Development of angiogenic therapy with human Muse cells

人类 Muse 细胞血管生成疗法的发展

• 批准号: 25670749
• 负责人: YOSHIMURA Kotaro
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670749/
• 关键词: 創傷治癒; 幹細胞; 糖尿病; 皮膚潰瘍; 多能性幹細胞

SSEA-3+ multipotent mesenchymal cells (Muse cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Muse-rich ASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared to Muse-poor counterpart. Skin ulcers were generated in SCID mice with type 1 diabetes, which showed delayed wound healing compared to non-diabetic SCID mice. Treatment with Muse-rich cells significantly accelerated wound healing compared to treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated epidermis and dermis as epidermal keratinocytes and vascular endothelial cells, respectively. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound healing associated with type 1 diabetes.

从培养的人脂肪组织衍生的茎/基质细胞（HASC）中分离出SSEA-3+多能间充质细胞（MUSE细胞）并进行了表征，并评估了它们治疗糖尿病皮肤溃疡的治疗潜力。与Muse-Poor的对应物相比，Muse Frich ASC富裕的ASC分别表现出上调和下调的多能性和细胞增殖基因。与非糖尿病SCID小鼠相比，在患有1型糖尿病的SCID小鼠中产生了皮肤溃疡。与穆斯特贫民区的治疗相比，用富含缪斯博物馆的细胞治疗可显着加速伤口愈合。分别将移植的细胞整合到再生表皮和真皮中，分别为表皮角质形成细胞和血管内皮细胞。但是，在周围的完整区域未发现它们。因此，选定的ASC人群具有更大的治疗作用，以加速与1型糖尿病有关的伤口愈合受损。