Studies on Production of Methane from Wood Biomass by Anaerobic Fermentation and Mechanism of Gas Production

利用城市污水处理厂厌氧沼气池产生的污泥，对木材生物质产气进行了研究。葡萄糖是厌氧条件下生产甲烷等气体的良好底物。还可以从纤维素（Avicel）和滤纸中获得气体。污泥对纤维素的驯化提高了纤维素和纸张的产气率。考察了木质材料的机械和生物预处理对这些材料产气的影响。对处理后的木质材料进行了酶糖化处理，并对效果进行了评价。细磨机处理对杉木和山毛榉木的糖化和产气都有很好的效果；以山毛榉木为研究对象，经过20分钟和60分钟的处理，还原糖的产率可达近90%的纤维素，厌氧气的转化率可达近60%和80%的纤维素。然而，与研磨机相比，精炼机的处理效果较差。真菌对山毛榉木糖化和产气均有较好的影响。采用两种科氏菌处理，获得了最大的糖化率和气体转化率。

Production of gas from wood biomass was examined using sludge obtained from anaerobic digestor in a municipal wastewater treatment plant. Glucose was found to be good substrate for the production of gas which included methane in anaerobic condition. Gas was also obtained from cellulose (Avicel) and filter paper. Production of gas from cellulose and paper was enhanced by acclimatization of the sludge to cellulose.Effects mechanical and biological pretreatments of wood materials on gas production from these materials were examine 1. The effects were also estimated by enzymatic saccharification of the treated wood materials. Treatment by a fine grinder gave exelent effects both on saccharification and gas production from Japanese cedar and beech wood; yield of reducing sugar reached to almost 90% of holocellulose and conversion rate to anaerobic gas was almost 60 and 80% of holocellulose by the treatment of 20 and 60 minutes respectively in the case of beech wood. However, treatment by a refiner was less effective compared to that by a grinder.Fungal treatments using Coriolus consors, C. hirsutus and Cyathus stercores gave good effect on both saccharification and gas production from beech wood. Maximal saccharification yield and gas conversion rate were obtained by the treatment using two Coriolus species tested.