The role of hcs-24, a newly isolated hypertrophic chondrocyte-specific gene, in endochondral ossification
新分离的肥大软骨细胞特异性基因hcs-24在软骨内骨化中的作用
基本信息
- 批准号:08457490
- 负责人:
- 金额:$ 1.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) cDNA of hypertrophic chondrocyte specific gene hcs-24 was isolated. The nucleotide sequence of coding region of hcs-24 was completely the same as that of connective tissue growth factor (CTGF).2) Expression of hcs-24/ctgf in rabbit growth cartilage cells in culture was highest in hypertrophic stage. The gene expression in chondrocytic cells was stimulated by TGFbeta and BMP-2.3) Immunohistochemical techniques revealed that hypertrophic chondrocytes and endothelial cells in cost-chondral junctions of mouse ribs were stained with anti-CTGF antibody in vivo. Surface of and chondrocyte clusters in articular cartilge of arthritis were also stained with the antibody.4) During development of mouse embryos, mRNA level of hcs-24/ctgf reached a maximum at E7, decreased gradually and then increased again at E17.HCS-2/8 cells transfected with an hcs-24 expression vector grew rapidly than non-transfected cells. The abilities to proliferate and migrate of vascular endothelial cells transfected wi … More th expression vectors that generate anti-sense RNA of CTGF cDNA were markedly lower than those of control.6) Purified CTGF and recombinant CTGF stimulated the proliferation and proteoglycan synthesis of chondrocytes and alkaline phosphatase in chondrocytes and osteoblasts. The growth factor simulated the proliferation and migration of vascular endothelial cells. These effects were inhibited by anti-CTGF antibody.7) An ELISA system to measure Hcs-24/CTGF was established.8) Two types of specific binding sites of ^<125>I-rCTGF were identified on HCS-2/8 cells. The binding of ^<125>I-rCTGF to rabbit growth cartilage cells in culture was maximal in growth phase and decreased as they differentiated.9) Transgenic mice of OCNT/CTGF had skeletal disorder.These findings suggest that Hcs-24/CTGF synthesized by hpertrophic chondrocytes stimulates the proliferation and maturation of proliferative chondrocytes and hypertrophy of mature chondrocytes and induces angiogenesis into cartilage from bone, resulting in promotion of endochondral ossification. The factor may also be involved in organogenesis in embryos. Less
1)克隆了肥大软骨细胞特异性基因hcs-24的cDNA。hcs-24编码区核苷酸序列与结缔组织生长因子(CTGF)完全相同。2)hcs-24/CTGF在培养的兔生长软骨细胞中的表达在肥大期最高。TGF β和BMP-2刺激软骨细胞基因表达。3)免疫组化技术显示,抗CTGF抗体染色显示小鼠肋软骨连接处肥大的软骨细胞和内皮细胞。关节炎关节软骨表面和软骨细胞簇也被抗体染色。4)在小鼠胚胎发育过程中,hcs-24/ctgf的mRNA水平在E7时达到最大值,逐渐下降,然后在E17时再次升高。hcs-24表达载体转染的HCS-2/8细胞比未转染的细胞生长迅速。转染反义寡核苷酸的血管内皮细胞增殖和迁移能力的研究 ...更多信息 6)纯化的CTGF和重组CTGF能促进软骨细胞增殖、蛋白多糖合成及软骨细胞和成骨细胞碱性磷酸酶的合成。该生长因子可刺激血管内皮细胞的增殖和迁移。7)建立了Hcs-24/CTGF双抗体夹心ELISA检测方法; 8)<125>在HCS-2/8细胞上发现了两种不同的rCTGF特异性结合位点。9<125>)OCNT/CTGF转基因小鼠出现骨骼疾病,提示肥大软骨细胞合成的Hcs-24/CTGF刺激增殖软骨细胞的增殖和成熟,促进成熟软骨细胞的肥大,并诱导骨血管生成进入软骨,从而促进软骨内成骨。该因子也可能参与胚胎的器官发生。少
项目成果
期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shimo, T.: "Connective tissue growth factor is an autocrine factor stimulating the proliferation and migration of vascular endothelial cells." J.Biochem.(submitted). (1998)
Shimo, T.:“结缔组织生长因子是一种刺激血管内皮细胞增殖和迁移的自分泌因子。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakawa, A.: "Specific serum antibodies against membranous proteins of a human immortal chondrocytic cell line (HCS-2/8) in rheumatoid arthritis and their relation to the natural history of the disease" J.Bone Miner.Metab.14. 146-152 (1996)
