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Possible roles of Runx1 in the incipient intramembranous ossification

Runx1 在早期膜内骨化中的可能作用

基本信息

批准号：
15390635
负责人：
YAMASHIRO Takashi
金额：
$ 9.54万
依托单位：
OSAKA UNIVERSITY (2004)Okayama University (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390635/
关键词：
Runx1 Cbfa1 Runx2 Osf2 intramembranous ossification Craniofacial cartilage bone Sox9 RUNX 1 Cbfa 1 ノックアウトマウス

项目摘要

Runx1, a gene essential for hematopoiesis, contains RUNX binding sites in its promoter region, suggesting possible cross-regulation with Runx2 and potential regulatory roles in bone development. On the other hand, Sox9 is essential for chondrogenesis, and haploinsufficiency of Sox9 leads to premature ossification of the skeletal system. In this study, we studied the possible roles of Runx1 and Sox9 in bone development. Runx1,Runx2/Osf2, and Sox9 expression was evaluated by in situ hybridization in the growing craniofacial bones of embryonic day (E)12-16 mice and in the endochondral bone-forming regions of embryonic and postnatal long bones. In addition, we evaluated Runx2/Osf2 expression in the growing face of Runx1 knockout mice at E12.5 and Runx1 expression in Runx2 knockout mice at E14.5. Runx1 and Sox9 were expressed in cartilage, and the regions of expression expanded to the neighboring Runx2-expressing osteogenic regions. Expression of both Runx1 and Sox9 was markedly downregulated on ossification. Runx1 and Sox9 expression was absent in the regions of endochondral bone formation and in actively modeling or remodeling bone tissues in the long bones as well as in ossified craniofacial bones. Runx2 expression was not affected by gene disruption of Runx1, whereas the expression domains of Runx1 were extended in Runx2(-/-) mice compared with wildtype mice. Runx1 and Sox9 are specifically expressed in the osteogenic cell compartments in the craniofacial bones and the bone collar of long bones, and this expression is downregulated on terminal differentiation of osteoblasts. Our results suggest that Runx1 may play a role in incipient intramembranous bone formation.

Runx 1是造血必需基因，在其启动子区含有RUNX结合位点，表明可能与Runx 2交叉调节，并在骨发育中具有潜在的调节作用。另一方面，Sox 9是软骨形成所必需的，Sox 9的单倍不足导致骨骼系统过早骨化。在这项研究中，我们研究了Runx 1和Sox 9在骨发育中的可能作用。Runx 1，Runx 2/Osf 2，和Sox 9的表达进行了评估，在胚胎（E）12-16天的小鼠生长颅面骨和在软骨内骨形成区域的胚胎和出生后的长骨原位杂交。此外，我们评估了Runx 2/Osf 2在E12.5时Runx 1敲除小鼠生长面中的表达，以及Runx 2敲除小鼠在E14.5时Runx 1的表达。Runx 1和Sox 9在软骨中表达，并且表达区域扩展到邻近的Runx 2表达成骨区域。Runx 1和Sox 9的表达在骨化过程中均显著下调。Runx 1和Sox 9的表达是不存在的区域内软骨骨形成和积极建模或重塑骨组织中的长骨以及在骨化颅面骨。Runx 2的表达不受Runx 1基因破坏的影响，而Runx 1的表达域在Runx 2（-/-）小鼠与野生型小鼠相比有所扩展。Runx 1和Sox 9在颅面骨和长骨骨领的成骨细胞室中特异性表达，并且这种表达在成骨细胞的终末分化中下调。我们的研究结果表明，Runx 1可能在早期膜内骨形成中发挥作用。

项目成果

期刊论文数量（22）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Possible Roles of Runx1 and Sox9 in Incipient Intramembranous Ossification

DOI：
10.1359/jbmr.040801
发表时间：
2004-10
期刊：
Journal of Bone and Mineral Research
影响因子：
6.2
作者：
T. Yamashiro;Xiu-ping Wang;Zhe Li;Shinji Oya;T. Åberg;T. Fukunaga;H. Kamioka;N. Speck;T. Takano-Yamamoto;I. Thesleff
通讯作者：
T. Yamashiro;Xiu-ping Wang;Zhe Li;Shinji Oya;T. Åberg;T. Fukunaga;H. Kamioka;N. Speck;T. Takano-Yamamoto;I. Thesleff

Terminal differentiation of osteoblasts to osteocytes is accompanied by dramatic changes in the distribution of actin-binding proteins

DOI：
10.1359/jbmr.040128
发表时间：
2004-03-01
期刊：
JOURNAL OF BONE AND MINERAL RESEARCH
影响因子：
6.2
作者：
Kamioka, H;Sugawara, Y;Takano-Yamamoto, T
通讯作者：
Takano-Yamamoto, T

Possible roles of Runx1 and Sox9 in the incipient intramembranous ossification

Runx1 和 Sox9 在早期膜内骨化中的可能作用

DOI：
发表时间：
2004
期刊：
Journal of Bone and Mineral Research 19(10)
影响因子：
0
作者：
Yamashiro T;Wang X;Zhen L;Oya S;Aberg T;Fukunaga T;Kamioka H;Speck NA;Takano-Yamamoto T;Thesleff I
通讯作者：
Thesleff I

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