Analysis of the changes in brain organic osmolytes in cerebral ischemia

脑缺血时脑有机渗透压物质的变化分析

• 批准号: 08671582
• 负责人: YOSHIMINE Toshiki
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671582/
• 关键词: cerebral ischemia; brain edema osmolyte; amino acid; methylamines; myo-inositol; magnetic resonance spectroscopy

The cell volume is regulated not only by inorganic ions, but also by organic osmolytes, such as amino acids, methylamines, and polyhydric alcohols (polyols). Using proton nuclear magnetic resonance spectroscopy (^1H-NMR), we measured the tissue concentrations of those organic osmolytes in the rat brain after middle cerebral artery occlusion (incomplete focal ischemia) or after decapitation (complete global ischemia). The total osmolytes expressed as a sum of total amino acids, total methylamines, and total polyols were significantly decreased at 24 hours of focal ischemia (58.7% of control value, P=0.0025) whereas they were not changed following decapitation. The water content was increased from control value of 77.9% to 84.1% after focal ischemia (P<0.0001) bit not after decapitation. These results suggested that the brain organic osmolytes are involved in the process of edema formation following focal cerebral ischemia. Further elucidation of the cellular mechanisms regulating these organic osmolytes in cerebral ischemia may promote greater understanding of pathophysiology of brain edema as well as the development of novel measures of treatment.

细胞体积不仅由无机离子调节，还由有机渗透剂调节，如氨基酸、甲胺和多元醇（多元醇）。利用质子核磁共振波谱（^1H-NMR），我们测量了大脑中动脉闭塞（不完全局灶性缺血）或断头（完全全脑缺血）后大鼠脑中这些有机渗透剂的组织浓度。以总氨基酸、总甲胺和总多元醇的总和表示的总渗透压物质在局灶性缺血24小时时显著降低（为对照值的58.7%，P=0.0025），而在断头后它们没有变化。脑含水量由对照组的77.9%增加到84.1%（P<0.0001），而断头后无明显变化。提示脑有机渗透物质参与了局灶性脑缺血后水肿的形成过程。进一步阐明这些有机渗透调节剂在脑缺血的细胞机制，可能会促进更好地了解脑水肿的病理生理学，以及新的治疗措施的发展。

项目成果

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