Developent of cascade photo-bioreactor for the fixation of carbon dioxide

我们设计了带有反射器的新型反应器，以减少光源的能量损失，并提出了将波长从绿区转换到红区的荧光反射器，以实现有效的光合作用。在本研究中，我们评估了使用银反射器的生物反应器的效果(参考文献5)。和荧光反射器(F-Ref.)研究了荧光反射器的光谱漂移对藻类细胞的影响。在聚对苯二甲酸乙二醇酯基片上溅射银并在聚酯膜上覆盖，作为S参考文献。(94%的反射率)。作为F-Ref。(59%反射率)、荧光染料(Lumogen F-Red 300，巴斯夫日本有限公司)被涂在了S的裁判身上。用黑色窗帘作为对照。测量采用同轴双管式光生物反应器，内有光源，外有藻类培养(藻类悬浮光路为2.3 mm)，并覆盖有绿藻灌流培养的普通小球藻IAM…的反射镜更多的C-27。预培养3d后，在光生物反应器中灌流藻类培养物。用光合作用速率来测量产氧量。由于荧光反射器的光谱漂移对藻细胞的影响，在8d的时间里测量了细胞浓度、细胞体积和吸收光谱。在40W/m2的照射下，对照的细胞浓度增加了5倍(=0.344g/L)，产氧率仅增加了1.7倍。但S--参考文献。对照(菌体浓度=0.344g/L)的产生率提高2.3倍，F-Ref提高2.0倍。所以这些反光镜，尤其是S裁判。更有效地利用能源。然而，F-Ref.优于S-裁判。与1.7倍的产氧率每反射光能，其中光谱漂移F-Ref。对光合作用是有效的。虽然S的生长曲线。与S文献相似，16h后细胞组成不同：S文献以母细胞为主，F文献以子细胞为主。这些结果表明，红光加速了细胞的分裂，较小的子代细胞(高表面积/体积比)也改善了生物反应器中的光照条件。较少

we designed the new reactor with reflectors to reduce the energy loss of the light source and propose the fluorescent reflector that shifts wavelength from green region to red region for effective photosynthesis. In present study, we evaluated the effect of a bioreactor using silver reflector (5-Ref.) and fluorescent reflector (F-Ref.) on photosynthetic rates, and investigated the influence of spectral shifting by fluorescent reflector on algal cells.Base polyethylene terephthalate (PET) sheet was sputtered with silver and covered with PET film as a S-Ref. (94%Reflectivity). As a F-Ref. (59%Reflectivity), fluorescent dye (Lumogen F-Red 300, BASF Japan Ltd.) was coated on the S-Ref. A black curtain was used as control. Coaxial double tubular photobioreactors were used for measurements, which contain light sources inside and algal culture outside (light path of algal suspension is 2.3 mm) and were covered with these reflectors with perfusing culture of green algae Chlorella vulgaris IAM … More C-27. After preculture of 3 days, the algal culture were perfused in the photobioreactors. Oxygen production was measured as photosynthetic rates. As influence of spectral shifting by fluorescent reflector on algal cells, cell concentration, cell volume and absorption spectrum were measured during 8 days.At 40W/m2 irradiation in control, in spite of five-fold increase of cell concentration (=0.344g/l), O2 production rate rose only 1.7-fold. But S-Ref. rose 2.3-fold 02 production rate in control (cell concentration=O.344 g/l), and 2.0-fold in F-Ref. So these reflectors, especially S-Ref. Utilizes energy more efficiently. However F-Ref. was superior to S-Ref. with 1.7 times in O2 production rate per reflected light energy, in which the spectral shifting by F-Ref. is effective for photosynthesis. Although the growth curve of S-Ref. is similar to one of S-Ref., the composition of the cells after 16 hrs is different : mother cells are dominant in S-Ref., and daughter cells are dominant in F-Ref. These result imply that the red light accelerated the cell fission, and the small daughter cells (high surface / volume ratio) also improve the lighting condition in the bioreactor. Less