Production of Novel Polymers Using Lignin-degrading Peroxidases Produced by Wood-rot Basidiomycete

利用木质素分解担子菌产生的锰过氧化物酶，对愈创木酚进行聚合制备甲醇不溶性产物。对聚愈创木酚进行IR和NMR分析，发现除联苯外，聚愈创木酚还含有醚键，羟基部分含量较少。MULDI-TOF-MS分析表明，该聚合物的基本单元为愈创木酚，质量平均分子量在1200左右，最高分子量为3200。热分析表明，该聚合物没有明确的熔点和分级点。丙烯酰胺也聚合成聚合物，聚丙烯酰胺。核磁共振分析表明，该聚合物的构型为无规构型。漆酶也被发现由毛色Coriolus vericolus coccineus产生，可以从酚类化合物中产生凝胶。发现这个反应在丙酮水溶液中进行。采用苯酚作为液化介质，对桦木粉进行了液化。该产品与Novolak等符合JIS标准的酚醛树脂具有相似的性能。

By using manganese peroxidase produced by ligninolytic basidiomycete, guaiacol was polymerized to produce methanol insoluble products. IR and NMR analysis of the polyguaiacol, it was found that polyguaiacol contained ether linkages besides biphenyl linkage, with less content of hydroxyl moiety. MULDI-TOF-MS analysis showed that basic unit of the polymer was guaiacol and the weight average molecular weight was around 1200 with the highest Mw was 3200. Thermal analysis indicated that the polymer did not have clear melting and grassification points. Acrylamide also polymerized to give the polymer, polyacryl amide. NMR analysis showed that configuration of the polymer was atactic. Laccase produced by Coriolus vericolor and Pycnoporus coccineus was also found to produce gels from fenolic compounds. This reaction was found to proceed in aqueous acetone.Wood meal of birch was liquefied by using phenol medium. This product showed the similar characteristics with those of phenol resin such as Novolak which meets to JIS.