Development of the technology for the production of novel wood materials using radical-generating activity of ligninolytic enzymes of basidiarycetes.

担子菌类真菌的筛选，有望应用于木粉的酶处理。选择了Coliolus Consor(Usubatake)、Cyatus stercoreus(Hatakechadaigoke)等真菌菌株。平菇(Pleurotus ostreatus(Hiratake))和斯特氏侧耳(C.stercoreus)也被选为氧化降解4-氯解醇的真菌。在酸性条件下，该漆酶制剂聚合2，6-二甲氧基苯酚(DMF)，得到不溶于有机溶剂的低聚物。利用凝胶渗透色谱(GPC)研究了商品漆酶和封闭漆酶对碱木素和木质素磺酸盐分子尺寸的影响。在该反应中，DMP被认为是促进木素制备聚合反应的中间体。用PSDR(加压单盘磨浆机)对日本雪松木粉进行处理，制备木纤维。用市售漆酶对纤维进行处理，并用手压法制备了片状样品。发现在介体(ABTS)存在的情况下用漆酶处理纤维，以及如何增加纸张的杨氏系数和拉伸强度。拉曼光谱显示由漆酶反应引起的信号的形成。ESR分析还表明，放射状物种对峰强度的贡献较大。因此，本研究为利用包括漆酶在内的真菌木质素分解酶开发制造纤维和刨花板的新工艺提供了可能性。

Screening of basidiomyceteous fungi, which are expected to apply to enzymatic treatment of wood meal. Coliolus consors (Usubatake), Cyatus stercoreus (Hatakechadaigoke) and other fungal strains were selected. Pleurotus ostreatus (Hiratake) and C. stercoreus were also selected as the fungi which caused oxidative degradation of 4-chlororesolsinol. C. sonsors was found to produce high level of laccase, which was purified to give two main isozymes.In an acidic condition, this laccase preparation polymerized 2, 6-dimethoxyphenol (DMF) to give oligomeric compounds which were not solubilized in organic solvents. Commercial Iaccase and i so I ated laccase were found to increase molecular size of alkaline lignin and lignosulfonate by using GPC. In this reaction, DMP was thought to act as the mediator to enhance the polymerization reaction of lignin preparations.Wood meal of Japanese cedar was treated with PSDR (Pressurized single drive-disk refiner) to prepare wood fiber. The fiber was treated with commercial laccase and prepared sheet specimen by using a hand press successively. I t was found that treatment of the fiber with laccase in the presence of the mediator (ABTS and HOW increased Young coefficient and extension strength of the sheet. Raman spectrometry showed formation of the signals caused by laccase reaction. ESR analysis also showed the increase in the peak strength contributed by the radial species. Thus, possibility of application of fungal ligninolytic enzymes including laccase to devlop the novel process of manufacturing fiber and particle boards was shown in this research.