Studies on Production of Methane from Wood Biomass by Anaerobic Fermentation and Mechanism of Gas Production

利用城市污水处理厂厌氧生物反应器中的污泥，研究了木材生物质产气的情况。葡萄糖被认为是良好的底物生产的气体，其中包括甲烷在厌氧条件下。还从纤维素（Avicel）和滤纸获得气体。通过污泥对纤维素的驯化，提高了纤维素和纸张的产气率。研究了木质材料的机械和生物预处理对这些材料产气的影响。还通过处理过的木材材料的酶糖化来估计效果。细磨处理对日本雪松和山毛榉木材的糖化和产气都有很好的效果;山毛榉木材经20和60分钟的处理，还原糖的产率达到综纤维素的近90%，厌氧气体的转化率分别为综纤维素的近60%和80%。然而，与研磨机相比，精磨机处理的效果较差。结果表明，毛孢和黑蛋巢菌对山毛榉木材的糖化和产气均有较好的促进作用。两种革盖菌的糖化率和气体转化率最高。

Production of gas from wood biomass was examined using sludge obtained from anaerobic digestor in a municipal wastewater treatment plant. Glucose was found to be good substrate for the production of gas which included methane in anaerobic condition. Gas was also obtained from cellulose (Avicel) and filter paper. Production of gas from cellulose and paper was enhanced by acclimatization of the sludge to cellulose.Effects mechanical and biological pretreatments of wood materials on gas production from these materials were examine 1. The effects were also estimated by enzymatic saccharification of the treated wood materials. Treatment by a fine grinder gave exelent effects both on saccharification and gas production from Japanese cedar and beech wood; yield of reducing sugar reached to almost 90% of holocellulose and conversion rate to anaerobic gas was almost 60 and 80% of holocellulose by the treatment of 20 and 60 minutes respectively in the case of beech wood. However, treatment by a refiner was less effective compared to that by a grinder.Fungal treatments using Coriolus consors, C. hirsutus and Cyathus stercores gave good effect on both saccharification and gas production from beech wood. Maximal saccharification yield and gas conversion rate were obtained by the treatment using two Coriolus species tested.