Realization of a Super High-rate Methane Recovery Technology from Waste and Wastewater.

实现废物废水中超高速甲烷回收技术。

基本信息

批准号：
11558073
负责人：
HARADA Hideki
金额：
$ 8.58万
依托单位：
Nagaoka University of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558073/
关键词：
UASB process Thermophilic methane fermentation Anaerobic wastewater treatment Granular sludge Methaiiogen Pilotscale FISH 16S rRNA

项目摘要

Whole cell in-situ hybridization with 16S rRNA targeted oligonucleotides was used to clarify the microbial ecological structure of sludge granules developed in laboratory- and pilot-scale upflow anaerobic sludge blanket (UASB) reactors receiving various types of wastewaters. Three different molecular probes : ARC915, complementary to conserve region of the 16S rRNA of the domain Archaea ; MT757, for genus Methanosaeta (Methanothrix), and MB1174, for Methanobacteriales, were employed in this study. The FISH results indicated that genera Archaea, Methanosaeta and Methanobacteriales accounted for approximately 30 to 60 %, 30 to 55%, and 10 to 30%, respectively of the DAPI (6 diamidino-2-phenylindole) stained cells (with responding to the total cell number) in the respective granular sludges.A strong correlation was observed between cell densities and methanogenic activities for both the Methanosaeta (acetate fed) and Methanobacteriales (hydrogen fed) genera. Topology of microbial structure of both the mesophilically- and thermophilically grown granules was investigated by dividing the individual whole granules into the outer-layer and the inner-layer portions. The methanogens were found more significant in the inner-layer portion. Similarly, thermophilic granules contained higher percentage of Methanobacter group that utilized hydrogen or formate for methanogenesis, as compared to the mesophilic. This suggests that syntrophic acetate oxidizing methanogenesis through hydrogen formation becomes more prominent under thermophilic conditions.

全细胞与16S rRNA靶向寡核苷酸的原位杂交用于阐明在实验室和试验尺度上流量表厌氧污泥毯（UASB）反应器中开发的污泥颗粒的微生物生态结构。三种不同的分子探针：ARC915，互补，以保护域古细菌的16S rRNA区域；本研究采用了MT757，用于甲藻类属（甲那诺氏菌（甲那刺）和MB1174，用于甲基细菌。鱼类的结果表明，古细菌，甲烷菌和甲基细菌属分别占DAPI（6个Diamidino-2-苯基吲哚）的大约30％至60％，30至55％和10％至30％的染色细胞（在各自的细胞数量中响应的GRANULUR SLUDINITIS）。甲烷酸（乙酸盐喂养）和甲基细菌（氢喂养）属。通过将整个整个颗粒分为外层和内层部分，研究了中粒和嗜热颗粒的微生物结构的拓扑结构。在内层部分发现甲烷剂更为重要。同样，与中嗜性相比，嗜热颗粒包含利用氢或甲酸甲烷的甲烷杆菌组较高的比例。这表明在嗜热条件下，通过氢形成氧化甲烷发生的甲烷生成变得更加突出。