Studies of hepatic reconstruction for metabolic liver support.

用于代谢性肝脏支持的肝脏重建研究。

基本信息

  • 批准号:
    02670530
  • 负责人:
  • 金额:
    $ 1.47万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1991
  • 项目状态:
    已结题

项目摘要

The liver is an organ with various complex functions to maintain the metabolism of the body. Acute or chronic impairment of the function sometimes can be irreversible and its prognosis is then poor. There have been two major approaches investigated for the treatment of hepatic failure. One is the use of an artificial liver support system(ALS)and the other is liver transplantation. Method of ALS like hemoperfusion, hemodialysis or filtration, and plasmapheresis have already been adopted to treat patients. However the survival rate has not so improved as expected, probably due to the lack of metabolic support capacity. This study aimed to develop a new hybrid artificial liver due to the reconstruction of hepatic tissue using isolated hepatocytes.Isolated hepatocytes were obtained by colagenase digestion method. First experiments were done to reconstruct hepatic tissue in the spleen for functional support of chronic liver impairment. Hepatocytes were transplanted into the rat spleen and hepatic tissue mass was reconstruct after over one year. In order to speed up the gmass rowth, the serum after 70% hepatectomy and AAF treatment which is the astimulating factor of liver regeneration and epidermal growth factor, were applied in traperitoneally. The growth effects were observed but still it is not sufficient. Second experiments were designed to develop a new metabolic support system. Some kinds of basic culture medium and some additives were investigated of their availability for the improvement of metabolic functions of eretrapped hepatocytes with Ca-alinate. Metabolic function of entrapped hepatocytes depended on the quality of basic culture medium more than additives investigated in this study.
肝脏是一个具有各种复杂功能的器官,以维持机体的新陈代谢。急性或慢性功能损害有时可能是不可逆的,其预后很差。已经研究了两种用于治疗肝衰竭的主要方法。一种是使用人工肝支持系统(ALS),另一种是肝移植。ALS的治疗方法如血液灌流、血液透析或滤过、血浆置换等已被用于治疗患者。然而,存活率并没有像预期的那样提高,可能是由于缺乏代谢支持能力。本研究旨在利用胶原酶消化法获得肝细胞,通过分离肝细胞重建肝组织,构建一种新型的混合型人工肝。本研究首先进行了脾内重建肝组织对慢性肝损伤的功能支持实验。将肝细胞移植到大鼠脾脏内,一年多后重建肝组织块。为加速肿瘤生长,腹腔注射70%肝切除术后血清及肝再生刺激因子和表皮生长因子AAF治疗。观察到了生长效应,但仍然不够。第二个实验设计开发一种新的代谢支持系统。本文研究了几种基本培养基和几种添加剂对丙氨酸钙诱导的肝细胞代谢功能的改善作用。包埋肝细胞的代谢功能更依赖于基础培养基的质量,而不是本研究中所研究的添加剂。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
葛西 眞一: "ゲル固定化肝細胞の高機能化に関する基礎的研究" 人工臓器. 20. 167-172 (1991)
Shinichi Kasai:“凝胶固定肝细胞的高功能性基础研究”Artificial Organs。 20. 167-172 (1991)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kasai, S.: "A metabolic support device for a hybrid artificial liver." Artificial Organs. 14. 260-261 (1990)
Kasai, S.:“混合型人工肝的代谢支持装置。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
葛西 眞一: "ゲル固定化肝細胞の高機能化に関する基礎的研究" 人工臓器. 20. (1991)
Shinichi Kasai:“提高凝胶固定肝细胞功能的基础研究”人工器官20。(1991)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
葛西 眞一: "肝細胞移植およびHybrid Artificial Liver" 集中治療. 2. 443-450 (1990)
Shinichi Kasai:“肝细胞移植和混合人工肝”重症监护 2. 443-450 (1990)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kasai, S.: "Hybrid artificial liver." JSAIO. 19(6). 443-450 (1990)
Kasai, S.:“混合人工肝。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KASAI Shinichi其他文献

