Production of ligninolytic enzymes and utilization of these enzymes to the conversion of wood biomass

木质素分解酶的生产以及利用这些酶转化木材生物质

• 批准号: 05556055
• 负责人: KUWAHARA Masaaki
• 金额: $ 8.45万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05556055/
• 关键词: Lignin-degrading; white-rot fungi; Lignin peroxidase; Mu(II)peroxidase; Bioreacter; Solid culture; 固体培養; N-アセチルグルコサミダーゼ; 水蒸気爆砕; 酵素糖化; リグノセルロース

This research aims to produce lignin-degrading enzymes by using various types of reacters which are possibly used for the conversion of lignocellulosic baiomass.Basidiomycetes were screened for the productivity of LiP and MnP on the wood meal medium. Bjerkandera adusta produced LiP and MnP on wood meal added with wheat bran as a nutrient in flasks efficiently. These enzymes were also produced by using rotary solid reacter. Production of ligninolytic enzymes was shown to be effected by the moisture content of the medium. Pleurotus ostreatus produced LiP and MnP in schaving-brau in this reacter.MnP produced in the solid culture was purified and characterized. An air-lift type reacter was found to be useful for the production of MnP by B.adusta. These results suggest that scale up of the production of ligninolytic enzymes is possible by using bench-scale reacters.LiP purified from the culture of Phanerochaete chrysosporium was modified by the derivarives of PEG.The native and modified LiP oxidized the substrate effectively in ethylene glycol and other organic solvents.Cellulosic components in wood meal treated with steam explosion was well hydrolyzed by cellulase of Trichodema Treatment with P.chrysosporium of wood meal previous to explosion enhanced the hydrolysis.

本研究旨在利用各种类型的反应器生产木质素降解酶，以转化木质纤维素生物质。在木粉中添加麦麸作为营养物，在摇瓶中，烟杆草能够有效地产生LiP和MnP。采用旋转式固体反应器进行了酶的生产。木质素降解酶的生产被证明是受介质的水分含量。在此反应器中，平菇在Schaving-brau培养基中发酵生产LiP和MnP，并对固体发酵生产的MnP进行了纯化和表征。气升式反应器被发现是有用的生产锰磷酸锰由B. adusta。这些结果表明，通过使用台架发酵，木质素分解酶的生产规模扩大是可能的。用PEG衍生物对从黄孢原毛平革菌培养物中纯化的LiP进行了改性，天然LiP和改性LiP在乙二醇等有机溶剂中对底物有较好的氧化作用，木粉经蒸汽爆破处理后，其纤维素组分在木粉中被木霉纤维素酶很好地水解在爆炸前加入木粉可增强水解作用。

项目成果

Y.Kimura: "Immunological test using Antiserum to lignin peroxidase of Bjerkandera adusta" Mokuzai Gakkaishi. 40(印刷中). (1994)

Y. Kimura：“使用 Bjerkandera adusta 木质素过氧化物酶抗血清进行免疫学测试”Mokuzai Gakkaishi 40（出版中）。

Tatsuro Sawada: "Effect offungal pretreatment and steam explosion pretreatment on enzymatic saccharification of plant biomass" Biotechnol.Bioeng. in press.

Tatsuro Sawada：“真菌预处理和蒸汽爆破预处理对植物生物质酶促糖化的影响”Biotechnol.Bioeng。

Yasuhiko Asada: "Purification and characterization of aryl-alcohol oxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium" Bios.Biotech.Biochem. in press.

Yasuhiko Asada：“来自木质素降解担子菌、Phanerochaete chrysosporium 的芳基醇氧化酶的纯化和表征”Bios.Biotech.Biochem。

M.Taniguchi: "Production of Enzymes for Food Processing by Solid State Fermentation" Proceeding of The Sixth International Congress on Engeneering and Food. 531-533 (1994)

M.Taniguchi：“通过固态发酵生产食品加工酶”第六届国际工程与食品大会论文集。

麻田恭彦: "Purification and characterization of aryl-alcohol oxidase from the lignin-degrading basidiomycete,Phanerochaete chrysosporium" Bios.Biotech.Biochem.59(印刷中). (1995)

Yasuhiko Asada：“来自木质素降解担子菌、Phanerochaete chrysosporium 的芳基醇氧化酶的纯化和表征”Bios.Biotech.Biochem.59（出版中）。