权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on tissue and vascular regeneration using adipose-derived stem cells.

利用脂肪干细胞进行组织和血管再生的研究。

基本信息

批准号：
16390507
负责人：
YOSHIMURA Kotaro
金额：
$ 8.9万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Liposuction aspirates (primarily saline solution, blood, and adipose tissue fragments) separate into fatty and fluid portions. Cells isolated from the fatty portion are termed processed lipoaspirate (PTA) cells and contain adipose-derived adherent stromal cells (ASCs). Here we define cells isolated from the fluid portion of liposuction aspirates as liposuction aspirate fluid (LAF) cells. Stromal vascular fractions (SVF) were isolated separately from both portions and characterized under cultured and non-cultured conditions. A comparable number of LAF and PLA cells were freshly isolated, but fewer LAF cells were adherent. CD34+CD45- cells from fresh LAF isolates were expanded by adherent culture, suggesting that LAF cells contain ASCs. Although freshly isolated PLA and LAF cells have distinct cell surface marker profiles, adherent PLA and LAF cells have quite similar characteristics with regard to growth kinetics, morphology, capacity for differentiation, and surface marker profiles. After plating, both PLA and LAF cells showed significant increased expression of CD29, CD44, CD49d, CD73, CD90, CD105,and CD151 and decreased expression of CD31 and CD45. Multicolor FACS analysis revealed that SVF are composed of heterogeneous cell populations including blood-derived cells (CD45+), ASCs (CD31-CD34+CD45-CD90+CD105-CD146-), endothelial (progenitor) cells (CD31+CD34+CD45-CD90+CD105lowCD146+), pericytes (CD31-CD34-CD45-CD90+CD105-CD146+), and other cells. After plating, ASCs showed a dramatic increase in CD105 expression. Although some adherent ASCs lost CD34 expression with increasing culture time, our culture method maintained CD34 expression in ASCs for at least 10-20 weeks. These results suggest that liposuction-derived cells may be useful and valuable for cell-based therapies.

吸脂吸出物（主要是盐溶液、血液和脂肪组织碎片）分离成脂肪和液体部分。从脂肪部分分离的细胞被称为经处理的脂肪抽吸物（PTA）细胞，并且含有脂肪来源的贴壁基质细胞（ASC）。在这里，我们将从吸脂抽吸物的液体部分分离的细胞定义为吸脂抽吸液（LAF）细胞。分别从两个部分分离基质血管组分（SVF），并在培养和非培养条件下进行表征。相当数量的LAF和PLA细胞新鲜分离，但较少的LAF细胞贴壁。通过贴壁培养扩增来自新鲜LAF分离物的CD 34 + CD 45-细胞，表明LAF细胞含有ASC。虽然新鲜分离的PLA和LAF细胞具有不同的细胞表面标志物谱，但贴壁PLA和LAF细胞在生长动力学、形态学、分化能力和表面标志物谱方面具有非常相似的特征。接种后，PLA和LAF细胞均显示CD 29、CD 44、CD 49 d、CD 73、CD 90、CD 105和CD 151的表达显著增加，而CD 31和CD 45的表达降低。多色FACS分析显示，SVF由异质细胞群组成，包括血液来源的细胞（CD 45+）、ASC（CD 31-CD 34 + CD 45-CD 90 + CD 105-CD 146-）、内皮（祖）细胞（CD 31 + CD 34 + CD 45-CD 90 + CD 105-CD 146+）、周细胞（CD 31-CD 34-CD 45-CD 90 + CD 105-CD 146+）和其他细胞。接种后，ASC显示出CD 105表达的显著增加。虽然一些贴壁ASCs随着培养时间的增加而失去CD 34表达，但我们的培养方法在ASCs中维持CD 34表达至少10-20周。这些结果表明，吸脂衍生的细胞可能是有用的和有价值的细胞为基础的治疗。