Creation and Bio-Functional Material Engineering of Biomimetic Aqueous Adhesion System, Using Adhesive Proteins.

使用粘附蛋白的仿生水粘附系统的创建和生物功能材料工程。

• 批准号: 12450330
• 负责人: YAMAMOTO Hiroyuki
• 金额: $ 8.96万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450330/
• 关键词: Marine Adhesive Protein; Protein Synthesis; Crosslinking and Insolubilization; Wettability and Adhesion Property; Interfacial Adhesion Process; Bio-Adhesive; Biomimetic Composite Material; Biotechnology; 濡れ・表面特性; 水不溶化機構; 濡れ特性; 分子配列設計; 生物接着工学

1.Exploring New marine Adhesive Protein and its Molecular StructureThe adhesive protein of the green mussel Perna viridis was firstly purified. The molecular structure of the Perna viridis foot protein-1 (Pvfp-1) was characterized as a novel glycosylated protein. The other proteins, Pvfp-2, -3, -4, -5, -6, -7, -and -8 were also found as the byssus precursor protein, and some of them functions as the adhesives to male durable marine holdfast.2.Reinforced Fiber material Inspired from the Byssoginous System of the Matrine MusselThe molecular mechanism involved in the byssogenous system of the marine mussels inspires the mew technology for creating a hybrid material in aqueous media. In a series of our studies to create the biomimetic hybrid fibers, capsules, hydrogels, and adhesives, we focused on the quinone-coupling chemistry, which occurs along with the byssus formation. The novel polysaccharide and poly (amino acid) derivatives were designed and synthesized. They have the grafted peptide side chan as the enzyme-mediated cross-linker, allowing the mechanical reinforcement of the hybrid materials.3.Dynamic Wettability of the Adhesive Proteins and Related Polymeric CompoundsTo discuss the surface adsorption of the marine adhesive protein, the dynamic contact angle measurement is very informative due to its accessibility to the wetting phenomenon in real-time. Also, the direct measurement of the wetting forces afford the amount of the protein adsorption along with the wetting process.

1.探索新型海洋黏附蛋白及其分子结构首次对翡翠贻贝黏附蛋白进行了纯化。翡翠贻贝足部蛋白-1(Pvfp-1)是一种新的糖基化蛋白。Pvfp-2、Pvfp-3、Pvfp-4、Pvfp-5、Pvfp-5、Pvfp-6、Pvfp-7和Pvfp-8也是Besssus前体蛋白，它们中的一些可以作为雄性耐久船舱的粘合剂。2.增强纤维材料源于苦参碱贻贝的双生系统海洋贻贝的共生系统所涉及的分子机制启发了在水介质中创造杂交材料的新技术。在我们创造仿生杂化纤维、胶囊、水凝胶和粘合剂的一系列研究中，我们专注于苯醌偶联化学，它伴随着体节的形成而发生。设计合成了新型多糖类和多(氨基酸)类化合物。3.粘着蛋白及其相关聚合物的动态润湿性为了探讨海洋粘着蛋白的表面吸附，动态接触角的测量具有很强的信息量，因为它可以实时地接触到润湿现象。此外，润湿力的直接测量提供了随着润湿过程而产生的蛋白质的吸附量。

项目成果

Y.Hirata, Y.Sano, M.Aoki, H.Shohji, S.Katoh, J.Abe, H.Hitsukuti, H.Yamamoto: "Small-angle X-ray scattering studies of moderately concentrated dextran solution"Carbohydrate Polymers. 53. 331-335 (2003)

Y.Hirata、Y.Sano、M.Aoki、H.Shohji、S.Katoh、J.Abe、H.Hitsukuti、H.Yamamoto：“中等浓度葡聚糖溶液的小角 X 射线散射研究”碳水化合物聚合物。

Y.Hirata: "Small-angle X-ray scattering studies of moderately concentrated dextran solution"Carbohydrate Polymers. 53. 331-335 (2003)

Y.Hirata：“中等浓度葡聚糖溶液的小角 X 射线散射研究”碳水化合物聚合物。

K.Ohkawa: "Photoresponsive peptide and polypeptide systems 15 : Synthesis of photo-crosslinkable poly(amino acid)s by watery process and its application as a reinforcement for polyion complex fibers"J.Mater.Sci.. 38(15). 3191-3197 (2003)

K.Ohkawa：“光响应肽和多肽系统 15：通过水法合成可光交联的聚（氨基酸）及其作为聚离子复合纤维增强材料的应用”J.Mater.Sci. 38（15）。

大川浩作: "ポリイオンコンプレクス型天然素材繊維"繊維と工業. 60(1). 15-20 (2004)

Kosaku Okawa：“聚离子复合型天然纤维”纺织工业60（1）（2004）。

A.Nishida: "Green mussel Perna viridis L. : Attachment behaviour and preparation of antifouling surfaces"Biomol.Eng.. 20(4-6). 381-387 (2003)

A.Nishida：“绿贻贝Perna viridis L.：附着行为和防污表面的制备”Biomol.Eng.. 20(4-6)。