Physiological function of Na^+/K^+ transporters from bacteria and plants
Physiological function of Na^+/K^+ transporters from bacteria and plants
批准号:
13660088
负责人:
UOZUMI Nobuyuki
金额:
$2.3万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002
中文摘要
1. 来自拟南芥的AtHKTl (AtHKTl)形成4层膜-孔-膜结构。发现HKT转运体的p环仅在介导K^+选择性的甘氨酸残基之一中表现出同一性。HKT转运蛋白有一个共同的祖先转运蛋白,具有4个环。KATl通道与其他动植物的K^+通道具有典型的相似性。KATl中S3和S4的电压感应片段只有在它们之间发生特异性相互作用时才会在翻译后整合到膜中。插入过程是新近发现的第二种膜蛋白整合过程。细菌Na^+/H^+反转运蛋白跨膜中的天冬氨酸参与H^+的渗透,但不参与Na^+的渗透。AtHKTl在植物维管组织中表达。AtHKTl在植物体内Na^+的再循环中起作用。测定了胞囊菌PCC6803中hkt型转运体K^+的通透性。利用大肠杆菌表达系统,通过Na^+激活K^+转运活性。
英文摘要
1. AtHKTl from Arabidopsis (AtHKTl) was found to form 4-fold membrane-pore-membrane structure.2. It is found that the P-loop of HKT transporters show identity only in one of glycine residues that mediates K^+ selectivity. HKT transporters share a common ancestor transporter with 4-loops.3. KATl shares typical similarity with other animal and plant K^+ channels. The voltage-sensing segments of S3 and S4 in KATl are posttranslationally integrated into the membrane only when specific interation occurs between them. The insertion process is a newly recognized second type of the membrane protein integration process.4. The aspartate in the transmembrane of Na^+/H^+ antiporter from a bacteria involves in H^+ permeation but not Na^+ permeation.5. The AtHKTl was expressed in vascular tissue in plant.6. AtHKTl plays a role in the recirculation of Na^+ in plant body.7. The K^+ permeation of HKT-type transporter in Synechocystis PCC6803 was measured. K^+ transport activity was activated by Na^+ using E. coli expression system.
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Kato,Y., Sakaguchi,M., Mori Y., Saito,K., Nakamura,T., Bakker,E.P., Sato,Y., Goshima,S. and Uozumi,N.: "Evidence in support of a four transmembrane-pore-transmembrane topology model for the Arabidopsis thaliana Na^+/K^+ translocating AtHKTl protein, a mem
加藤,Y.,坂口,M.,森 Y.,斋藤,K.,中村,T.,巴克,E.P.,佐藤,Y.,五岛,S。
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通讯作者:
魚住 信之: "化学と生物(植物のK^+チャネル・K^+トランスポーターはどこまでわかったか)"5 (2001)
鱼住伸行:“化学与生物学(我们对植物中的 K^+ 通道和 K^+ 转运蛋白了解多少)”5 (2001)
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Sato,Y., Sakaguchi,M., Goshima,S., Nakamura,T., and Uozumi,N.: "Integration of Shaker-type K^+ channel, KATl into the endoplasmic reticulum membrane : Synergistic insertion of voltage sensing segments, S3-S4 and independent insertion of pore-forming segme
Sato,Y.、Sakaguchi,M.、Goshima,S.、Nakamura,T. 和 Uozumi,N.:“将 Shaker 型 K^ 通道、KAT1 整合到内质网膜中:电压传感段的协同插入,
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Master, P., et al.: "Altered shoot/root Na^+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na^+ transporter AtHKT1"FEBS Letters. 531. 157-161 (2002)
Master, P. 等人:“通过 Na+ 转运蛋白 AtHKT1 的遗传破坏改变了拟南芥中的芽/根 Na+ 分布和分叉盐敏感性”FEBS Letters。
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Sato,Y., Hosoo,Y., Sakaguchi,M., and Uozumi,N.: "(2003) Requirement of negative residues, Asp 95 and Asp 105, in S2 on channel function and membrane integration of voltage-dependent K^+ channel, KATl"Biosci.Biotech.Biochem.. 67. 923-926 (2003)
Sato,Y.、Hosoo,Y.、Sakaguchi,M. 和 Uozumi,N.:“(2003) S2 中负残基 Asp 95 和 Asp 105 对电压依赖性 K^ 的通道功能和膜整合的要求
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共 24 条
Elucidation of the Na and K transport system conferring salinity tolerance in plant cells
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批准号:22380056
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.65万
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财政年份:2010
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负责人:UOZUMI Nobuyuki
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依托单位:
Analysis of function of ion channel using giant bacteria and identification of membrane transporters
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批准号:19380058
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.49万
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财政年份:2007
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负责人:UOZUMI Nobuyuki
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依托单位:
Molecular analysis of ion transporters in plant and cyanobacteria involved in response to high osmolality
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批准号:17380064
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.46万
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财政年份:2005
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负责人:UOZUMI Nobuyuki
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依托单位:
Study on plant Na and K transport system, which is involved in the formation of membrane potential and adaptation of osmolality
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批准号:17078005
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$53.44万
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财政年份:2005
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负责人:UOZUMI Nobuyuki
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依托单位:
Molecular analysis of plant Na and K transporter involved in response to environmental stress
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批准号:15380070
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.53万
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财政年份:2003
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负责人:UOZUMI Nobuyuki
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依托单位:
Study on function of the diverse K^+ transporters from plants
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批准号:11660082
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.43万
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财政年份:1999
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负责人:UOZUMI Nobuyuki
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依托单位:
海外基金