Regulatory mechanisms in oral tissue-specific immune responses and tolerance

口腔组织特异性免疫反应和耐受性的调节机制

• 批准号: 23249082
• 负责人: AZUMA Miyuki
• 金额: $ 30.53万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23249082/
• 关键词: 免疫学; 歯学; 樹状細胞; 口腔粘膜; 歯髄; 歯肉; 免疫制御; 舌下免疫療法; 歯周病; 歯髄炎

We investigated oral immune responses induced by oral mucosal or dental pulpal DCs and epithelial cells, and found following new observations; 1) The density of oral mucosal DCs was clearly lower. 2) The density of sublingual DCs was markedly lower than those of buccal mucosa and dorsal surface of the tongue. 3) A comparable recruitment of submucosal DCs (smDCs) in the sublingual mucosa (SLM) was induced at an early phase after an initial hapten painting. 4) Repeated antigen painting onto SLM induced exhaustion of smDCs and conversion of macrophage-like smDC that possess less dendrites and round morphology with high levels of CD11b and CD206. 5) Dental pulp DCs migrated into regional lymph nodes after cusp trimming and possess mature phenotype. 6) Prickle cells in gingiva and dorsal surface of tongue constitutively expressed an immune regulatory molecule B7-H1. 7) B7-H1 that was overexpressed in gingival keratinocytes inhibited P.g-induced gingival inflammation and alveolar bone loss.

我们研究了口腔粘膜或牙髓DC和上皮细胞诱导的口腔免疫应答，发现以下新的观察结果：1）口腔粘膜DC的密度明显降低。2)舌下DCs密度明显低于颊粘膜和舌背表面DCs密度。3)舌下粘膜（SLM）中粘膜下DC（smDC）的可比较募集在初始半抗原涂敷后的早期阶段诱导。4)在SLM上重复抗原涂敷诱导smDC耗尽和巨噬细胞样smDC的转化，所述smDC具有较少的树突和圆形形态，具有高水平的CD 11b和CD 206。5)牙髓DC在牙尖修剪后可迁移至区域淋巴结，具有成熟的表型。6)牙龈和舌背表面的棘细胞组成性表达免疫调节分子B7-H1。7)牙龈角质形成细胞中过表达的B7-H1抑制了P. g诱导的牙龈炎症和牙槽骨丢失。

项目成果

T cell-dependent and -independent regulatory roles of a new inhibitory molecule VISTA

新型抑制分子 VISTA 的 T 细胞依赖性和非依赖性调节作用

• 发表时间: 2014
• 作者: Ohno T;Kondo Y;Zhang C;Azuma M
• 通讯作者: Azuma M

Involvement of PD-1 and B7-H1 in acute and chronic inflammation in periodontal disease

PD-1和B7-H1参与牙周病急慢性炎症

• 发表时间: 2012
• 作者: Maekawa S;Ohno T;Kobayashi H;Nakagawa I;Izumi Y;Azuma M
• 通讯作者: Azuma M

Establishment of a murine model for Japanese cedar pollinosis that manifests various symptoms like rhinitis, dermatitis, and asthma

建立表现鼻炎、皮炎、哮喘等多种症状的日本柳杉花粉症小鼠模型

• 发表时间: 2012
• 作者: Yanagisawa S;Zhang C;Tomoda T;Azuma M
• 通讯作者: Azuma M

Dynamics oforal mucosal dendritic cells after antigen application.

应用抗原后口腔粘膜树突状细胞的动态。

• 发表时间: 2012
• 作者: Zhang C;Ohno T;Azuma M.
• 通讯作者: Azuma M.

Dual functions of PD-1 in CD4+ T cell responses by sublingual mucosa-mediated antigen application

PD-1 在舌下粘膜介导的抗原应用 CD4 T 细胞应答中的双重功能

• 发表时间: 2012
• 作者: Ohashi Y;Iijima H;Yamaotsu N;Yamazaki K;Sato S;Okamura M;Sugimoto K;Dan S;Hirono S;Yamori T;ジュリア・ヨング;Matsuoka S;Yamori T;Ohno T
• 通讯作者: Ohno T