Molecular analysis of G6PD variants in Asian Countries

我们在亚洲国家的偏远地区进行了疟疾诊断和葡萄糖-6-磷酸脱氢酶（G6PD）检测的调查。我们用手指刺血法从候选人身上取了两滴血。一滴血用于吖啶橙法诊断疟疾，另一滴血用于WST-8检测G6PD。两项诊断均在30分钟内完成。当患者存在疟原虫且G6PD正常时，可给予包括伯氨喹在内的抗疟药物。我们采集了少量G6PD缺陷患者的血样和知情同意书，分析了G6PD基因的DNA序列。在缅甸，超过90%的G6PD缺乏者表现为G6PD Mahidol型（487G>A）。然而在柬埔寨，超过90%的G6PD缺乏者表现为G6PD Viangchan型（871G>A）。这些结果表明，缅甸人和柬埔寨人都是独立的同源祖先，在G6PD变异方面存在差异

We conducted surveies of malaria diagnoses and glucose-6-phosphate dehydrogenase (G6PD) testing in remote areas of Asian countries. We took two drops of blood from the candidates by the finger prick method. One drop of blood was used for malaria diagnosis of Acridine orange method, and another drop of blood was for G6PD test using WST-8. Both diagnoses were completed within 30 minutes. Patients could receive anti-malarial medicine including primaquine when they had malaria parasites and their (G6PD conditions were normal. We obtained a small amount of blood from G6PD deficient subjects as well as informed consents, and analyzed DNA sequenses of G6PD gene. In Myanmar, more than 90% of G6PD deficient subjects showed G6PD Mahidol type (487G>A). However in Cambodia, more than 90% of G6PD deficient subjects showed G6PD Viangchan type (871G>A). These results indicate that both Myanmar people and Cambodian people are independently derived from homogeneous ancestries and different each other in terms of G6PD variant