Functions of adipose-derived stem cells in remodeling of tissue and vasculature

脂肪干细胞在组织和脉管系统重塑中的功能

• 批准号: 21390477
• 负责人: YOSHIMURA Kotaro
• 金额: $ 11.4万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390477/
• 关键词: 脂肪由来幹細胞; 血管内皮細胞; 虚血; 創傷治癒; 血管新生; 組織傷害因子; 糖尿病; 脂肪幹細胞

Many features of adipose stem/progenitor cells, such as physiological functions and localization, have been clarified in the past decade. Adipose tissue turns over very slowly and its perivascular progenitor cells differentiate into new adipocytes, replacing dead adipocytes. A number of clinical trials using freshly isolated or cultured adipose-derived stromal cells(ASCs) containing adipose progenitor/stem cells are ongoing in more than ten countries. Therapeutic use of adipose stem/progenitor cells has been shown to promote angiogenesis and adipose tissue regeneration. Identification of adipocyte releasing factors upon apoptosis/necrosis would be a break-through to step up to the next stage for adipose tissue regeneration. Activation of precursors in perichondrium and periosteum shows a dramatic neogenesis by only a simple injection and an ideal example of in situ tissue engineering. The "hit and catch" strategy using a mobilizer of bone-marrow stem/progenitor cells and a catcher(attractants) to lead the cells to proper homing into the target tissue may be our future direction of promote manipulation of stem cells. Careful design of microenvironment activating ASCs, cell delivery protocol to avoid unexpected behavior and induce maximal potential of ASCs, and selection of target diseases, will be critical to the success of clinical applications.

脂肪干/祖细胞的生理功能和定位等许多特性在过去的十年中已被阐明。脂肪组织翻转非常缓慢，其血管周围的祖细胞分化成新的脂肪细胞，取代死亡的脂肪细胞。在十多个国家正在进行使用新鲜分离或培养的含有脂肪祖细胞/干细胞的脂肪源性基质细胞（ASC）的许多临床试验。脂肪干细胞/祖细胞的治疗用途已显示出促进血管生成和脂肪组织再生。在细胞凋亡/坏死时识别脂肪细胞释放因子将是迈向脂肪组织再生的下一阶段的突破。软骨膜和骨膜中的前体细胞的激活显示了仅通过简单的注射即可实现的戏剧性新生，是原位组织工程的理想实例。利用骨髓干/祖细胞的动员剂和捕获剂（吸引剂）引导细胞正确归巢到靶组织的“击中和捕获”策略可能是我们促进干细胞操作的未来方向。精心设计激活ASCs的微环境，避免意外行为并诱导ASCs最大潜力的细胞递送方案，以及靶向疾病的选择，将是临床应用成功的关键。

项目成果

Adipose Tissue Remodeling under Ischemia: Death of Adipocytes and Activation of Stem/Progenitor Cells

• DOI: 10.1097/prs.0b013e3181f4468b
• 发表时间: 2010-12-01
• 期刊: PLASTIC AND RECONSTRUCTIVE SURGERY
• 影响因子: 3.6
• 作者: Suga, Hirotaka;Eto, Hitomi;Yoshimura, Kotaro
• 通讯作者: Yoshimura, Kotaro

Adipose injury-associated factors mitigate hypoxia in ischemic tissues through activation of adipose stem/stromal cells and induction angiogenesis

脂肪损伤相关因子通过激活脂肪干/基质细胞和诱导血管生成减轻缺血组织的缺氧

• 发表时间: 2012
• 期刊: American Journal of Pathology
• 影响因子: 6
• 作者: Eto H;et al.
• 通讯作者: et al.

Characterization of adipose tissue structure and cellular components : Differences between aspirated adipose tissue and excised adipose tissue

脂肪组织结构和细胞成分的表征：抽吸脂肪组织和切除脂肪组织之间的差异

• 发表时间: 2009
• 期刊: Plast Reconstr Surg
• 影响因子: 3.6
• 作者: Eto H;Suga H;Matsumoto D;Shigeura T;Inoue K;Aoi N;Kato H;Araki J;Yoshimura K
• 通讯作者: Yoshimura K

Functional Implications of CD34 Expression in Human Adipose-Derived Stem/Progenitor Cells

• DOI: 10.1089/scd.2009.0003
• 发表时间: 2009-10-01
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Suga, Hirotaka;Matsumoto, Daisuke;Yoshimura, Kotaro
• 通讯作者: Yoshimura, Kotaro