How malaria parasites move from the skin to the liver or other organs after injected by mosquitoes?

疟原虫被蚊子注射后如何从皮肤转移到肝脏或其他器官？

• 批准号: 16590344
• 负责人: MATSUOKA Hiroyuki
• 金额: $ 2.3万
• 依托单位: JICHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590344/
• 关键词: malaria; transgenic parasite; life cycle; luciferase; luciverin; anopheline mosquito; green fluorescent protein; ルシフェラーゼ; スポロゾイト; 唾液腺

To observe how malaria parasites develop in living mosquitoes or mice, we prepared transgenic malaria parasites (Plasmodium berghei). Green fluorescent protein (GFP) gene or luciferase gene were introduced with DHFR-TS (pyrimethamine resistant) gene. Because the GFP transgenic parasites gave fluorescent when they received UV light, we could observe malaria parasites in mosquito midgut and salivary gland. When we used luciferase transgenic parasites, luciferase activity in mice was visualized through the imaging of whole bodies or dissected organs by using an in-vivo Imaging System (IVIS). Oocysts on the midgut and sporozoites in the salivary glands in mosquitoes were visualized with GFP. When we adopted luciferase-transgenic parasites, we obtained a phenomenon that sporozoites stay alive in the skin more than 24 hours after probing by infective mosquitoes. Forty-two hours after the probing, luciferase-transgenic parasites. were developed in the liver. We confirmed in the present study that liver is the specific organ for the malaria parasites for developing in number before invading in the erythrocytes. Furthermore we observed some lymph nodes giving luciferase activity at 42 hours after probing, suggesting that malaria parasites develop in the lymph nodes.

为了观察疟原虫如何在活蚊子或小鼠中发育，我们制备了转基因疟原虫（伯氏疟原虫）。将绿色荧光蛋白（GFP）基因或荧光素酶基因与DHFR-TS（抗乙胺嘧啶）基因一起导入。由于GFP转基因疟原虫在紫外光照射下发出荧光，我们可以在蚊子的中肠和唾液腺中观察到疟原虫。当我们使用荧光素酶转基因寄生虫时，通过使用体内成像系统（IVIS）对整个身体或解剖器官进行成像，来可视化小鼠中的荧光素酶活性。用GFP观察蚊子中肠上的卵囊和唾液腺中的子孢子。当我们采用转基因寄生虫时，我们获得了子孢子在感染性蚊子探测后在皮肤中存活超过24小时的现象。探测42小时后，转基因寄生虫。都是在肝脏中形成的我们在本研究中证实，肝脏是疟原虫在侵入红细胞之前进行数量发育的特定器官。此外，我们观察到一些淋巴结在探测后42小时给出荧光素酶活性，这表明疟疾寄生虫在淋巴结中发育。

项目成果

The effects of blood feeding and exogenous supply of tryptophan on the quantities of xanthurenic acid in the salivary glands of Anopheles stephensi (Diptera : Culicidae)

血液喂养和外源色氨酸供应对史氏按蚊（双翅目：蚊科）唾液腺黄嘌呤酸含量的影响

• 发表时间: 2006
• 期刊: Biochem Biophys Res Commun 341(4)
• 作者: Okech BA;Arai M;Matsuoka H
• 通讯作者: Matsuoka H

Rapid epidemiologic assessment of glucose-6-phosphate dehydrogenase deficiency in malaria-endemic areas in Southeast Asia using a novel diagnostic kit

• DOI: 10.1111/j.1365-3156.2004.01237.x
• 发表时间: 2004-05-01
• 期刊: TROPICAL MEDICINE & INTERNATIONAL HEALTH
• 影响因子: 3.3
• 作者: Jalloh, A;Tantular, IS;Kawamoto, F
• 通讯作者: Kawamoto, F

The effects of blood feeding and exogenous supply of tryptophan on the quantities of xanthurenic acid in the salivary glands of Anopheles stephensi(Diptera : Culicidae)

血液喂养和外源色氨酸供应对史氏按蚊（双翅目：蚊科）唾液腺黄嘌呤酸含量的影响

• 发表时间: 2006
• 期刊: Biochem Biophys Res Commun 341(4)
• 作者: Okech BA;Arai M;Matsuoka H
• 通讯作者: Matsuoka H

G6PD mutations in Myanrnar. G6PD Mahidol (487G>A) is the most common variant in the Myanmar population

缅甸的 G6PD 突变。

• 发表时间: 2004
• 期刊: Journal of Human Genetics 49・10
• 作者: Nakanishi H;Ito S;Mochizuki Y;Tatematsu M.;Matsuoka H. et al.
• 通讯作者: Matsuoka H. et al.

Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia : G6PD Viangchan (871G > A) is the most common variant in the Cambodian population.

柬埔寨的 6-磷酸葡萄糖脱氢酶 (G6PD) 突变：G6PD Viangchan (871G > A) 是柬埔寨人群中最常见的突变。

• 发表时间: 2005
• 期刊: J. Human Genet. 50
• 作者: Matsuoka;H. et al.
• 通讯作者: H. et al.