Sakawa, A.:“类风湿关节炎中针对人类永生软骨细胞系 (HCS-2/8) 膜蛋白的特异性血清抗体及其与疾病自然史的关系”J.Bone Miner.Metab.14。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nakanishi, T.: Molecular Regulation of Endochondral Ossification. Molecular Bone Metabolism and Osteoporosis, (T.Matsumoto, ed.), 29-42 (1996)
Nakanishi, T.:软骨内骨化的分子调节。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
滝川正春: "新たに樹立したヒト軟骨細胞様細胞株を用いた新規軟骨特異的遺伝子のクローニングと機能解析。" 山陽放送学術文化財団リポート. 40. 30-35 (1996)
Masaharu Takikawa:“使用新建立的人类软骨细胞样细胞系进行新型软骨特异性基因的克隆和功能分析”三洋广播科学基金会报告。 40. 30-35 (1996)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tamura, T.: "Nitric oxide mediates interleukin-1-induced matrix degradation and basic fibroblast growth factor release in cultured rabbit articular chondrocytes : A possible mechanism of pathological neovascularization in arthritis" Endocrinology. 137. 37
Tamura, T.:“一氧化氮介导培养兔关节软骨细胞中白细胞介素 1 诱导的基质降解和碱性成纤维细胞生长因子释放:关节炎病理性新生血管形成的可能机制”内分泌学。
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TAKIGAWA Masaharu其他文献
TAKIGAWA Masaharu的其他文献
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{{ truncateString('TAKIGAWA Masaharu', 18)}}的其他基金
Neutrigenomics studies on endochondral ossification and articular cartilage mainteinance/regeneration
软骨内骨化和关节软骨维持/再生的中性基因组学研究
- 批准号:
17K19757 - 财政年份:2017
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Establishment of molecular basis of CCN family proteins for therapeutic use and its related translational research
CCN家族蛋白治疗用分子基础的建立及其相关转化研究
- 批准号:
15H05014 - 财政年份:2015
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A new protein transport system in cartilage: Multilayered transcytosis
软骨中新的蛋白质运输系统:多层转胞吞作用
- 批准号:
23659872 - 财政年份:2011
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Comprehensive study on molecular basis of actions of CCN family proteins as novel signal conductors and its translational application
CCN家族蛋白作为新型信号传导体的分子基础及其转化应用的综合研究
- 批准号:
19109008 - 财政年份:2007
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
The role of CTGF as a novel tissue-regenerating factor, regenerin, and its application for medical and dental tissue engineering
CTGF作为新型组织再生因子再生素的作用及其在医学和牙科组织工程中的应用
- 批准号:
15109010 - 财政年份:2003
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Functional analysis of the cartilage-derived multifunctional growth factor ecogenin/CTGF by using mutant animals
利用突变动物对软骨源性多功能生长因子 Ecogenin/CTGF 进行功能分析
- 批准号:
13307053 - 财政年份:2001
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Development of analytical method for each domain of CTGF/ecogenin and its clinical application.
CTGF/ecogenin各结构域分析方法的开发及其临床应用。
- 批准号:
12557154 - 财政年份:2000
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on the mechanism of actions of a cartilage-derived pleiotrophic growth factor, ecogenin/CTGF - Molecular cloning of its receptors and mechanism of inter- and intra signal transduction -
软骨源性多效生长因子 Ecogenin/CTGF 的作用机制研究 - 其受体的分子克隆以及内部和内部信号转导机制 -
- 批准号:
10470389 - 财政年份:1998
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Development of inhibitors for a angiogenesis factor CTGF and its application for angiogenetic diseases
血管生成因子CTGF抑制剂的研制及其在血管生成疾病中的应用
- 批准号:
10557165 - 财政年份:1998
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular cloning of novel osteoneurotrophins and their application as therapeutic agents for bone and cartilage diseases
新型骨神经营养蛋白的分子克隆及其在骨和软骨疾病治疗药物中的应用
- 批准号:
08557098 - 财政年份:1996
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
相似国自然基金
HIF-1α调控软骨细胞衰老在骨关节炎进展中的作用及机制研究
- 批准号:82371603
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