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Molecular analysis of plant Na and K transporter involved in response to environmental stress

参与环境胁迫响应的植物钠和钾转运蛋白的分子分析

基本信息

批准号：
15380070
负责人：
UOZUMI Nobuyuki
金额：
$ 6.53万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380070/
关键词：
Na transporter K transporter K channel AtHKT1 HKT Ktr Arabidopsis cyanobacteria EF-hand K^+らん藻 KUP スフェロプラスト大腸菌プロトン駆動力

项目摘要

1 The Ktr-system in Synechocystis sp.PCC 6803 was found to be Na-activated K transporter. The K transport activity was inhibited by CCCP and was not found in the mutant strain which has a mutation of the respiratory chain system. These indicate that proton motive force is involved in the Ktr-mediated K transport. Hyperosmotic stress led initially to loss of net K from the cells. The ktrB mutant shocked with sorbitol failed to reaccumulate K up to its original level. The K uptake via KtrABE palys a crucial role in the early phase of cell turgor regulation after hyperosmotic shock.2 Bacterial Ktr system and plant HKT-system has eight transmembrane topology. The positive residue in the eighth transmembrane segment was replaced with other amino acids. The conversion from Arg to Lys sustained the K uptake activity but that to negative or non-polar amino acids gave loss of the function. The positive residue is important for the activity.3 KUP-type transporter was not inserted into the rough microsomal membrane. Then the transporter was expressed in E.coli and their membrane structure was determined.4 Some of plant K channels has the putative Ca binding sites. The mutation in the region inhibited the K uptake activity. The region is important for the transport activity.5 Arabidopsis AtHKT1 transporter was expressed in the vascular tissuels in the aerial part of the plant. The null mutant showed that Na accumulated in the xylem tissue and Na was not supplied into the phloem tissue. AtHKT1 is one of the components for Na recirculation of plant.

1 .聚囊藻（Synechocystis sp.PCC 6803）的ktr -系统是na激活的K转运体。K转运活性被CCCP抑制，而在呼吸链系统突变的突变株中没有发现。这表明质子动力参与了ktr介导的K转运。高渗应激最初导致细胞中净钾的损失。山梨糖醇刺激的ktrB突变体未能将K重新积累到原来的水平。通过KtrABE的钾摄取在高渗休克后的细胞肿胀调节的早期阶段起着至关重要的作用细菌的Ktr系统和植物的hkt系统具有八种跨膜拓扑结构。第八跨膜段的正残基被其他氨基酸取代。精氨酸向赖氨酸的转化维持了钾吸收活性，而向负极性或非极性氨基酸的转化则丧失了钾吸收功能。正残留对活性很重要kup型转运蛋白未插入粗粒体膜。然后在大肠杆菌中表达该转运蛋白，并测定其膜结构一些植物的钾离子通道具有假定的钙离子结合位点。该区域的突变抑制了钾吸收活性。这个地区对运输活动很重要AtHKT1转运蛋白在拟南芥地上部维管组织中表达。零突变体表明，钠在木质部组织中积累，而不向韧皮部组织供应。AtHKT1是植物Na再循环的组分之一。

项目成果

期刊论文数量（46）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Umigai, N.et al.: "Topogenesis of two transmembrane type K^+ channels, Kir2.1 and KcsA"J.Biol.Chem.. 278. 40373-40384 (2003)

Umigai, N.等人：“两种跨膜类型 K^ 通道的拓扑发生，Kir2.1 和 KcsA”J.Biol.Chem.. 278. 40373-40384 (2003)

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

植物の代謝コミュニケーション植物分子生理学の新展開蛋白質核酸酵素増刊号48

植物代谢通讯植物分子生理学新进展蛋白质核酸酶特刊第48期

DOI：
发表时间：
2003
期刊：
影响因子：
0
作者：
佐藤陽子;水谷昭文;魚住信之
通讯作者：
魚住信之

Recent progress of biochemical and biomedical engineering in Japan.

日本生物化学和生物医学工程的最新进展。

DOI：
发表时间：
2004
期刊：
Recent progress of biochemical and biomedical engineering in Japan II.
影响因子：
0
作者：
^*Tateishi Y;^*Kawabe Y(^*co-first author);Chiba T;Murata S;Ichikawa K;Murayama A;Tanaka K;Baba T;Kato S;Yanagisawa J.;Uozumi N.
通讯作者：
Uozumi N.

Metabolic communication in plant : Development of plant molecular physiology

植物代谢通讯：植物分子生理学的发展