Microbial Community Analyses of Municipal Solids Waste Landfill Site Soils by 16S rRNA molecular approach

建立了城市生活垃圾填埋场中10、20和30m深度的古细菌16S rDNA克隆文库，分别对应于大约3、6和9年的运行时间。对代表性克隆的测序和系统发育分析表明，所有rDNA都与典型产甲烷菌密切相关。各克隆文库的系统型分布基本一致。所有克隆文库中的优势克隆都与嗜热菌如甲烷热自养杆菌密切相关，表明这些地点的温度较高。依赖于H2的产甲烷活性测试结果支持了这一点，表明所有样品在55℃时的活性都远高于25℃时的活性。

Archaeal 16S rDNA clones libraries were constructed for samples taken at 10, 20 and 30m depth in a municipal solid waste landfill, which corresponded approximately 3, 6 and 9 years operation, respectively. Sequencing and phylogenic analyses of representative clones showed all of the rDNAs were closely related to typical methanogens. The distributions of phylotypes in clones libraries were similar to each other. Dominant clones in all the clone libraries were closely related to thermophilic species, such as Methanothermobacter thermoautotrophicus, suggesting that the temperature at these sites were high. This was supported by the results of H2-dependent methanogenic activity tests, showing that the activities of all samples at 55℃ were much higher than those at 25℃.