Analysis of antibody-dependent cellular virus inhibition(ADCVI) against SIV-infected cells

艾滋病病毒中和抗体在体内急性期非无菌病毒复制控制中起关键作用。为了评估抗体介导的先天抑制对病毒感染细胞在这一过程中的作用，建立了体外抗体依赖性细胞病毒抑制（ADCVI）测定系统。抗体的病毒结合度与ADCVI相关，而与中和活性无关。为了直接估计ADCVI的贡献，我们对siv感染的猕猴被动免疫抗siv抗体，选择性地抑制ADCVI但不中和活性，这不会导致明显的病毒血症减少或在病毒基因组序列中施加选择压力。综上所述，ADCVI在体内中和基于抗体的非无菌SIV控制中的作用是有限的。

AIDS virus neutralizing antibodies can play a critical role in acute phase non-sterile virus replication control in vivo. To assess the contribution of antibody-mediated innate inhibitory effects on virus-infected cells within this process, an in vitro antibody-dependent cellular virus inhibition(ADCVI) assay system was established. Virus-binding avidity of antibodies correlated with ADCVI while they did not correlate with neutralizing activity. For direct estimation of ADCVI contribution, anti-SIV antibodies selectively withholding ADCVI but not neutralizing activity were passively immunized against SIV-infected macaques, which did not result in palpable viremia reduction or exertion of selective pressure in viral genome sequences. Taken together, the role of ADCVI in neutralizing antibody-based non-sterile SIV control in vivo was suggested to be limited.