Research on X-ray measurements of Remodeling Stress in Living Bone Tissue

活骨组织重塑应力的X射线测量研究

• 批准号: 12480254
• 负责人: TADANO Shigeru
• 金额: $ 6.46万
• 依托单位: HOKKAIDO UVIERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480254/
• 关键词: Biomechanics; Bone Structure; Remodeling; Cortical Bone; X-ray Diffraction; Residual Stress

Bone tissue is a composite material composed of hydroxyapatite (Hap) and collagen. Because Hap in bone tissue makes a crystal structure, an X-ray diffraction method is available to measure the strain of Hap. This study presents an X-ray diffraction method of measuring the residual stress/strain in bone tissue of rabbit's tibia . Bone powder of the diameter less than 40 micrometer was used as a control specimen at non-stressed state. From the measurements, it was clear that the distribution of residual stress existed in the bone tissue. The tensile residual stress at bone axial direction occurred in the proximal-medial region of rabbit's tibia. The compressive stress occurred in the other regions. In addition, the mechanism to generate the residual stress was investigated by sequential cutting of the tibia specimen from bone structure scale to bone tissue scale. The remodeling is a phenomenon that bone tissue and structure adapts functionally to mechanical environment. The residual stre … More ss will become a mechanical trigger to induce the remodeling.In the X-ray diffraction method using characteristic X-rays with a unique wavelength, Hap in compact bone has much lower crystallization than metal as steel. The peak position of diffracted intensity is not easy to determine form the gradual intensity-angle profile. This work proposes a method to calculate the lattice strain from the information of a whole diffraction profile without a peak position. In this experiment, strip specimens of 28x8x2 mm in size with their long axis aligned to the bone axial or the circumferential direction were cut from cortical bone in a shaft of bovine femur. To confirm the relationship between applied macroscopic strain and lattice strain in bone tissue, a four-point bending device was developed to apply bending load to the specimen during the X-ray irradiation. Macroscopic strain of the specimen was measured by a strain-gauge bonded to opposite side of the surface irradiated by X-rays. As a result, the accuracy of measurement by this method was better improved. Less

骨组织是由羟基磷灰石（Hap）和胶原蛋白组成的复合材料。由于骨组织中的 Hap 形成晶体结构，因此可以使用 X 射线衍射方法来测量 Hap 的应变。本研究提出了一种测量兔胫骨骨组织残余应力/应变的X射线衍射方法。以直径小于40微米的骨粉作为无应力状态下的对照标本。从测量结果可以清楚地看出骨组织中存在残余应力的分布。骨轴方向的残余拉应力发生在兔胫骨近内侧区域。压应力发生在其他区域。此外，通过连续切割胫骨标本，从骨结构尺度到骨组织尺度，研究了残余应力产生的机制。重塑是骨组织和结构在功能上适应力学环境的现象。残余应力将成为诱导重塑的机械触发器。在使用具有独特波长的特征X射线的X射线衍射方法中，致密骨中的Hap的结晶度比钢等金属低得多。从渐变的强度角度分布中不容易确定衍射强度的峰值位置。这项工作提出了一种根据没有峰值位置的整个衍射轮廓的信息来计算晶格应变的方法。在本实验中，从牛股骨干的皮质骨上切下尺寸为28x8x2mm的条状标本，其长轴与骨轴或圆周方向对齐。为了确认骨组织中施加的宏观应变和晶格应变之间的关系，开发了一种四点弯曲装置，在 X 射线照射期间向样本施加弯曲载荷。通过粘贴在受 X 射线照射的表面的另一侧的应变计来测量样品的宏观应变。因此，该方法的测量精度得到了较好的提高。较少的

项目成果

J.Shibano: "Residual Stress in Hydroxyapatite by X-ray Diffraction Method"Proceedings of Joint Symposium on Mechanics of New Materials. 13-14 (2003)

J.Shibano：“X射线衍射法研究羟基磷灰石中的残余应力”新材料力学联合研讨会论文集。

K.Fujisaki: "Lattice Strain Measurements of HAp Crystals in Bovine Cortical Bone"Proceedings of Joint Symposium on Mechanics of New Materials. 7-8 (2003)

K.Fujisaki：“牛皮质骨HAp晶体的晶格应变测量”新材料力学联合研讨会论文集。

S. Shibano, S. Tadano: "Residual Stress in HAP Coated Titanium Implant by X-ray Diffraction Method"Proceedings of International Congress on Biological and Medical Engineering. CD-ROM. (2002)

S. Shibano、S. Tadano：“通过 X 射线衍射法研究 HAP 涂层钛植入物中的残余应力”国际生物和医学工程大会论文集。

K.Fujisaki: "Lattice Strain of HAp Crystals in Bone Tissue Dsing X-ray Diffraction Method"Proceedings of International Congress on Biological and Medical Engineering, CD-ROM. (CD-ROM). (2002)

K.Fujisaki：“骨组织中 HAp 晶体的晶格应变 X 射线衍射法”国际生物和医学工程大会论文集，CD-ROM。

S.Tadano: "Residual Stress Measurements in Bone Tissue Using X-ray Diffraction"ASME International, Proc.ESDA2002. CD-ROM. BIO-001 (2002)

S.Tadano：“使用 X 射线衍射测量骨组织中的残余应力”ASME International，Proc.ESDA2002。