Metabolic state dependence of olfactory cortex flavor representation during hedonic overeating

享乐性暴饮暴食期间嗅觉皮层风味表征的代谢状态依赖性

• 批准号: 458236353
• 负责人: Privatdozent Dr. Friedrich Johenning
• 金额: --
• 依托单位: Neurowissenschaftliches Forschungszentrum (CCM)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/458236353?language=en
• 关键词: Metabolic; state; dependence; olfactory; cortex

Obesity is a central public health issue in western society. An underlying problem is the uncoupling of caloric intake and metabolic needs of the organism during hedonic overeating. Hedonic overeating can escalate into the symptom of binge eating. One factor that modulates the feedback loop of metabolic need and caloric intake is the sensory perception of flavors. Olfaction is a critical part of flavor perception and significantly influences the hedonic evaluation food items. However, it is unclear how adaptations related to the transition from hedonic overeating to binge eating are reflected by the olfactory flavor response. This proposal therefore aims to understand the relationship between olfactory sensory perception and binge eating. The piriform cortex (PCx) is the first cortical olfactory processing stage where synthetic odor images are generated and odors are identified. Here, we will analyse the multimodal flavor response in the anterior PCx. We want to understand the relationship between the sensory representation of flavors during binge eating in different specific anterior PCx (aPCx) circuits and satiation at different metabolic states. The main underlying question is whether the amount of food consumption is modulated by sensory perception. We further want to test the hypothesis that the escalcation of binge eating and an obesity inducing diet will alter the sensory representation of flavors in the aPCx. The proposal requires the combination of in-vivo calcium imaging of neuronal activity in awake freely moving mice and a behavioral task. The behavioral task is designed to quantify consumption of high hedonic value food and escalate binge eating in real time during imaging sessions. For in vivo imaging of the lateroventral aPCx in awake freely-moving mice, microendoscopic lenses will be combined with a head-mounted, miniaturized microscope. Transgenic approaches will permit for cell-type specific expression of genetically encoded calcium indicators (GECIs). In a first step, we will analyze the circuit-specific changes of flavor representation in the aPCx during binge eating induction under different metabolic states. We will then test the effects of experimental manipulations of olfactory perception on dietary intake and flavor representation. Finally, we want to assess the changes in the dynamics of aPCX flavor representation during different time points of an obesity inducing diet. With this research proposal, we will make a significant contribution towards an understanding of the sensory underpinnings of dysregulated eating behavior.

肥胖是西方社会的一个中心公共卫生问题。一个潜在的问题是在享乐性暴饮暴食期间生物体的热量摄入和代谢需求的解耦。享乐性暴饮暴食会升级为暴饮暴食的症状。调节代谢需要和热量摄入的反馈回路的一个因素是对风味的感官感知。嗅觉是味觉感知的重要组成部分，对食物的感官评价有重要影响。然而，目前还不清楚如何适应有关的过渡，从享乐暴饮暴食的嗅觉反应反映。因此，这项建议旨在了解嗅觉感官知觉和暴饮暴食之间的关系。梨状皮质（PCx）是第一个皮质嗅觉处理阶段，合成气味图像的产生和气味被识别。在这里，我们将分析在前PCx的多模态风味反应。我们想了解在暴饮暴食期间，在不同特定的前PCx（aPCx）回路中的风味感觉表征与不同代谢状态下的饱足感之间的关系。主要的潜在问题是，食物消费量是否受感官知觉的调节。我们还想检验暴饮暴食和肥胖诱导饮食的升级将改变aPCx中风味的感官表征的假设。该提案需要结合清醒的自由移动小鼠和行为任务中的神经元活动的体内钙成像。行为任务被设计为在成像会话期间在真实的时间内量化高享乐值食物的消耗并升级暴食。对于清醒的自由移动小鼠的侧腹aPCx的体内成像，显微内窥镜镜头将与头戴式微型显微镜相结合。转基因方法将允许遗传编码的钙指示剂（GECIs）的细胞类型特异性表达。在第一步中，我们将分析在不同的代谢状态下，在暴食诱导期间，aPCx中的风味表征的回路特异性变化。然后，我们将测试嗅觉感知的实验操作对饮食摄入量和风味表征的影响。最后，我们想要评估在肥胖诱导饮食的不同时间点期间aPCX风味呈现的动态变化。通过这项研究计划，我们将为理解饮食行为失调的感官基础做出重大贡献。