Biological significance of Prolinerich antimicrobial peptide

点击翻译按钮获取中文摘要

We clarified several important points about syndecans and PR-39 through colaborations and discussions with Dr.Gallo as shown below.1.We made polyclonal antibody against PR-39 afer immunization of rabbits using N-terminal 15 aminoacids oigopeptides.2.We observed the band at the size of approximately 5 kDa as expected size of PR-39 mature protein afer immunoprecipitation of human leukocytes protein using PR-39 polyclonal antibody. However, we have not cloned the protein which might be human counterpart of pig PR-39.3.PR-39 has a possible function which can bind to Src homology 3 domain, becaus PR-39 has five repeats of proline rich motif that has been reported to bind SH3 domain as like a Sos protein which can bind to Grb2 protein. Recombinant SH3 domain of Src gene can actually bind to PR-39 oligopeptides by the study of immunopregipitation.4.PR-39 gene transfection into human hepatoma cells showed induction of syndecan-1 expression, suppression of invasive activity and cell morphological change. These results might be due to the binding ability of PR-39 into SH3 domain.5.PR-39 transfectans excreted PR-39 protein into conditioning medium.6.PR-39 gene transfection into ras transformants also showed morphological change and decrease of proliferation rate.