Development of a novel organ preservation solution for the use of liver transplantation

探讨了缓冲液中加入重水进行简易冷藏的必要条件。新的解决方案似乎在各种细胞，大鼠肝脏和心脏中显示出优异的细胞保护作用，这是由于抑制细胞溶质Ca2+过载和细胞骨架破坏，以及刺激有氧呼吸。然而，这些有益的影响被取消在肝脏和肾脏保存和移植的狗，可能是由于刺激血管内凝集的PEG。为了进行临床应用，应探索PEG浓度的定制。此外，由于缓冲液中的重水主要作用是刺激好氧呼吸，我们应该找到更好的方法来提供氧气，而不凝集下一步。

We explored the necessary condition of heavy water containing buffer for the use of simple cold preservation. The novel solution appeared to show excellent cytoprotection in various cells, and rat liver and heart, due to the inhibition of cytosolic Ca2+ overload and cytoskeletal breakdown, and stimulation of aerobic respiration. However, these beneficial effects were abolished in liver and kidney preservation and transplantation of dogs, possibly due to the stimulation of intravascular agglutination by PEG. To proceed to the clinical application, customization of PEG concentration should be explored. Further, since the main action of heavy water containing buffer is stimulation of aerobic respiration, we should find better way to provide oxygen without agglutination for the next step.