INTRINSIC ANTIMICROBIAL POLYPEPTIDE IN INFLAMMATROY BOWEL DISEASE

本研究利用生物学和免疫学技术分析了抗菌肽在IBD肠道和结肠中的表达。我们发现：1)和以前报道的小鼠肠道一样，从正常人肠道分离的隐窝可以在体外通过内毒素刺激分泌抗菌肽。2)分离的人隐窝的原位杂交和RT-PCR结果表明，抗菌活性是由Paneth细胞释放的人α-防御素(HD-5，HD-6)引起的。3)从IBD肠道分离的人隐窝也产生抗菌肽，与正常、非炎症肠道的抗菌肽一样。4)UC结肠中的Paneth细胞(化生Paneth细胞)也释放抗菌活性。5)人胃中的化生潘氏细胞也具有并释放有效杀死幽门螺杆菌的抗菌活性。这些结果已提交发表。

In this study we analyzed the expression of antimicrobial peptide in IBD intestine and colon by biological and immunological technique. We have found that 1) As well as a mouse intestine reported before, isolated crypts from normal human intestine could exocrine the antimicrobial peptide by the LPS stimulation, ex vivo. 2) In situ hybridization and RT-PCR of isolated human crypts revealed that antiomicrobial activity was caused by human a-defensin (HD-5, HD-6), released from Paneth cells. 3) Isolated human crypts from IBD intestine also produce the antimicrobial peptide as well as that of normal, noninflammed intestine. 4) Paneth cells in UC colon (metaplastic Paneth cell) also release the antimicrobial activity. 5) Metaplastic Paneth cells in human stomach also have and release antimicrobial activity that kills effectively H. Pylori.These results were submitted to publication.