Active control of aggregation and delivery or nano-scale particle in the living system

主动控制生命系统中纳米级粒子的聚集和传递

• 批准号: 12450079
• 负责人: TANISHITA Kazuo
• 金额: $ 10.62万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450079/
• 关键词: blood substitute; liposome; microcirculation; biomechanics; Albumin micro-sphere; aggregation; 微粒子; 流動; 人工血小板; アルブミン粒子

In present work, we studied the ability of platelet substitute, rGPIbα-AMS, in the process of hemostasis. The physiology of the platelet in hemostasis has been extensrvely evaluated, and the role of glycoproteins on platelet membrane surface has defined. It is also known that the fluid mechanical behavior of platelets makes the thrombus formation more effective. It is important to know how the platelet substitute could form the thrombus with or without native platelet. And to this end, it is helpful to study the motion of each substitute particles under flow condition.In this study we directly observed the motion of artificial platelet toward ligand coating surface using the rectangular channel flow chamber, and the High Speed Camera with Image Intensifier. Then concentration profiles along a radius and particle velocity vectors were measured.At high shear rate, the concentration profiled of large rGPIbα-AMS occurred large wall-near excess. It might accelerate the interaction between particles and wall. Once particles adhered to the surface, the trajectories flowing near the wall became unsteady. To assess the deviation, we calculate the drift angle Φ, which is the angle of vector from axial direction, the angle was the highest near the wall at low shear rate/ because adhering particle change the geometry of the wall. Such phenomenon might occur in thrombus formation, and it seems favorable for artificial platelet.

本文研究了血小板代用品rGPIbα-AMS在止血过程中的作用。血小板在止血中的生理作用已被广泛地评价，血小板膜表面糖蛋白的作用已被确定。还已知血小板的流体机械行为使得血栓形成更有效。了解血小板替代物在有无自体血小板的情况下如何形成血栓是非常重要的。本研究利用矩形通道流动室和高速摄像机结合图像增强器，直接观察了人工血小板向配体涂层表面的运动。在高剪切速率下，大颗粒rGPIbα-AMS的浓度分布出现了较大的近壁过量。这可能会加速颗粒与壁面之间的相互作用。一旦颗粒附着在表面上，在壁面附近流动的轨迹变得不稳定。为了评估偏差，我们计算漂移角Φ，其是矢量与轴向方向的角度，该角度在低剪切速率下在壁附近最高，因为粘附颗粒改变了壁的几何形状。这种现象可能发生在血栓形成过程中，对人工血小板的形成是有利的。

项目成果

谷下一夫他: "矩形管モデルにおける人工血小板の壁近傍における挙動"第40回日本ME学会大会論文集. 第39巻特別号. 665 (2001)

Kazuo Tanishita 等人：“矩形管模型中壁附近人工血小板的行为”第 40 届日本 ME 学会会议记录第 39 卷特刊（2001 年）。

Tanishita K., Deguchi, T., Tsuji, T., Nishida, T., Ikeda, Y.: "Motion of artificial platelet in the model small artiry"ICTAM. 98 (2000)

Tanishita K.、Deguchi, T.、Tsuji, T.、Nishida, T.、Ikeda, Y.：“人造血小板在模型小动脉中的运动”ICTAM。

谷下一夫他: "流れ負荷による内皮細胞の形態再構築過程における細胞表面のせん断応力分布"日本機械学会論文集. (掲載予定). (2002)

Kazuo Tanishita 等人：“由于流动载荷导致的内皮细胞形态重建过程中细胞表面的剪切应力分布”，日本机械工程师学会会刊（待出版）。

T. Tsuji, T.T. Wada, Nishiya, Y. Ikeda, K. Tanishita: "Flow distribution and motion of artificial platelets in arteriole model"The 11th JSME Autumn Bioengineering Conference and Seminar. 107-108 (2000)

T. Tsuji、T.T. Wada、Nishiya、Y. Ikeda、K. Tanishita：“人工血小板在微动脉模型中的流动分布和运动”第11届JSME秋季生物工程会议暨研讨会。

T. Tsuji, T.T. Wada, S. Takeoka, Y. Ikeda, K. Tanishita: "Behavior of artificial platelets in a rectangular tube model"Proc. The 40th conference, JJME. Vol. 39. 665 (2001)

T. Tsuji、T.T. Wada、S. Takeoka、Y. Ikeda、K. Tanishita：“人造血小板在矩形管模型中的行为”Proc。