Developent of cascade photo-bioreactor for the fixation of carbon dioxide

我们设计了具有反射器的新型反应器以减少光源的能量损失，并且提出了将波长从绿色区域转移到红色区域的荧光反射器以用于有效的光合作用。在本研究中，我们评估了使用银反射器（5-参考）的生物反应器的效果。和荧光反射器（F-Ref.）在聚对苯二甲酸乙二醇酯（PET）基片上溅射银，并覆盖PET膜作为S-Ref（反射率94%）。作为F-参比（59%反射率），将荧光染料（Lumogen F-Red 300，BASF Japan Ltd.）使用黑色帘作为对照。采用同轴双管光生物反应器进行测量，该反应器内部含有光源，外部含有藻类培养物（藻类悬浮液的光路为2.3 mm），并覆盖这些反射器，灌注培养绿色藻类小球藻IAM 关于我们 C-27。在预培养3天后，将藻类培养物灌注在光生物反应器中。氧的产生被测量为光合速率。在40 W/m ~ 2的照射下，尽管藻细胞浓度增加了5倍（=0.344g/l），但O_2的产生率仅增加了1.7倍。但S-Ref.在对照（细胞浓度= 0.344g/l）中提高了2.3倍O2生产率，在F-Ref中提高了2.0倍，因此这些反射器，特别是S-Ref.更有效地利用能量。但F-Ref.比S-Ref.具有上级优势，单位反射光能的O2产生率是S-Ref.的1.7倍，其中F-Ref.的光谱移动对光合作用是有效的。虽然S-Ref.的生长曲线与S-Ref.的生长曲线相似，16小时后细胞的组成不同：母细胞在S-Ref.中占优势，F-Ref中子细胞占优势，说明红光加速了细胞分裂，而小的子细胞（高表面积/体积比）也改善了生物反应器中的光照条件。少

we designed the new reactor with reflectors to reduce the energy loss of the light source and propose the fluorescent reflector that shifts wavelength from green region to red region for effective photosynthesis. In present study, we evaluated the effect of a bioreactor using silver reflector (5-Ref.) and fluorescent reflector (F-Ref.) on photosynthetic rates, and investigated the influence of spectral shifting by fluorescent reflector on algal cells.Base polyethylene terephthalate (PET) sheet was sputtered with silver and covered with PET film as a S-Ref. (94%Reflectivity). As a F-Ref. (59%Reflectivity), fluorescent dye (Lumogen F-Red 300, BASF Japan Ltd.) was coated on the S-Ref. A black curtain was used as control. Coaxial double tubular photobioreactors were used for measurements, which contain light sources inside and algal culture outside (light path of algal suspension is 2.3 mm) and were covered with these reflectors with perfusing culture of green algae Chlorella vulgaris IAM … More C-27. After preculture of 3 days, the algal culture were perfused in the photobioreactors. Oxygen production was measured as photosynthetic rates. As influence of spectral shifting by fluorescent reflector on algal cells, cell concentration, cell volume and absorption spectrum were measured during 8 days.At 40W/m2 irradiation in control, in spite of five-fold increase of cell concentration (=0.344g/l), O2 production rate rose only 1.7-fold. But S-Ref. rose 2.3-fold 02 production rate in control (cell concentration=O.344 g/l), and 2.0-fold in F-Ref. So these reflectors, especially S-Ref. Utilizes energy more efficiently. However F-Ref. was superior to S-Ref. with 1.7 times in O2 production rate per reflected light energy, in which the spectral shifting by F-Ref. is effective for photosynthesis. Although the growth curve of S-Ref. is similar to one of S-Ref., the composition of the cells after 16 hrs is different : mother cells are dominant in S-Ref., and daughter cells are dominant in F-Ref. These result imply that the red light accelerated the cell fission, and the small daughter cells (high surface / volume ratio) also improve the lighting condition in the bioreactor. Less