STUDIES ON THE APPLICATION OF FOCUSED SHOCK WAVES TO THE CANCER TREATMENT

聚焦冲击波在癌症治疗中的应用研究

• 批准号: 63480356
• 负责人: KUWAHARA Masaaki
• 金额: $ 3.84万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480356/
• 关键词: Shock wave; Biological effect; Cancer treatment; Implanted bladder tumor; Implanted food-pad tumor; Animal experiment; 集束衝撃波

The biological effect of underwater shock waves (SWs) has been studied and the possibility of its application to the cancer treatment also investigated through the following categories: 1)Effects of SW on the normal dog kidney (in situ). 2)Effects of focused shock waves on the implanted carcinoma in the mouse hind pad and the dog urinary bladder. The SWs were mainly gene rated by a 24-piece piezoceramic element. Targeting an object to the focus region was performed by an ultrasonic siagnostic system.1)In acute experiment the bleeding lesion was developed in the kidney R 1 exactly corresponding to the focus area. Histological observations suggested that the vessel wall(mainly of arteries) was primarily injured. The bleeding lesion was replaced by the scarring tissue for 1 month. Under ultrasonic b s observation a hyperechoic region was observed in the kidney.Holographic interferometry disclosed that small bubbles remained for 500msec in the focal region in water. These results suggested that cavitation damage may relate to the tissue injury.2)Growth of implanted mouse mammary carinoma (MM48) to mouse hind pad was suppressed after an application of high energy shock wave generated by micro explosion but not inhibited by a low energy shock wave ((300 bar). In experimental bladder tumor of implantation of V2 carinoma cells the successive a exposure to the focused shock waves (1000 bar, 2000-6000 shots/day for 2-5 days) induced marked tumor necrosis and decreased in tumor size.It is concluded that the focused shock waves induce tumor necrosis and decrease of tumor size under the present experimental conditions. These re sults suggest that a new modality of cancer treatment using focused shock waves may be opened and a solid tumor, if ultrasonically visualized, be extracorporeally treated in future.

人们已经研究了水下冲击波（SW）的生物学效应，并通过以下类别研究了其应用于癌症治疗的可能性：1）SW对正常狗肾脏（原位）的影响。 2)聚焦冲击波对小鼠后垫和狗膀胱植入癌的影响。 SW 主要由 24 片压电陶瓷元件产生。通过超声诊断系统将物体瞄准焦点区域。1)在急性实验中，在与焦点区域精确对应的肾脏R 1 中出现出血病变。组织学观察表明，血管壁（主要是动脉）主要受到损伤。 1个月后，出血灶被疤痕组织取代。超声观察肾脏内可见强回声区。全息干涉测量发现水中焦点区域有小气泡持续500msec。这些结果表明，空化损伤可能与组织损伤有关。2)在施加微爆炸产生的高能冲击波后，小鼠后垫上植入的小鼠乳腺癌(MM48)的生长受到抑制，但低能冲击波(300 bar)则不受抑制。在植入V2癌细胞的实验性膀胱肿瘤中，连续暴露于聚焦冲击波(1000 bar， 2000-6000次/天，持续2-5天）诱导显着的肿瘤坏死和肿瘤尺寸减小。得出的结论是，在本实验条件下，聚焦冲击波诱导肿瘤坏死和肿瘤尺寸减小。这些结果表明，使用聚焦冲击波治疗癌症的新方式可能会被开辟，并且如果超声可视化，实体瘤将来可以进行体外治疗。

项目成果

Hezabel,J.E.et al: "The effect of low energy underwater shockwaves on the implanted mouse mammary adenocarcinona cells" Tohohu J.Exp.Med.(1990)

Hezabel,J.E.等人：“低能量水下冲击波对植入的小鼠乳腺腺癌细胞的影响”Tohohu J.Exp.Med.(1990)

桑原正明ら: "体結石破砕におけるキャビテ-ションの研究:生体内気泡発生の可能性" 医用電子と生体工学. 27. 27 (1989)

Masaaki Kuwahara 等人：“结石破碎中的空化研究：体内气泡产生的可能性”《医疗电子和生物工程》27. 27 (1989)。

星宣次ら: "家兎VX2膀胱癌に対する高エネルギ-水中衝撃波の効果" 第一回バイエンジニアリングシンポジウム論文集. 1. 83-84 (1990)

Nobuji Hoshi 等人：“高能水下冲击波对家兔 VX2 膀胱癌的影响”第一届双工程研讨会论文集 1. 83-84 (1990)。

Kawahara,M.et al.: "Hyperechoic region induced by focused shock wowes:posssibility of acoustic caritation bubbles" J.Lithotripy stone Disease. 1. 282-288 (1989)

Kawahara，M.等人：“聚焦冲击波引起的高回声区域：声学气泡的可能性”J.碎石术结石疾病。

Hezabel,J.E.,et al.: "The effect of lowenergy underwater shock wowes on the implanted Mouse mammary adenocarcinoma cells" Tohoku J.Exp.Med(投稿中). (1990)

Hezabel, J.E. 等人：“低能量水下冲击对植入的小鼠乳腺癌细胞的影响”Tohoku J.Exp.Med（进行中）。