Development of combination vaccine by using cytokine expressing transgenic plants.

利用表达细胞因子的转基因植物开发联合疫苗。

• 批准号: 15380198
• 负责人: MATSUMURA Takeshi
• 金额: $ 8.9万
• 依托单位: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380198/
• 关键词: cytokine; rotavirus; transgenic plants

Rotavirus is one of the most significant pathogens causing diarrhea in mammals. We have already produced the transgenic potato plants expressing rotavirus VP6 cDNA gene which encodes a coat protein. In addition, we have confirmed the antigenicity of the transgenic potato in mice.In this study we produced the transgenic strawberries which were introduced human interferon(hIFN) gene. Also we have produced the transgenic strawberry expressing both bovine IL-1 and rotavirus VP6 protein by using co-transformation technique. And here we examined the effect of oral administration to mice with combinations of vaccine and cytokine, using the transgenic strawberries and/or the transgenic VP6 potato mentioned above.BALB/c (8 week-old) mice were spontaneously fed with the transgenic plants once in a week, three times. After 1 week of the last(3rd) administration, those mice were challenged using rotavirus EW strain (murine group A) with dose of 10^5 TCID50.Virus antigen and anti-virus antibody in blood and feces of the challenged mice were examined. Secretory IgA was detected in feces of VP6 administrated mice. On the other hand, there was no significant difference in immune-markers among the hlFN strawberry, the IL-1 strawberry and non lransgenic strawberry administrated groups ;

轮状病毒是引起哺乳动物腹泻的重要病原体之一。我们已经获得了表达轮状病毒VP 6基因的转基因马铃薯植株。另外，我们还在小鼠体内证实了转基因马铃薯的抗原性。利用共转化技术获得了同时表达牛IL-1和轮状病毒VP 6蛋白的转基因草莓。在此，我们使用上述转基因草莓和/或转基因VP 6马铃薯，检测了将疫苗和细胞因子组合口服给药于小鼠的效果。末次（第3次）给药1周后，用轮状病毒EW株（小鼠A组）以10^5 TCID 50的剂量攻毒，检测攻毒小鼠血液和粪便中的病毒抗原和抗病毒抗体。在VP 6给药小鼠的粪便中检测到分泌型伊加。另一方面，hlFN草莓、IL-1草莓和非转基因草莓施用组之间的免疫标记物没有显著差异;

项目成果

Increase of antibody titer against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

• DOI: 10.1292/jvms.67.211
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ito, A;Gotanda, T;Matsumura, T
• 通讯作者: Matsumura, T

Increase of Antibody Titer against Leucocytozoon caulleryj by Oral Administration of Recombinant R7 Antigen.

口服重组 R7 抗原可增加抗白细胞虫的抗体滴度。

• 发表时间: 2005
• 期刊: J.Vet.Med.Sci 67(2)
• 作者: Akira Ito;Tohru Gotand;Shigeki Kobayashi;Katsumi Kume;Chihiro Sugimoto;Takeshi Matsumura
• 通讯作者: Takeshi Matsumura

Mammlian Cytokines Produced in pints.

哺乳动物细胞因子 以品脱量生产。

• 发表时间: 2004
• 期刊: Encyclopedia of Plant and Crop Science (MRCEL DEKKER, Inc) (In press)
• 作者: Chihiro Sugimoto;Takeshi Matsumura
• 通讯作者: Takeshi Matsumura

Ability of Orally Administered IFN-α containing Transgenic Potato Extracts to Inhibit Ldsteria monocytogenes Infection.

口服含有 IFN-α 的转基因马铃薯提取物抑制单核细胞增生李斯特氏菌感染的能力。

• 期刊: Journal of Interferon & Cytokine Research (In press)
• 作者: Kenji Ohya;Takeshi Matsumura.Noriko Itchoda;Kazuhiko Ohashi;Misao Onuma;Chiro Sugimoto
• 通讯作者: Chiro Sugimoto