Clinical application and functional analysis of MAGE E1 gene, glioma specific new gene belonging MAGE family

MAGE- e1是黑色素瘤相关抗原基因（MAGE）家族的一员，在恶性胶质瘤中相对于正常中枢神经系统细胞表达上调。在本文中，我们鉴定了MAGE-E1蛋白并研究了其在胶质瘤细胞中的表达。用与MAGE-E1蛋白片段匹配的14聚肽免疫的三只兔子产生抗体。Western blot和免疫细胞化学分析显示该蛋白定位于细胞质中。在部分U87MG细胞中，该蛋白在细胞核周围富集。在10%胎牛血清培养的T98G细胞中，MAGE-E1蛋白表达较高，而在血清饥饿72小时的生长阻滞细胞中，MAGE-E1蛋白表达较低。20% FBS刺激生长停滞细胞后，表达上调。对患者切除的胶质瘤进行Western blot分析和免疫组化，结果显示，在胶质母细胞瘤中，MAGE-E1蛋白的表达水平高于弥漫性星形细胞瘤。我们的研究结果表明MAGE-E1在胶质瘤细胞的增殖中起作用。MAGE-E1可能是治疗恶性胶质瘤的靶分子。

MAGE-E1 is a member of the melanoma-associated antigen gene (MAGE) family that is up-regulated in malignant glioma relative to normal central nervous system cells. In this manuscript, we identified MAGE-E1 protein and studied its expression in glioma cells. Antibodies were produced in three rabbits immunized with a 14-mer peptide that matched a MAGE-E1 protein segment. Western blot analysis and immunocytochemistry of U87MG cells showed the protein to be localized in the cytoplasm. The protein was enriched around the nucleus in some of U87MG cells. MAGE-E1 protein expression was high in T98G cells cultured with 10% fetal bovine serum but low in growth-arrested cells that were serum-starved for 72 hours. The expression was up-regulated by stimulation of growth-arrested cells with 20% FBS. Western blot analysis and immunohistochemistry of gliomas resected from patients showed MAGE-E1 protein to be present at higher levels in glioblastomas than in diffuse astrocytomas. Our results suggest that MAGE-E1 play a role in the proliferation of glioma cells. MAGE-E1 might be a target molecule for treatment of malignant gliomas.