Development of analytical method for each domain of CTGF/ecogenin and its clinical application.

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Expression vectors for IGF binding protein-like(IGFBP), von Willebrand type C repeat(VWC), thrombospondin type I repeat(TSP1) and C-terminal(CT) domains of CTGF/ecogenin were constructed and their recombinant proteins were produced.Plasmids of CTGF lacking 1 or 2 domains were constructed and introduced into HeLa cells. Consequently, HeLa cell lines producing these proteins were established.Using the recombinant proteins, epitopes of six monoclonal antibodies and two polyclonal antibodies were determined. Three types of ELISA systems were developed.After production, CTGF was processed before it was secreted out of cells.CTGF was found to be a hypoxia-induced angiogenic factor, tumor angiogenesis factor and mechanical stress-induced factor. The ELISA systems described above could be used for diseases related with these phenomina.