Molecular analysis of G6PD variants in Asian Countries

亚洲国家 G6PD 变异的分子分析

• 批准号: 14406026
• 负责人: MATSUOKA Hiroyuki
• 金额: $ 6.02万
• 依托单位: JICHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14406026/
• 关键词: G6PD; malaria; molecular epidemiology; human genetics; metabolic abnormality; primaquine; anemia; Asia; ベトナム; カンボジア; 韓国; ネパール; インドネシア; ミャンマー; 中国

We conducted surveies of malaria diagnoses and glucose-6-phosphate dehydrogenase (G6PD) testing in remote areas of Asian countries. We took two drops of blood from the candidates by the finger prick method. One drop of blood was used for malaria diagnosis of Acridine orange method, and another drop of blood was for G6PD test using WST-8. Both diagnoses were completed within 30 minutes. Patients could receive anti-malarial medicine including primaquine when they had malaria parasites and their (G6PD conditions were normal. We obtained a small amount of blood from G6PD deficient subjects as well as informed consents, and analyzed DNA sequenses of G6PD gene. In Myanmar, more than 90% of G6PD deficient subjects showed G6PD Mahidol type (487G>A). However in Cambodia, more than 90% of G6PD deficient subjects showed G6PD Viangchan type (871G>A). These results indicate that both Myanmar people and Cambodian people are independently derived from homogeneous ancestries and different each other in terms of G6PD variant

我们在亚洲国家的偏远地区进行了疟疾诊断和葡萄糖-6-磷酸脱氢酶（G6 PD）检测的调查。我们用手指针刺法从候选人身上取了两滴血。一滴血用于吖啶橙子法疟疾诊断，另一滴血用于WST-8法G6 PD试验。两次诊断均在30分钟内完成。当患者有疟疾寄生虫并且其G6 PD状况正常时，可以接受包括伯氨喹在内的抗疟疾药物。采集G6 PD缺陷者及知情同意者的少量血样，进行G6 PD基因的DNA序列分析。在缅甸，超过90%的G6 PD缺乏受试者表现为G6 PD Mahidol型（487 G>A）。然而，在柬埔寨，超过90%的G6 PD缺乏受试者表现为G6 PD维昂占型（871 G>A）。这些结果表明，缅甸人和柬埔寨人都是独立的同源祖先，在G6 PD变异方面彼此不同

项目成果

Matsuoka H et al.: "Two cases of glucose-6-phophate dehydrogenase deficient Nepalese belonging to the G6PD Mediterranean-type, not India-Pakistan sub-type but Mediterranean-Middle East sub-type"Journal of Human Genetics. 48・5. 275-277 (2003)

Matsuoka H等人：“两例葡萄糖-6-磷酸脱氢酶缺陷型尼泊尔人属于G6PD地中海型，不是印度-巴基斯坦亚型，而是地中海-中东亚型”《人类遗传学杂志》48・5。 .275-277 (2003)

G6PD mutations in Myanmar. G6PD Mahidol(487G>A) is the most common variant in the Myanmar population

缅甸的 G6PD 突变。

• 发表时间: 2004
• 期刊: Journal of Human Genetics 49・10
• 作者: Matsuoka H. et al.

An improved, simple screening method for detection of G6PD deficiency.

一种改进的、简单的 G6PD 缺陷检测筛查方法。

• 发表时间: 2003
• 期刊: Tropical Medicine and International Health 8
• 作者: Tantular IS et al.

Glucose-6-phosphate dehydrogenase (G6PD) mutations in Myanmar : G6PD Mahidol (487G>A) is the most common variant in the Myanmar population.

缅甸的 6-磷酸葡萄糖脱氢酶 (G6PD) 突变：G6PD Mahidol (487G>A) 是缅甸人群中最常见的突变。

• 发表时间: 2004
• 期刊: J Hum Genet 49(10)
• 作者: Matsuoka H;Wang J;Hirai M;Arai M;Yoshida S;Kobayashi T;Jalloh A;Lin K;Kawamoto F.
• 通讯作者: Kawamoto F.

Jalloh A et al.: "Rapid epidemiologic assessment of glucose-6-phosphate dehydrogenase (G6PD) deficiency in malaria-endemic areas in Southeast Asia using a novel diagnostic kit"Tropical Medicine and International Health. 9・5(印刷中). (2004)

Jalloh A 等人：“使用新型诊断试剂盒对东南亚疟疾流行地区葡萄糖-6-磷酸脱氢酶 (G6PD) 缺乏症进行快速流行病学评估”《热带医学与国际健康》，2004 年。 ）