MOLECULAR ANALYSIS OF G6PD VARIANTS IN ASIA

亚洲 G6PD 变体的分子分析

• 批准号: 12576019
• 负责人: MATSUOKA Hiroyuki
• 金额: $ 4.29万
• 依托单位: JICHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576019/
• 关键词: GLUCOSE 6-PHOSPHATE DEHYDROGENASE; NUCLEOTIDE CHANGE; GENOMIC DNA; MALARIA; MOLECULAR EPIDEMIOLOGY; CHINA; NEPAL; ASIA; G6PD; PCR

In Nepal, we tested three hundred males for G6PD activity. Two persons were G6PD deficient (0.67 %). After getting an agreement of the investigation, we took 2ml of peripheral blood from them. We extracted genomic DNA from the blood and read sequence of DNA encoding G6PD molecule. Both persons were the same variant, G6PD Mediterranean, which had a replacement of C to T at No.563 of nuclcotide. Amino acid might change Scr to Phc at No.188 of amino-acid. These persons also had a replacement of C to T at No.1311 of nucleotide, which caused no replacement of amino acid. Last year, we found G6PD Mediterranean in a man of Malaysian Indian. His genomic DNA of G6PD molecule showed a replacement of C to T at No.563 nucleotide. However nucleotide No.1311 was C, which was as same as the wild type. This G6PD Mediterranean in Malaysia was original Indian type. G6PD Mediterranean found in Nepal was the type which was reported in Middle East countries and Mediterranean countries. These results indicate that the origin of Nepalese people is closer to people in Middle East countries than people in India. (190 words)

在尼泊尔，我们对300名男性进行了G6 PD活性检测。G6PD缺乏2例（0.67%）。在征得调查同意后，我们从他们身上采集了2ml外周血。我们从血液中提取基因组DNA并读取编码G6PD分子的DNA序列。两人均为同一变异型，G6PD地中海型，其563号核苷酸处C替换为T。氨基酸可能使Scr在第188位变为Phc。这些人也有一个C到T在第1311位的核苷酸的替代，这不会导致氨基酸的替代。去年，我们在一个马来西亚印度人身上发现了G6PD地中海。其G6PD分子基因组DNA第563位核苷酸由C变为T。而第1311位核苷酸为C，与野生型相同。马来西亚的G6PD地中海是原始的印度类型。在尼泊尔发现的G6PD地中海型是中东国家和地中海国家报告的类型。这些结果表明，尼泊尔人的起源更接近于中东国家的人，而不是印度人。(190字）

项目成果

Da Silveira LA., Matsuoki H. et al.: "Sequence diversity and linkage disequilibrium within the merozoite surface protein-1 (Msp-1) locus of Plasmodium falciparum: a longitudinal study in Brazil."J Eukaryot Microbiol. 48 (4). 433-9 (2001)

Da Silveira LA.、Matsuoki H. 等人：“恶性疟原虫裂殖子表面蛋白 1 (Msp-1) 位点内的序列多样性和连锁不平衡：巴西的一项纵向研究。”J Eukaryot Microbiol。

Da Silveira LA, Matsuoka H, ほか: "Sequence diversity and linkage disequilibrium within the merozoite surface protein-1(Map-1)locus of Plasmodium falciparum a longitudinal study in Brazil"J Eukaryot Microbiol. 48(4). 433-439 (2001)

Da Silveira LA、Matsuoka H 等人：“巴西恶性疟原虫裂殖子表面蛋白-1（Map-1）基因座内的序列多样性和连锁不平衡”J Eukaryot Microbiol 48（4）。 433-439（2001）

Win T. T., Lin L., Matsuoka H. et al.: "Detection of Plasmodium ovale by the ICT malaria P.f./P.v. immunochromatographic test"Acta Tropica. 80 (3). 283-284 (2001)

Win T. T.、Lin L.、Matsuoka H. 等人：“通过 ICT 疟疾 P.f./P.v. 免疫色谱测试检测卵形疟原虫”Acta Tropica。

Iwai K.,Hirono A.,Matsuoka H. ほか: "Distribution of glucose-6-phosphate dehydrogenase mutations in Southeast Asia."Human Genetics. (in press).

Iwai K.、Hirono A.、Matsuoka H. 等人：“东南亚葡萄糖-6-磷酸脱氢酶突变的分布”。人类遗传学（出版中）。

Iwai K, Hirono A, Matsuoka Hほか: "Distribution of glucose-6-phosphate dehydrogenase mutations in Southeast Asia"Human Genetics. 108(6). 445-449 (2001)

Iwai K，Hirono A，Matsuoka H，等人：“东南亚葡萄糖-6-磷酸脱氢酶突变的分布”人类遗传学108（6）（2001）。