KASAI Shinichi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KASAI Shinichi', 18)}}的其他基金

A study of prehomogeneous vector spaces
预齐次向量空间的研究
  • 批准号:
    13640027
  • 财政年份:
    2001
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Artificial liver support based on the regenerative medicine
基于再生医学的人工肝支持
  • 批准号:
    12470233
  • 财政年份:
    2000
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
CROSS TALK BETWEEN BIOLOGICAL TRIPLET SYSTEMS (NEURO-ENDCRINE-IMMUNE) DURING LIVER REGENERATION
肝脏再生过程中生物三联体系统(神经内分泌免疫)之间的交互作用
  • 批准号:
    07407030
  • 财政年份:
    1995
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
STUDIES ON HYBRID ARTIFICIAL LIVERS IN POINTS OF THE TECHNOLOGY FOR ORGAN RECONSTRUCTION
混合型人工肝从器官重建技术角度的研究
  • 批准号:
    04454307
  • 财政年份:
    1992
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on Biomimetic Artificial Liver
仿生人工肝的研究
  • 批准号:
    63570585
  • 财政年份:
    1988
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Pathophysiological studies of hypofibronectineamia and superoxide radical in multiple organ failure (MOF)
多器官衰竭(MOF)中低纤维连接血症和超氧自由基的病理生理学研究
  • 批准号:
    61570599
  • 财政年份:
    1986
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Development of the innovative hybrid artificial liver with establishment of the hepatocyte strain from human iPS cells
通过从人类 iPS 细胞中建立肝细胞株来开发创新型混合人工肝
  • 批准号:
    25462093
  • 财政年份:
    2013
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a Hybrid Artificial Liver Containing ES Cell-derived Hepatic Cells and Its Evaluation as an Alternative to Liver Transplantation
含有 ES 细胞来源的肝细胞的混合人工肝的开发及其作为肝移植替代方案的评估
  • 批准号:
    19360375
  • 财政年份:
    2007
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a hybrid artificial liver with hepatocytes derived embryonic stem cell for chronic liver failure patients
为慢性肝衰竭患者开发具有肝细胞来源的胚胎干细胞的混合人工肝
  • 批准号:
    16206079
  • 财政年份:
    2004
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of a Scaffold with Optimal Structure and Application to a Hybrid Artificial Liver for Chronic Liver Failure Patients
最佳结构支架的研制及其在慢性肝衰竭患者混合型人工肝中的应用
  • 批准号:
    15360443
  • 财政年份:
    2003
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a hybrid artificial liver using small hepatocytes with high proliferative activity
使用具有高增殖活性的小肝细胞开发混合人工肝
  • 批准号:
    14370344
  • 财政年份:
    2002
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a Hybrid Artificial Liver for Treatment of Chronic Liver Failure Patients
开发用于治疗慢性肝衰竭患者的混合人工肝
  • 批准号:
    14205119
  • 财政年份:
    2002
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of a Hybrid Artificial Liver for Treatment of Acute and Chronic Liver Failure Patients
开发用于治疗急性和慢性肝衰竭患者的混合人工肝
  • 批准号:
    12305053
  • 财政年份:
    2000
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Construction of Hybrid Artificial Liver with Immortalized Human Hepatocytes
永生化人肝细胞混合人工肝的构建
  • 批准号:
    12671175
  • 财政年份:
    2000
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a hybrid artificial liver using artificial blood
使用人造血液开发混合型人工肝
  • 批准号:
    10470249
  • 财政年份:
    1998
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Development of Hybrid Artificial Liver for Clinical Treatment of Fulminant Hepatic Failure Patient
混合型人工肝的开发用于临床治疗暴发性肝衰竭患者
  • 批准号:
    10305062
  • 财政年份:
    1998
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了