Molecular analyses of G6PD variants in malaria endemic areas in Asian and African countries

点击翻译按钮获取中文摘要

We visited malaria endemic areas in Asian and African countries to introduce rapid methods for malaria diagnosis and glucose-6-phosphate dehydrogenase (G6PD) activity test. For malaria diagnosis, acridine orange method was employed. For G6PD activity test, WST-8 method was used. These methods were done at the site of malaria endemic areas and results were informed within 30 minutes after taking blood. Patients could receive anti-malarial medicine accordingly including primaquine to eliminate gametocytes. Primaquine was not used for the patients with G6PD deficiency. WST-8 method was useful for the detection of G6PD deficiency in rural areas without electricity and was recommendable for use in malaria control programs. We found more than 700 G6PD deficient people in these activities. DNA analysis of G6PD gene was performed after receiving informed consent. Thus we read about 400 samples and found 22 molecular variants including 5 new G6PD variants.