Microbial Community Analyses of Municipal Solids Waste Landfill Site Soils by 16S rRNA molecular approach
采用 16S rRNA 分子方法对城市固体废物填埋场土壤进行微生物群落分析
基本信息
- 批准号:13450214
- 负责人:
- 金额:$ 8.26万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Archaeal 16S rDNA clones libraries were constructed for samples taken at 10, 20 and 30m depth in a municipal solid waste landfill, which corresponded approximately 3, 6 and 9 years operation, respectively. Sequencing and phylogenic analyses of representative clones showed all of the rDNAs were closely related to typical methanogens. The distributions of phylotypes in clones libraries were similar to each other. Dominant clones in all the clone libraries were closely related to thermophilic species, such as Methanothermobacter thermoautotrophicus, suggesting that the temperature at these sites were high. This was supported by the results of H2-dependent methanogenic activity tests, showing that the activities of all samples at 55℃ were much higher than those at 25℃.
分别从填埋场10、20和30 m深度取样,构建了填埋场运行3、6和9年的16 S rDNA克隆文库。对代表性克隆的测序和同源性分析表明,所有的rDNAs都与典型的产甲烷菌密切相关。各克隆文库中的多态性分布相似。所有克隆文库中的优势克隆都与嗜热物种密切相关,如Methanothermobacter thermoautotrophicus,这表明这些位点的温度很高。H2-依赖型产甲烷活性测试结果表明,所有样品在55℃下的产甲烷活性均远高于25℃。
项目成果
期刊论文数量(28)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Imachi, H., Sekiguchi, Y., Kamagata, Y., Hanada, S., Ohashi, A., Harada H.: "Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium"Int. J. Syst. Evol. Microbiol.. Vol.52. 1729-1735 (2002
Imachi, H.、Sekiguchi, Y.、Kamagata, Y.、Hanada, S.、Ohashi, A.、Harada H.:“Pelotomaculum thermopropionicum gen. nov.,sp. nov.,一种厌氧、嗜热、互养的丙酸盐-
- DOI:
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- 影响因子:0
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澤田浩介, 原田秀樹, 大橋晶良: "廃棄物埋立地模擬カラムによる有機物分解と温室効果ガス放出の挙動"第37回日本水環境学会年会講演集. Vol.37. 524 (2003)
Kosuke Sawada、Hideki Harada、Akiyoshi Ohashi:“使用废物填埋场模拟柱的有机物分解和温室气体释放行为”日本水环境学会第 37 届年会记录(2003 年)。
- DOI:
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Y.Sekiguchi, Y.Kamagata, H.Harada: "Recent Advances in Methane Fermentation Technology"Current Opinion in Biotechnology. Vol.12 No.3. 277-282 (2001)
Y.Sekiguchi、Y.Kamagata、H.Harada:“甲烷发酵技术的最新进展”生物技术的当前观点。
- DOI:
- 发表时间:
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- 影响因子:0
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Chen, A.-C., Ohashi, A, Harada.H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)
Chen, A.-C.、Ohashi, A、Harada.H.:“模拟和实际垃圾填埋场样品中 H2/CO2 合成乙酸盐”环境技术。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Chen, A.-C., Ohashi, A., Harada, H.: "Acetate synthesis from H2/CO2 in simulated and actual landfill samples"Environmental Technology. 24. 435-444 (2003)
Chen, A.-C.、Ohashi, A.、Harada, H.:“模拟和实际垃圾填埋场样品中 H2/CO2 合成乙酸盐”环境技术。
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- 影响因子:0
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HARADA Hideki其他文献
HARADA Hideki的其他文献
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{{ truncateString('HARADA Hideki', 18)}}的其他基金
Development of a novel RNA detection method for understanding environmental microbial ecology
开发一种新的RNA检测方法来了解环境微生物生态学
- 批准号:
24656309 - 财政年份:2012
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
The international collaborating study of BIS monitoring system on rodents for the invention of new medicine
新药发明啮齿动物BIS监测系统国际合作研究
- 批准号:
24659707 - 财政年份:2012
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Construction of future forecast map and environmental policy scenario of water and the hygiene in Asian developing countries
亚洲发展中国家水与卫生未来预测图及环境政策情景构建
- 批准号:
20404002 - 财政年份:2008
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Ischemic postconditioning by electric or magnetic stimulation against the reperfusion injury after cerebral or spinal ischemia
电或磁刺激的缺血后处理对抗脑或脊髓缺血后的再灌注损伤
- 批准号:
20591821 - 财政年份:2008
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of energy-saving novel wastewater treatment systems
节能新型废水处理系统的开发
- 批准号:
19206056 - 财政年份:2007
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Data-base construction of Safe Water and Sanitation and Water-related Risk evaluation for Asian Developing Regions
亚洲发展中地区安全用水和环境卫生及水相关风险评估数据库建设
- 批准号:
18404002 - 财政年份:2006
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
International collaborative R&D on self-sustainable sewage treatment technology for alleviation of water-related risk in developing countries
国际合作研究
- 批准号:
16206054 - 财政年份:2004
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The ischemic tolerance induced by electromagnetic field exposure.
电磁场暴露引起的缺血耐受。
- 批准号:
13671625 - 财政年份:2001
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
ABSTRACTS OF RESEARCH PROJECT, GRANT-IN-AID FOR SCIENTIFIC RESEARCH
研究项目摘要、科学研究补助金
- 批准号:
12572043 - 财政年份:2000
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The ischemic tolerance induced by transcranial magnetic stimulation
经颅磁刺激诱导的缺血耐受
- 批准号:
11671531 - 财政年份:1999
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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