Development of angiogenic therapy with human Muse cells

人类 Muse 细胞血管生成疗法的发展

• 批准号: 25670749
• 负责人: YOSHIMURA Kotaro
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670749/
• 关键词: 創傷治癒; 幹細胞; 糖尿病; 皮膚潰瘍; 多能性幹細胞

SSEA-3+ multipotent mesenchymal cells (Muse cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Muse-rich ASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared to Muse-poor counterpart. Skin ulcers were generated in SCID mice with type 1 diabetes, which showed delayed wound healing compared to non-diabetic SCID mice. Treatment with Muse-rich cells significantly accelerated wound healing compared to treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated epidermis and dermis as epidermal keratinocytes and vascular endothelial cells, respectively. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound healing associated with type 1 diabetes.

从培养的人脂肪组织来源的干细胞/基质细胞(HASCs)中分离出SSEA-3+多能间充质细胞(MUSE细胞)，并对其进行鉴定，评价其治疗糖尿病皮肤溃疡的潜力。与缺乏MUSE的ASCs相比，MUSE丰富的ASCs分别表现出上调和下调的多能性和细胞增殖基因。患有1型糖尿病的SCID小鼠产生皮肤溃疡，与非糖尿病SCID小鼠相比，伤口愈合延迟。与缺乏缪斯细胞的治疗相比，富缪斯细胞的治疗显著加速了伤口的愈合。移植细胞分别作为表皮角质形成细胞和血管内皮细胞整合到再生的表皮和真皮中。然而，在周围完好无损的地区没有检测到它们。因此，选定的ASCs群体在加速与1型糖尿病相关的受损伤口愈合方面具有更大的治疗效果。