Production of Novel Polymers Using Lignin-degrading Peroxidases Produced by Wood-rot Basidiomycete

利用木腐担子菌产生的木质素降解过氧化物酶生产新型聚合物

• 批准号: 10460073
• 负责人: KUWAHARA Masaaki
• 金额: $ 8万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460073/
• 关键词: peroxidase; manganese peroxidase; laccase; lignin-degrading enzyme; phenolic polymer; Basidiomycetes; Bjerkandera adusta; Coriolus versicolor

By using manganese peroxidase produced by ligninolytic basidiomycete, guaiacol was polymerized to produce methanol insoluble products. IR and NMR analysis of the polyguaiacol, it was found that polyguaiacol contained ether linkages besides biphenyl linkage, with less content of hydroxyl moiety. MULDI-TOF-MS analysis showed that basic unit of the polymer was guaiacol and the weight average molecular weight was around 1200 with the highest Mw was 3200. Thermal analysis indicated that the polymer did not have clear melting and grassification points. Acrylamide also polymerized to give the polymer, polyacryl amide. NMR analysis showed that configuration of the polymer was atactic. Laccase produced by Coriolus vericolor and Pycnoporus coccineus was also found to produce gels from fenolic compounds. This reaction was found to proceed in aqueous acetone.Wood meal of birch was liquefied by using phenol medium. This product showed the similar characteristics with those of phenol resin such as Novolak which meets to JIS.

利用木质素降解菌产生的锰过氧化物酶聚合愈创木酚，得到甲醇不溶产物。通过对聚愈创木酚的红外光谱和核磁共振谱分析，发现聚愈创木酚中除联苯外还含有醚键，羟基含量较少。MULDI-TOF-MS分析表明，聚合物的基本单元为愈创木酚，重均分子量约为1200，最高Mw为3200。热分析表明，聚合物没有明确的熔点和塑化点。丙烯酰胺也聚合得到聚合物，聚丙烯酰胺。核磁共振分析表明聚合物的构型为无规立构。由Coriolus vericolor和Pycnoporus coccineus产生的漆酶也被发现从酚类化合物产生凝胶。该反应在丙酮水溶液中进行，白桦木粉在苯酚介质中液化。该产品与符合JIS标准的酚醛清漆等酚醛树脂具有相似的特性。

项目成果

S.Yoshida, Y.Chatani, Y.Honda, T.Watanabe, M.Kuwahara: "Reaction of manganese-dependent peroxidase from Bjerkandera adusta in aqueous organic media"J. Molecular Catalysis B : Enzaymatic. 9. 173-182 (2000)

S.Yoshida，Y.Chatani，Y.Honda，T.Watanabe，M.Kuwahara：“来自 Bjerkandera adusta 的锰依赖性过氧化物酶在水性有机介质中的反应”J。

K.Iwahara, Y.Honda, T.Watanabe, M.Kuwahara: "Polymerzation of guaiacol by lignin-degrading manganese peroxidase from Bjerkandera adusta in aqueous organic solvents"J. Microbiol. Biotech.. 52. (2000)

K.Iwahara、Y.Honda、T.Watanabe、M.Kuwahara：“来自 Bjerkandera adusta 的木质素降解锰过氧化物酶在水性有机溶剂中聚合愈创木酚”J。

T.Watanabe,K.Koller,K.Messner: "Copper-dependent depolymerization of lignin in the presence of fungal metabollte,pyrldine" J.Biotecnol. 62. 221-230 (1998)

T.Watanabe、K.Koller、K.Messner：“真菌代谢物吡啶存在下木质素的铜依赖性解聚”J.Biotecnol。

S.Yoshida, A.Chatani, Y.Honda, T.Watanabe, M.Kuwahara: "Reaction of manganese peroxidase of Bjerkandera adusta with synthetic lignin in acetone solution" J.Wood Sci. 44. 486-490 (1998)

S.Yoshida、A.Chatani、Y.Honda、T.Watanabe、M.Kuwahara：“Bjerkandera adusta 的锰过氧化物酶与丙酮溶液中的合成木质素的反应”J.Wood Sci。

K. Iwahara, Y. Honda, T. Watanabe, M. Kuwahara: "Polymerization of guaiacol by lignin-degrading manganese peroxidase from Bjerkandera adusta in aqueous organic solvents"J. Microbiol. Biotech.. 52. (2000)

K. Iwahara、Y. Honda、T. Watanabe、M. Kuwahara：“来自 Bjerkandera adusta 的木质素降解锰过氧化物酶在水性有机溶剂中聚合愈创木酚”J。