Elucidation of the Na and K transport system conferring salinity tolerance in plant cells

阐明赋予植物细胞耐盐性的钠和钾运输系统

• 批准号: 22380056
• 负责人: UOZUMI Nobuyuki
• 金额: $ 9.65万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380056/
• 关键词: 輸送体; カリウム; ナトリウム; 遺伝子発現; 膜タンパク質; 耐塩性; 浸透圧; AtHKT1; K輸送体; シロイヌナズナ; イオンバランス; AtNHX1; プロモーター; Na循環系; 概日性リズム発現; ルシフェラーゼ遺伝子; Na輸送体; AtKUP1; AtHB

Drought stress and accumulation of salt lead to increase the osmolarity of the environmental space. This gave the dehydration of plant cells, which ends up to jeopardize the plant life. The water movement across the biological membrane is affected by osmolarity and therefore the concentrations of cation, sodium ion and potassium ion are crucial for them. The intracellular concentrations of these cation play an important role in ion homeostasis in plant cells. In this study, membrane transport system for sodium ion and potassium ion has been analyzed and their structure and function has been investigated. The increase of the resistance against the environmental stress in plant cells has been studied through the elucidation of their sodium and potassium transport system, which is closely involved in understanding the molecular mechanism of osmolarity homeostasis.

干旱胁迫和盐的积累导致环境空间渗透压增加。这导致植物细胞脱水，最终危及植物的生命。水在生物膜上的运动受到渗透压的影响，因此阳离子、钠离子和钾离子的浓度对它们至关重要。这些阳离子的胞内浓度在植物细胞离子稳态中起着重要作用。本研究分析了钠离子和钾离子的膜转运系统，并对其结构和功能进行了研究。通过阐明植物细胞的钠、钾转运系统，研究了植物细胞对环境胁迫抵抗力的增强，这与了解渗透稳态的分子机制密切相关。

项目成果

植物K流入系チャネルAKT1のリン酸化活性制御

植物钾流入通道 AKT1 磷酸化活性的调节

• 发表时间: 2012
• 作者: Hattori H;Yakushi T;Matsutani M;Moonmangmee D;Toyama H;Adachi O;Matsushita K;浜本晋,中山浩太,松本尚樹,佐藤裕樹,七谷圭,魚住信之
• 通讯作者: 浜本晋,中山浩太,松本尚樹,佐藤裕樹,七谷圭,魚住信之

巨大化酵母パッチクランプ法を用いた酵母陽イオンチャネルYVC1の輸送特性

使用巨型酵母膜片钳方法研究酵母阳离子通道YVC1的传输特性

• 发表时间: 2010
• 作者: 浜本晋;森泰生;矢部勇;魚住信之
• 通讯作者: 魚住信之

KAT1 is differentially phosphorylated by SnRK2.6 and calcium-dependent kinase, which reduces its transport activity.

KAT1 被 SnRK2.6 和钙依赖性激酶差异磷酸化，从而降低其转运活性。

• 发表时间: 2010
• 作者: Uozumi N;Sato A;Sato Y;Fukao Y;Fujiwara M;Umezawa T;Shinozaki K;Hibi T;Taniguchi M;Miyake M;Dreyer I;Gambale F;Goto D.
• 通讯作者: Goto D.

Characterization of YVC1 Channel in Yeast Vacuolar Membrane

酵母液泡膜中 YVC1 通道的表征

• 发表时间: 2012
• 作者: Hamamoto;S.;Mori;Y.;Yabe;I.;Uozumi;N
• 通讯作者: N

Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting.

• DOI: 10.1074/jbc.m111.252650
• 发表时间: 2011-09-30
• 期刊: The Journal of biological chemistry
• 作者: Chanroj S;Lu Y;Padmanaban S;Nanatani K;Uozumi N;Rao R;Sze H
• 通讯作者: Sze H