Molecular analysis of ion transporters in plant and cyanobacteria involved in response to high osmolality
植物和蓝藻中参与高渗透压反应的离子转运蛋白的分子分析
基本信息
- 批准号:17380064
- 负责人:
- 金额:$ 6.46万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. We have identified the localization of Arabidopsis Na transporter AtHKT1 in plant cells. The specificity of the anti-AtHKT1 antibodies was confirmed. Immunoelectron microscopy using the antibodies demonstrate that AtHKT1 is targeted to the plasma membrane in xylem parenchyma cells in leaves.2. AtHKT1 disruption alleles caused large increases in the Na content of the xylem sap and conversely reduced the Na content of the phloem sap. The athkt1 mutant alleles had a smaller and inverse influence on the K content compared with the Na content to the xylem, suggesting that K transport may be indirectly affected. The expression of AtHKT1 was modulated not only by the concentrations of Na and K but also by the osmolality of non-ionic compounds.3. The conserved Arg near the middle of the M2D segment was essential for the K^+ transport of KtrB. We propose the existence of the salt bridge(s) between the positive residues in the M2D and the conserved negative residues in the pore region, which reduces an electrostatic barrier against cation permeation caused by the positive residue(s) and that stabilizes the transporter configuration.4. Mutation of a conserved His-157 in the second pore loop of KtrB, drastically reduced the activity of the K^+ transporter KtrABE system from Synechocystis PCC 6803. The result suggests that His-157 plays an essential role in the K^+ transport activity of the transporter system.5. H transport activity of one of Synechocystis Na/H transporters was measured using the inverted membrane vesicle of E. coli expressing the protein. We have identified the charged residues essential for the K transport.
1.我们已经确定了拟南芥钠转运蛋白AtHKT 1在植物细胞中的定位。证实了抗AtHKT 1抗体的特异性。免疫电镜显示AtHKT 1定位于叶片木质部薄壁细胞的质膜上. AtHKT 1破坏等位基因引起木质部汁液的Na含量的大幅度增加,相反地降低了韧皮部汁液的Na含量。athkt 1突变体等位基因有一个较小的和相反的影响K含量相比,Na含量的木质部,这表明K运输可能会间接影响。AtHKT 1的表达不仅受Na和K浓度的调节,还受非离子化合物渗透压的调节. M2 D片段中部附近的保守Arg对KtrB的K^+转运至关重要。我们提出在M2 D中的正性残基和孔区域中保守的负性残基之间存在盐桥,这降低了由正性残基引起的对阳离子渗透的静电屏障,并且稳定了转运体构型. KtrB第二孔环中保守His-157的突变大大降低了集胞藻PCC 6803的K^+转运蛋白KtrABE系统的活性。结果表明His-157在转运系统的K^+转运活性中起着重要作用.利用E.大肠杆菌表达该蛋白。我们已经确定了K运输所必需的带电残基。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
膜電位依存性Kチャネル(イオンチャネル 最前線update)別冊・医学のあゆみ(倉智嘉久監修)
膜电位门控 K 通道(离子通道前线更新)单独卷/病史(由 Yoshihisa Kurachi 监督)
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tholema;N.;Iida et al.;Sunarpi et al.;Tholema et al.;Matsuda et al.;魚住信之;魚住信之;魚住信之;魚住信之
- 通讯作者:魚住信之
Ktr-mediated potassium transport, a major pathway for potassium uptake, is coupled to a proton gradient across the membrane in the Synechocystis sp.PCC6803.
Ktr 介导的钾转运是钾吸收的主要途径,在集胞藻 PCC6803 中与跨膜质子梯度耦合。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Matsuda;N.;Uozumi;N.
- 通讯作者:N.
Mutation of His-157 in the second pore loop drastically reduces the activity of Synechocystis Ktr transporter.
第二个孔环中 His-157 的突变大大降低了集胞藻 Ktr 转运蛋白的活性。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Zulkifli;L.;Uozumi;N.
- 通讯作者:N.
Further application of a two-step heparin affinity chromatography method using divalent cation as eluents : Purification and identification of membrane-bound heparin binding proteins from the mitochondrial fraction of HL-60 cells.
使用二价阳离子作为洗脱液的两步肝素亲和层析方法的进一步应用:从 HL-60 细胞的线粒体部分中纯化和鉴定膜结合的肝素结合蛋白。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tholema;N.;Iida et al.
- 通讯作者:Iida et al.
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UOZUMI Nobuyuki其他文献
UOZUMI Nobuyuki的其他文献
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{{ truncateString('UOZUMI Nobuyuki', 18)}}的其他基金
Elucidation of the Na and K transport system conferring salinity tolerance in plant cells
阐明赋予植物细胞耐盐性的钠和钾运输系统
- 批准号:
22380056 - 财政年份:2010
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of function of ion channel using giant bacteria and identification of membrane transporters
利用巨型细菌分析离子通道的功能及膜转运蛋白的鉴定
- 批准号:
19380058 - 财政年份:2007
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on plant Na and K transport system, which is involved in the formation of membrane potential and adaptation of osmolality
植物钠钾转运系统的研究,涉及膜电位的形成和渗透压的适应
- 批准号:
17078005 - 财政年份:2005
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular analysis of plant Na and K transporter involved in response to environmental stress
参与环境胁迫响应的植物钠和钾转运蛋白的分子分析
- 批准号:
15380070 - 财政年份:2003
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Physiological function of Na^+/K^+ transporters from bacteria and plants
细菌和植物Na^/K^转运蛋白的生理功能
- 批准号:
13660088 - 财政年份:2001
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on function of the diverse K^+ transporters from plants
植物多种K^转运蛋白的功能研究
- 批准号:
11660082 - 财政年份:1999
- 资助金额:
$ 6.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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