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
PHYTOCHROME SIGNAL TRANSDUCTION - BLUE ABSORBING CYANOBACTERIAL PHYTOCHROMES
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8168966 - 财政年份:2010
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- 资助金额:
$ 9.79万 - 项目类别:
PHYTOCHROME SIGNAL TRANSDUCTION - BLUE ABSORBING CYANOBACTERIAL PHYTOCHROMES
光敏色素信号转导 - 蓝色吸收蓝藻光敏色素
- 批准号:
7954671 - 财政年份:2009
- 资助金额:
$ 9.79万 - 项目类别:
PHYTOCHROME SIGNAL TRANSDUCTION - THERMOSTABLE CYANOBACTERIAL PHYTOCHROMES
光敏色素信号转导 - 耐热蓝细菌光敏色素
- 批准号:
7721628 - 财政年份:2008
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- 批准号:
7598734 - 财政年份:2007
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$ 9.79万 - 项目类别:
Signal transduction mechanism of phytochrome
光敏色素的信号转导机制
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17370018 - 财政年份:2005
- 资助金额:
$ 9.79万 - 项目类别:
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Arabidopsis phytochrome A signal transduction: characterization and identifcation of cytoplasmic events and components
拟南芥光敏色素 A 信号转导:细胞质事件和成分的表征和鉴定
- 批准号:
15990434 - 财政年份:2005
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Linear tetrapyrroles and phytochrome-like sensors in signal transduction of the opportunistic pathogen Pseudomonas aeruginosa
线性四吡咯和光敏色素样传感器在机会性病原体铜绿假单胞菌信号转导中的作用
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5385900 - 财政年份:2002
- 资助金额:
$ 9.79万 - 项目类别:
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