ultra-high performance liquid chromatography–triple quadrupole mass spectrometer

超高效液相色谱三重四极杆质谱仪

• 批准号: 461087410
• 金额: --
• 依托单位: Universität Hohenheim
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/461087410?language=en
• 关键词: ultra; performance; liquid; chromatography; triple

The requested mass spectrometer will particularly be used for the quantitation of bioactive food ingredients, whose presence can either be desired (e.g., aroma- and taste-active compounds) or undesired (e.g., processing contaminants with toxicological relevance – „food-borne toxicants“). Besides, the analysis of toxicologically relevant residues and contaminants (e.g., pesticides, tropane and pyrrolizidine alkaloids, mycotoxins) will be performed with the new instrument (Department of Food Chemistry and Analytical Chemistry 170a, Institute of Food Chemistry). Mainly triple quadrupole (QqQ) or QTRAP mass spectrometer are suitable for quantitation experiments due to the possibility to analyze the analytes and the corresponding stable isotopically labeled internal standards very selectively and sensitively via selected reaction monitoring (SRM) or multiple reaction monitoring (MRM). In comparison to other detector types, pairs of precursor and product ions can be analyzed in a single SRM experiment, which is specific for the respective analyte and standard, respectively. The instrument in the chosen specification enables application in ultra-trace amounts, which is of high importance for toxicologically relevant compounds in foods (especially in food for infants and children). Additionally, the applied instrument offers the ion mobility option, enabling the analysis of isobaric molecules.The intended working hours for the topic lipids and polyhalogenated compounds as well as the analysis of vitamins including studies on bioavailability and metabolism performed by the co-applicants Prof. Vetter (Department of Food Chemistry 170b, Institute of Food Chemistry) and Prof. Frank (Department of Food Biofunctionality 140b, Institute of Nutritional Sciences) ideally complement the use of the instrument.Finally, the requested instrument is a high-class and very flexible system enabling scientific research and subsequently answers on a broad analytical basis both for the research interests of the main applicant and both co-applicants at the University of Hohenheim and will have a huge impact on the continuous extension of national and international scientific success.

所要求的质谱仪将特别用于生物活性食品成分的定量，其存在可能是所希望的(例如，香气和味道活性化合物)，也可能是不希望的(例如，处理与毒理学相关的污染物--“食源性有毒物质”)。此外，与毒性有关的残留物和污染物(如杀虫剂、托烷和吡咯里西丁生物碱、真菌毒素)的分析将使用新仪器(食品化学和分析化学系170a，食品化学研究所)进行。主要是三重四极杆(QQQ)或QTRAP质谱仪适合于定量实验，因为可以通过选择反应监测(SRM)或多反应监测(MRM)非常选择性和灵敏地分析分析物和相应的稳定同位素标记内标。与其他类型的检测器相比，可以在单个SRM实验中分析成对的前体离子和产物离子，这分别针对各自的分析物和标准。所选规格中的仪器可用于超痕量，这对食品(特别是婴儿和儿童食品)中的有毒相关化合物具有非常重要的意义。此外，应用的仪器提供了离子迁移率选项，使得能够分析等压分子。主题脂质和多卤代化合物的预定工作时间以及维生素的分析包括由共同申请人Vetter教授(食品化学研究所食品化学系170b)和Frank教授(营养科学研究所食品生物功能性系140b)执行的关于生物利用度和新陈代谢的研究理想地补充了该仪器的使用。最后，所要求的仪器是一种高级和非常灵活的系统，能够进行科学研究，随后在广泛的分析基础上回答霍亨海姆大学主要申请者和共同申请者的研究兴趣，并将对不断扩大国家和国际科学成就产生巨大影响。