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The role of hcs-24, a newly isolated hypertrophic chondrocyte-specific gene, in endochondral ossification

The role of hcs-24, a newly isolated hypertrophic chondrocyte-specific gene, in endochondral ossification
新分离的肥大软骨细胞特异性基因hcs-24在软骨内骨化中的作用
批准号:
08457490
负责人:
TAKIGAWA Masaharu
金额:
$1.22万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997

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中文摘要
翻译
1)克隆了肥大软骨细胞特异性基因hcs-24的c DNA序列。HCS-24编码区与结缔组织生长因子(CTGF)编码区核苷酸序列完全一致。2)在增生期培养的兔软骨细胞中,HCS-24/CTGF表达最高。用转化生长因子β和骨形态发生蛋白-2.3刺激软骨细胞的基因表达)免疫组织化学方法显示,小鼠肋骨软骨交界处肥大的软骨细胞和内皮细胞被抗CTGF抗体染色。4)在小鼠胚胎发育过程中,hcs-24/CTGF的表达水平在胚胎第7天达到最大值,在第17天逐渐下降,然后又升高。…基因对血管内皮细胞增殖和迁移能力的影响6)纯化的CTGF和重组CTGF均能刺激软骨细胞的增殖和蛋白多糖的合成,促进软骨细胞和成骨细胞的碱性磷酸酶的合成。生长因子模拟血管内皮细胞的增殖和迁移。7)建立了检测HCS-24/CTGF的酶联免疫吸附试验体系。8)在HCS-2/8细胞上发现了两种类型的特异性结合位点。(9)转OCNT/CTGF转基因小鼠骨骼结构紊乱,提示肥大软骨细胞合成的HCS-24/CTGF可刺激增殖软骨细胞的增殖成熟和成熟软骨细胞的肥大,诱导软骨细胞血管生成,促进软骨内成骨。该因子也可能参与胚胎的器官发生。较少
英文摘要
1) cDNA of hypertrophic chondrocyte specific gene hcs-24 was isolated. The nucleotide sequence of coding region of hcs-24 was completely the same as that of connective tissue growth factor (CTGF).2) Expression of hcs-24/ctgf in rabbit growth cartilage cells in culture was highest in hypertrophic stage. The gene expression in chondrocytic cells was stimulated by TGFbeta and BMP-2.3) Immunohistochemical techniques revealed that hypertrophic chondrocytes and endothelial cells in cost-chondral junctions of mouse ribs were stained with anti-CTGF antibody in vivo. Surface of and chondrocyte clusters in articular cartilge of arthritis were also stained with the antibody.4) During development of mouse embryos, mRNA level of hcs-24/ctgf reached a maximum at E7, decreased gradually and then increased again at E17.HCS-2/8 cells transfected with an hcs-24 expression vector grew rapidly than non-transfected cells. The abilities to proliferate and migrate of vascular endothelial cells transfected wi … More th expression vectors that generate anti-sense RNA of CTGF cDNA were markedly lower than those of control.6) Purified CTGF and recombinant CTGF stimulated the proliferation and proteoglycan synthesis of chondrocytes and alkaline phosphatase in chondrocytes and osteoblasts. The growth factor simulated the proliferation and migration of vascular endothelial cells. These effects were inhibited by anti-CTGF antibody.7) An ELISA system to measure Hcs-24/CTGF was established.8) Two types of specific binding sites of ^<125>I-rCTGF were identified on HCS-2/8 cells. The binding of ^<125>I-rCTGF to rabbit growth cartilage cells in culture was maximal in growth phase and decreased as they differentiated.9) Transgenic mice of OCNT/CTGF had skeletal disorder.These findings suggest that Hcs-24/CTGF synthesized by hpertrophic chondrocytes stimulates the proliferation and maturation of proliferative chondrocytes and hypertrophy of mature chondrocytes and induces angiogenesis into cartilage from bone, resulting in promotion of endochondral ossification. The factor may also be involved in organogenesis in embryos. Less
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Sakawa, A.: "Specific serum antibodies against membranous proteins of a human immortal chondrocytic cell line (HCS-2/8) in rheumatoid arthritis and their relation to the natural history of the disease" J.Bone Miner.Metab.14. 146-152 (1996)
Sakawa, A.:“类风湿关节炎中针对人类永生软骨细胞系 (HCS-2/8) 膜蛋白的特异性血清抗体及其与疾病自然史的关系”J.Bone Miner.Metab.14。
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滝川正春: "新たに樹立したヒト軟骨細胞様細胞株を用いた新規軟骨特異的遺伝子のクローニングと機能解析。" 山陽放送学術文化財団リポート. 40. 30-35 (1996)
Masaharu Takikawa:“使用新建立的人类软骨细胞样细胞系进行新型软骨特异性基因的克隆和功能分析”三洋广播科学基金会报告。 40. 30-35 (1996)
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21
    Neutrigenomics studies on endochondral ossification and articular cartilage mainteinance/regeneration
    • 批准号:
      17K19757
    • 项目类别:
      Grant-in-Aid for Challenging Research (Exploratory)
    • 资助金额:
      $4.16万
    • 财政年份:
      2017
    • 负责人:
      TAKIGAWA Masaharu
    • 依托单位:
    Establishment of molecular basis of CCN family proteins for therapeutic use and its related translational research
    • 批准号:
      15H05014
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.9万
    • 财政年份:
      2015
    • 负责人:
      TAKIGAWA Masaharu
    • 依托单位:
    A new protein transport system in cartilage: Multilayered transcytosis
    • 批准号:
      23659872
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.33万
    • 财政年份:
      2011
    • 负责人:
      TAKIGAWA Masaharu
    • 依托单位:
    Comprehensive study on molecular basis of actions of CCN family proteins as novel signal conductors and its translational application
    • 批准号:
      19109008
    • 项目类别:
      Grant-in-Aid for Scientific Research (S)
    • 资助金额:
      $70.72万
    • 财政年份:
      2007
    • 负责人:
      TAKIGAWA Masaharu
    • 依托单位:
    国内基金
    海外基金
    HIF-1α调控软骨细胞衰老在骨关节炎进展中的作用及机制研究
    • 批准号:
      82371603
    • 项目类别:
      面上项目
    • 资助金额:
      49.00万元
    • 批准年份:
      2023
    • 负责人:
      陈晓
    • 依托单位: