Study for regulation of gonadotropin receptor and development of new ovulation induction method

我们观察了转化生长因子-β对大鼠颗粒细胞卵泡刺激素和黄体生成素受体表达的影响。与对照组相比，转化生长因子-β以剂量依赖的方式增加FSH-RmRNA水平，最大约为对照组的2倍。此外，促卵泡激素可显著增加促黄体生成素受体基因的表达，且随着β浓度的增加，促卵泡激素诱导的促黄体生成素受体基因表达呈剂量依赖性增加。促性腺激素显著增强卵泡刺激素和促黄体生成素受体5‘侧翼区的活性，但转化生长因子-β对促卵泡刺激素诱导的促性腺激素活性无明显影响。另一方面，加入转化生长因子-β后，颗粒细胞中FSH-Rm RNA和Lh-R m RNA的半衰期显著延长。转化生长因子-β还以剂量和时间依赖的方式刺激卵泡抑素m RNA的表达积累。卵泡刺激素不影响转化生长因子-β诱导的卵泡刺激素受体基因表达。因此，转化生长因子-β对卵泡刺激素受体和促黄体生成素受体的影响可能是由于受体的稳定性增加和卵泡抑素的不敏感所致。

We investigated the effect of TGF-β on the expression of FSH and LH receptor expression in the rat granulasa cells. TGF-β increased FSH-R mRNA levels with a maximum of about a 2-fold in a dose-dependent manner compared to the control. In addition, treatment with FSH produced a substantial increase in LH-R mRNA, and concurrent treatment with increasing concentration of TGF-β brought about dose-dependent increases in FSH-induced LH-R mRNA. FSH significantly enhanced the activity of FSH and LH receptor 5'-flanking region, but treatment with TGF- β did not significantly influence the activity induced by FSH. On the other hand, the decay curves for FSH-R mRNA and LH-R mRNA transcripts in granulosa cells showed a significant increase in half-life after the addition of TGF-β.TGF-β also stimulated the expression of follistatin mRNA accumulation in a dose- and time- dependent manner. Follistatin did not affect TGF-β-induced FSH-R mRNA. Therefore, the profile of the TGF-β effect on FSH-R mRNA and LH-R mRNA may be due to the increased stability of both receptor mRNAs and insensitivity of the follistatin.