Development of a novel organ preservation solution for the use of liver transplantation

探讨了简易冷藏使用含重水缓冲液的必要条件。由于抑制细胞内钙超载和细胞骨架的破坏，以及刺激有氧呼吸，该新型溶液在各种细胞以及大鼠的肝脏和心脏中表现出良好的细胞保护作用。然而，在狗的肝和肾的保存和移植中，这些有益的作用被消除了，可能是由于聚乙二醇刺激了血管内凝集。为了进行临床应用，应探索定制聚乙二醇浓度。此外，由于含有重水的缓冲液的主要作用是刺激有氧呼吸，因此我们应该为下一步寻找更好的供氧方式而不发生凝结。

We explored the necessary condition of heavy water containing buffer for the use of simple cold preservation. The novel solution appeared to show excellent cytoprotection in various cells, and rat liver and heart, due to the inhibition of cytosolic Ca2+ overload and cytoskeletal breakdown, and stimulation of aerobic respiration. However, these beneficial effects were abolished in liver and kidney preservation and transplantation of dogs, possibly due to the stimulation of intravascular agglutination by PEG. To proceed to the clinical application, customization of PEG concentration should be explored. Further, since the main action of heavy water containing buffer is stimulation of aerobic respiration, we should find better way to provide oxygen without agglutination for the next step.