Artificial liver support based on the regenerative medicine

基于再生医学的人工肝支持

• 批准号: 12470233
• 负责人: KASAI Shinichi
• 金额: $ 3.39万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470233/
• 关键词: hepatic failure; hepatocytes; artificial liver; bone marrow cells; hepatocyte transplantation; regenerative medicine; ハイブリッド人工肝臓; 肝膵相関; 肝細胞分化; 肝膵移植; 胎児肝、膵; ハイブリッド型人工肝臓; 遊離肝細胞; 膵ラ氏島; 胎児肝; 胎児膵; ゲル包埋肝細胞

Studies of hybrid artificial liver due to tissue engineering of hepatocytes and islets.The liver is an organ with various complex functions to maintain the metabolism of the body. Hepatic failure sometimes can be irreversible and its prognosis is then poor. Two major approaches are there for the treatment of hepatic failure. One is the use of a hybrid artificial liver support system and the other is liver transplantation. In this study, new approach was investigated in the points of tissue engineering. Adult and fatous hepatocytes, islet cells were examined as cell sources of hybrid artificial liver and cell transplantation, and also examined the transformation of born marrow cells to hepatocytes and those cryopreservation.The coculture and cotransplantation of hepatocytes and isolated islets revealed the hepatic function such as metabolic and detoxification functions. Transformation of bone marrow cells to hepatocytes were observed in transplantation experiments in rats.

肝细胞和胰岛组织工程杂交人工肝的研究肝脏是维持机体代谢的具有多种复杂功能的器官。肝衰竭有时可能是不可逆的，其预后很差。肝衰竭的治疗主要有两种方法。一种是使用混合型人工肝支持系统，另一种是肝移植。本研究从组织工程的角度探讨了新的方法。研究了成人肝细胞、脂肪肝细胞、胰岛细胞作为混合型人工肝和细胞移植的细胞来源，以及骨髓细胞向肝细胞的转化和冻存，肝细胞与胰岛细胞的共培养和共移植，揭示了肝细胞的代谢和解毒功能。在大鼠移植实验中观察到骨髓细胞向肝细胞的转化。

项目成果

Kasai S.: "Treatment of hepatic failure by hepatocytes transplantation"Tanpakushitu, Kakusan, Koso. 45(13). 2301-2306 (2000)

Kasai S.：“肝细胞移植治疗肝衰竭”Tanpakushitu，Kakusan，Koso。

葛西眞一: "人工肝臓-肝細胞浮遊型人工肝臓"外科. 63・5. 522-527 (2001)

葛西真一：“人工肝脏-肝细胞漂浮人工肝脏”63・527（2001）。

葛西眞一: "低温環境利用技術ハンドブック(肝細胞保存)"森北出版株式会社. 510 (2001)

葛西真一：《低温环境利用技术手册（肝细胞保存）》森北出版510（2001）

葛西眞一: "Annual Review 消化器 2000 (肝細胞移植)"中外医学社. 378 (2000)

Shinichi Kasai：“年度回顾胃肠病学 2000（肝细胞移植）”Chugai Igakusha 378（2000）。

葛西 眞一: "肝細胞移植による肝不全の治療"蛋白質・核酸・酵素. 45(13). 2301-2306 (2000)

Shinichi Kasai：“肝细胞移植治疗肝衰竭”蛋白质、核酸和酶45（13）（2000）。