STUDIES ON GAMETOCYTA ACTIVATING FACTOR IN THE SALIVARY GRAND OF ANOPHELINE MOSQUITOES

按蚊唾液腺配子体激活因子的研究

• 批准号: 12670238
• 负责人: MATSUOKA Hiroyuki
• 金额: $ 2.18万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670238/
• 关键词: MALARIA; GAMETOCYTE; EXFLAGELLATION; SALIVARY GLAND; ANOPHELES; AEDES; CULEX; MOSQUITO; キサントウレイン酸

Gametocyte activating factor (GAF) induces exflagellation of Plasmodium microgametes. We found GAF in the salivary glands of female mosquitoes, Anopheles stephensi. The exflagellation was induced in a concentration-dependent manner in the supernatant of salivary gland's crude homogenate. The exflagellation inducing activity (EIA) in the salivary gland was higher than that in the midgut and the head. GAF in the salivary glands was found to be heat stable and low molecule (< 3,000 molecular weight). Analysis of the supernatant by capillary electrophoresis and UV absorbance profile showed that the salivary glands contained xanthurenic acid, which was previously identified as GAF in the head of A. stephensi. EIA in the salivary gland declined immediately after a blood meal, implying that GAF was in the saliva, and was delivered into the midgut together with the blood and induced exflagellation in the midgut. Next, we compared EIA in the salivary glands of five mosquito species, Aedes aegypti, Ae. Albopictus, An. omorii, An. Stephensi and Culex pipiens pallens. As a result, all the mosquito species examined contained GAF in the salivary glands. An. omorii and An. Stephensi consumed more GAF than Ae. aegypti, Ae. albopictus and Cx. p. pallens during blood feeding, suggesting that more GAF might be introduced into the midgut of Anopheles mosquitoes. This would be one of the reasons why only anopheline mosquitoes can transmit mammalian malaria. (224 words)

配子细胞活化因子（GAF）诱导疟原虫小配子脱落。我们在雌蚊斯氏按蚊的唾液腺中发现了GAF。在唾液腺粗匀浆上清液中以浓度依赖的方式诱导鞭毛。唾液腺的鞭毛诱导活性（EIA）高于中肠和头部。唾液腺中的GAF是热稳定的低分子（< 3,000分子量）。毛细管电泳和紫外吸光度分析表明，唾液腺中含有黄嘌呤酸，该物质先前被鉴定为斯蒂芬氏盲蝽头部的GAF。血餐后唾液腺的EIA立即下降，表明GAF存在于唾液中，随血液一起进入中肠，引起中肠的鞭毛。接下来，我们比较了埃及伊蚊、伊蚊、伊蚊等5种蚊子唾液腺的EIA。排,一个。omorii,。斯氏蚊和淡色库蚊。结果，所有被检测的蚊子在唾液腺中都含有GAF。一个。小森和安。史蒂芬氏蝇消耗的GAF多于白蛉。蚊,Ae。白纹伊蚊和Cx。提示可能有更多GAF被引入按蚊的中肠。这可能是为什么只有按蚊可以传播哺乳动物疟疾的原因之一。(224字)

项目成果

Luo,E.,Matsuoka,H. ほか: "Changes of salivary proteins during feeding and detection of saliva proteins in the midgut after feeding in a malaria vector mosquito, Anopheles stephensi (Diptera : Culicidae)."Medical Entomology and Zoology. 51. 13-20 (2000)

Luo, E., Matsuoka, H. 等人：“疟疾媒介蚊子按蚊（双翅目：蚊科）进食期间唾液蛋白的变化以及中肠唾液蛋白的检测。”医学昆虫学和动物学。 51. 13-20 (2000)

Matsuoka H., Yoshida S., Hirai M., Ishii A.: "A rodent malaria, Plasmodium berghei, is experimentally transmitted to mice by merely probing of infective mosquito Anopheles stephensi"Parasitol Int. 51(1) : (in press). (2002)

Matsuoka H.、Yoshida S.、Hirai M.、Ishii A.：“一种啮齿类疟疾，伯氏疟原虫，仅通过探测传染性蚊子斯氏按蚊，就在实验上传播给小鼠”Parasitol Int。

Uchida K., Oda T., Matsuoka H., Moribayashi A., Ohmori D., Eshita Y., Fukunaga A.: "Induction of oogenesis in mosquitoes (Diptera : Culicidae) by infusion of the hemocoel with amino acids"J Med Entomol. 38 (4). 572-575 (2001)

Uchida K.、Oda T.、Matsuoka H.、Moribayashi A.、Ohmori D.、Eshita Y.、Fukunaga A.：“通过向血腔注入氨基酸诱导蚊子（双翅目：蚊科）卵子发生”J Med

Matsuoka H, Yoshida S, Hirai M, Ishii A: "A rodent malaria, Plasmodium berghei, is experimentally transmitted to mice by merely probing of infective mosquito Anopheles stephensi"Parasitology International. 51(1)(In press). (2002)

Matsuoka H、Yoshida S、Hirai M、Ishii A：“一种啮齿动物疟疾，伯氏疟原虫，仅通过探测传染性蚊子斯氏按蚊就在实验上传播给小鼠”国际寄生虫学。

Hirai M., Wang J., Yoshida S., Ishii A., Matsuoka H.: "Characterization and identification of exflagellation inducing factor in the salivary gland of Anopheles stephensi (Diptera: Culicidae)"Biochem Biophys Res Commun. 287 (4). 859-864 (2001)

Hirai M.、Wang J.、Yoshida S.、Ishii A.、Matsuoka H.：“史氏按蚊（双翅目：蚊科）唾液腺中鞭毛诱导因子的表征和鉴定”Biochem Biophys Res Commun。