Microbial Community Analyses of Municipal Solids Waste Landfill Site Soils by 16S rRNA molecular approach

采用 16S rRNA 分子方法对城市固体废物填埋场土壤进行微生物群落分析

• 批准号: 13450214
• 负责人: HARADA Hideki
• 金额: $ 8.26万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13450214/
• 关键词: 16S rDNA; Archaea; diversity; landfill; Homoacetogenesis; *; Global warming; fluorescence in-situ hybridization; 温室化効果ガス

Archaeal 16S rDNA clones libraries were constructed for samples taken at 10, 20 and 30m depth in a municipal solid waste landfill, which corresponded approximately 3, 6 and 9 years operation, respectively. Sequencing and phylogenic analyses of representative clones showed all of the rDNAs were closely related to typical methanogens. The distributions of phylotypes in clones libraries were similar to each other. Dominant clones in all the clone libraries were closely related to thermophilic species, such as Methanothermobacter thermoautotrophicus, suggesting that the temperature at these sites were high. This was supported by the results of H2-dependent methanogenic activity tests, showing that the activities of all samples at 55℃ were much higher than those at 25℃.

分别从填埋场10、20和30 m深度取样，构建了填埋场运行3、6和9年的16 S rDNA克隆文库。对代表性克隆的测序和同源性分析表明，所有的rDNAs都与典型的产甲烷菌密切相关。各克隆文库中的多态性分布相似。所有克隆文库中的优势克隆都与嗜热物种密切相关，如Methanothermobacter thermoautotrophicus，这表明这些位点的温度很高。H2-依赖型产甲烷活性测试结果表明，所有样品在55℃下的产甲烷活性均远高于25℃。

项目成果

Imachi, H., Sekiguchi, Y., Kamagata, Y., Hanada, S., Ohashi, A., Harada H.: "Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium"Int. J. Syst. Evol. Microbiol.. Vol.52. 1729-1735 (2002

Imachi, H.、Sekiguchi, Y.、Kamagata, Y.、Hanada, S.、Ohashi, A.、Harada H.：“Pelotomaculum thermopropionicum gen. nov.，sp. nov.，一种厌氧、嗜热、互养的丙酸盐-

澤田浩介, 原田秀樹, 大橋晶良: "廃棄物埋立地模擬カラムによる有機物分解と温室効果ガス放出の挙動"第37回日本水環境学会年会講演集. Vol.37. 524 (2003)

Kosuke Sawada、Hideki Harada、Akiyoshi Ohashi：“使用废物填埋场模拟柱的有机物分解和温室气体释放行为”日本水环境学会第 37 届年会记录（2003 年）。

Y.Sekiguchi, Y.Kamagata, H.Harada: "Recent Advances in Methane Fermentation Technology"Current Opinion in Biotechnology. Vol.12 No.3. 277-282 (2001)

Y.Sekiguchi、Y.Kamagata、H.Harada：“甲烷发酵技术的最新进展”生物技术的当前观点。

Chen, A.-C., Ohashi, A, Harada.H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)

Chen, A.-C.、Ohashi, A、Harada.H.：“模拟和实际垃圾填埋场样品中 H2/CO2 合成乙酸盐”环境技术。

Chen, A.-C., Ohashi, A., Harada, H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)

Chen, A.-C.、Ohashi, A.、Harada, H.：“模拟和实际垃圾填埋场样品中 H2/CO2 合成乙酸盐”环境技术。