STUDIES ON THE APPLICATION OF FOCUSED SHOCK WAVES TO THE CANCER TREATMENT

本文研究了水下冲击波的生物学效应，并从以下几个方面探讨了水下冲击波应用于癌症治疗的可能性：1)水下冲击波对正常犬肾脏的影响(原位)。2)聚焦冲击波对小鼠后垫和犬膀胱移植瘤的影响。SWS主要由24片压电陶瓷元件进行基因分选。通过超声诊断仪将目标对准焦点区域。1)在急性实验中，出血病变发生在与焦点区域完全对应的肾脏R1处。组织学观察显示血管壁(主要是动脉)主要受损。出血的病灶被瘢痕组织替代，持续1个月。经S超声观察，肾脏内有高回声区，全息干涉法显示，水中焦区有500毫秒的小气泡。这些结果提示空化损伤可能与组织损伤有关。2)微爆产生的高能冲击波可抑制小鼠乳腺癌(MM48)移植瘤到小鼠后垫的生长，但低能冲击波(300bar)不能抑制其生长。在V2肉瘤细胞移植的实验性膀胱肿瘤中，连续照射聚焦冲击波(1000bar，2000~6000次/天，2~5天)可引起明显的肿瘤坏死，并使肿瘤体积缩小。这些结果表明，一种利用聚焦冲击波治疗癌症的新方法可能被打开，如果超声可视化，实体瘤将在未来被体外治疗。

The biological effect of underwater shock waves (SWs) has been studied and the possibility of its application to the cancer treatment also investigated through the following categories: 1)Effects of SW on the normal dog kidney (in situ). 2)Effects of focused shock waves on the implanted carcinoma in the mouse hind pad and the dog urinary bladder. The SWs were mainly gene rated by a 24-piece piezoceramic element. Targeting an object to the focus region was performed by an ultrasonic siagnostic system.1)In acute experiment the bleeding lesion was developed in the kidney R 1 exactly corresponding to the focus area. Histological observations suggested that the vessel wall(mainly of arteries) was primarily injured. The bleeding lesion was replaced by the scarring tissue for 1 month. Under ultrasonic b s observation a hyperechoic region was observed in the kidney.Holographic interferometry disclosed that small bubbles remained for 500msec in the focal region in water. These results suggested that cavitation damage may relate to the tissue injury.2)Growth of implanted mouse mammary carinoma (MM48) to mouse hind pad was suppressed after an application of high energy shock wave generated by micro explosion but not inhibited by a low energy shock wave ((300 bar). In experimental bladder tumor of implantation of V2 carinoma cells the successive a exposure to the focused shock waves (1000 bar, 2000-6000 shots/day for 2-5 days) induced marked tumor necrosis and decreased in tumor size.It is concluded that the focused shock waves induce tumor necrosis and decrease of tumor size under the present experimental conditions. These re sults suggest that a new modality of cancer treatment using focused shock waves may be opened and a solid tumor, if ultrasonically visualized, be extracorporeally treated in future.