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Development of the technology for the production of novel wood materials using radical-generating activity of ligninolytic enzymes of basidiarycetes.

利用担子纲木质素分解酶的自由基生成活性开发新型木质材料生产技术。

基本信息

批准号：
15380122
负责人：
KUWAHARA Masaaki
金额：
$ 7.49万
依托单位：
Institue of Wood Technology, Akita Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Screening of basidiomyceteous fungi, which are expected to apply to enzymatic treatment of wood meal. Coliolus consors (Usubatake), Cyatus stercoreus (Hatakechadaigoke) and other fungal strains were selected. Pleurotus ostreatus (Hiratake) and C. stercoreus were also selected as the fungi which caused oxidative degradation of 4-chlororesolsinol. C. sonsors was found to produce high level of laccase, which was purified to give two main isozymes.In an acidic condition, this laccase preparation polymerized 2, 6-dimethoxyphenol (DMF) to give oligomeric compounds which were not solubilized in organic solvents. Commercial Iaccase and i so I ated laccase were found to increase molecular size of alkaline lignin and lignosulfonate by using GPC. In this reaction, DMP was thought to act as the mediator to enhance the polymerization reaction of lignin preparations.Wood meal of Japanese cedar was treated with PSDR (Pressurized single drive-disk refiner) to prepare wood fiber. The fiber was treated with commercial laccase and prepared sheet specimen by using a hand press successively. I t was found that treatment of the fiber with laccase in the presence of the mediator (ABTS and HOW increased Young coefficient and extension strength of the sheet. Raman spectrometry showed formation of the signals caused by laccase reaction. ESR analysis also showed the increase in the peak strength contributed by the radial species. Thus, possibility of application of fungal ligninolytic enzymes including laccase to devlop the novel process of manufacturing fiber and particle boards was shown in this research.

担子菌的筛选，有望应用于木粉的酶处理。选择Coliolus consors （Usubatake）、Cyatus stercoreus （Hatakechadaigoke）等真菌菌株。平菇（Pleurotus ostreatus, Hiratake）和C. stercoreus是引起4-氯resolsinol氧化降解的真菌。发现C. sonsors产生高水平的漆酶，纯化得到两个主要的同工酶。该漆酶制剂在酸性条件下聚合2,6 -二甲氧基苯酚（DMF），得到不溶于有机溶剂的低聚化合物。用GPC对碱性木质素和木质素磺酸盐进行了改性，发现商品化木质素酶和改性木质素酶能使碱性木质素和木质素磺酸盐的分子量增大。在该反应中，DMP被认为是促进木质素制剂聚合反应的介质。采用加压单盘精炼机对杉木粉进行处理，制备木纤维。用工业漆酶对纤维进行处理，并用手压法制备板样。结果表明，在ABTS和HOW介质存在的情况下，漆酶处理纤维可提高纤维的杨氏系数和拉伸强度。拉曼光谱分析显示漆酶反应引起的信号形成。ESR分析还表明，径向树种对峰值强度的贡献有所增加。因此，本研究显示了应用漆酶等真菌木质素降解酶开发纤维和刨花板新工艺的可能性。