Molecular biological Research on mechanisms of refinoic acid for dermal wound healing and treatment of hyper pigmentation

维甲酸促进真皮伤口愈合和治疗色素沉着过度机制的分子生物学研究

• 批准号: 12470374
• 负责人: YOSHIMURA Kotaro
• 金额: $ 9.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470374/
• 关键词: retinoic acid; HB-EGF; melanin; melanocyte; hydroguinone; retinol; retinal; レチノイド; ハイドロコーチゾン; ケラチノサイト

The results suggested that RA neither have direct inhibitory effects on melanogenesis of melanocytes, nor influence the cell-cell interactions between melanocytes, keratinocytes and fibroblasts, such as paracrine actions with regard to melanin productions. The role of RA in bleaching treatments appears to be other specific actions, such as promotion of keratinocytes proliferation and acceleration of epidermal turnover. The results also revealed that HB-EGF mRNA expression was elevated dose-dependently with atRA, most at 12 hours. This elevation was more prominent in keratinocytes of confluent state than those of subconfluent state, suggesting differentiated keratinocytes are more subject to be stimulated by atRA with regard to HB-EGF expression than proliferating keratinocytes. Ral and Rol was found to upregulate HB-EGF when used at 100 μM to 1mM, to a similar extent of atRA at 1 to 10 μM. The capacity of retinoids to upregulate HB-EGF may be an important index to develop and seek an ideal synthetic retinoid, which has maximum benefits and minimal side effects.

结果表明，RA既不对黑素细胞的黑色素生成有直接的抑制作用，也没有影响黑素细胞，角质形成细胞和成纤维细胞之间的细胞细胞相互作用，例如在黑色素生产方面的旁分泌作用。 RA在漂白处理中的作用似乎是其他特定的作用，例如促进角质形成细胞增殖和表皮转让的加速。结果还表明，HB-EGF mRNA表达用ATRA依赖性剂量升高，大部分在12小时时。在汇合态的角质形成细胞中，这种升高比亚征态状态的升高更为突出，这表明与增殖的角质形成细胞相比，在HB-EGF表达方面，ATRA刺激了分化的角质形成细胞。发现在100μm至1mm时使用RAL和ROL可以上调HB-EGF，在1至10μm处的ATRA程度相似。类视黄素上调HB-EGF的能力可能是开发和寻求理想合成性类维生素的重要指数，该指数具有最大的益处和最小的副作用。

项目成果

Yoshimura K, et al: "Experience of a strong bleaching treatment"Plastic Reconstructive Surgery. 105. 1097-1110 (2000)

Yoshimura K等人：“强力漂白治疗的经验”整形重建外科。

K.Yoshimura: "The Effect of Two Episodes of-"Plast.Reconstr.Surg.. 109. 212-219 (2002)

K.Yoshimura：“两集的效果-”Plast.Reconstr.Surg.. 109. 212-219 (2002)

K.Yoshimura: "The Effects of Two Episodes of 〜"Plast Reconstr. Surg. 109. 212-219 (2002)

K.Yoshimura：“〜”整形外科手术的两集的影响。 109. 212-219 (2002)

Y.Kitano: "Wound Healing of genetically diabetic"Arch Dermatol Res. 293. 515-521 (2001)

Y.Kitano：“遗传性糖尿病的伤口愈合”Arch Dermatol Res。

Gonda K, et al: "Heterotopic occifioation of degenerating rat"Bone & Mineral Reseach. 15. 1056-1065 (2000)

Gonda K 等人：“退化大鼠的异位结合”骨