Study for regulation of gonadotropin receptor and development of new ovulation induction method

促性腺激素受体调控研究及促排卵新方法开发

• 批准号: 13470344
• 负责人: MINEGISHI Takashi
• 金额: $ 8万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470344/
• 关键词: FSH receptor; LH receptor; TGF-β; Activin; Follistatin; StAR; Granulosa cell; フォリスタチン; ステロイド産生

We investigated the effect of TGF-β on the expression of FSH and LH receptor expression in the rat granulasa cells. TGF-β increased FSH-R mRNA levels with a maximum of about a 2-fold in a dose-dependent manner compared to the control. In addition, treatment with FSH produced a substantial increase in LH-R mRNA, and concurrent treatment with increasing concentration of TGF-β brought about dose-dependent increases in FSH-induced LH-R mRNA. FSH significantly enhanced the activity of FSH and LH receptor 5'-flanking region, but treatment with TGF- β did not significantly influence the activity induced by FSH. On the other hand, the decay curves for FSH-R mRNA and LH-R mRNA transcripts in granulosa cells showed a significant increase in half-life after the addition of TGF-β.TGF-β also stimulated the expression of follistatin mRNA accumulation in a dose- and time- dependent manner. Follistatin did not affect TGF-β-induced FSH-R mRNA. Therefore, the profile of the TGF-β effect on FSH-R mRNA and LH-R mRNA may be due to the increased stability of both receptor mRNAs and insensitivity of the follistatin.

本实验观察了TGF-β对大鼠颗粒细胞FSH和LH受体表达的影响。与对照组相比，TGF-β以剂量依赖性方式增加FSH-R mRNA水平，最大约为2倍。此外，FSH处理导致LH-R mRNA显著增加，同时增加TGF-β浓度导致FSH诱导的LH-R mRNA剂量依赖性增加。FSH能显著增强FSH和LH受体5 '侧翼区的活性，而TGF- β对FSH诱导的活性无显著影响。另一方面，颗粒细胞中FSH-R mRNA和LH-R mRNA转录本的衰减曲线显示，加入TGF-β后，半衰期显著增加，TGF-β还以剂量和时间依赖的方式刺激卵泡抑素mRNA的表达。卵泡抑素对TGF-β诱导的FSH-R mRNA表达无影响。因此，TGF-β对FSH-R mRNA和LH-R mRNA的影响可能是由于两种受体mRNA的稳定性增加和卵泡抑素的不敏感性。

项目成果

Minegishi T, et al.: "Molecular Cloning, Cellular Distribution and Regulation of Rat Steroidogenic Acute Regulatory Protein (StAR) in the Ovary"Journal of Reproduction and Development. 48(1). 1-15 (2002)

Minegishi T 等人：“大鼠卵巢中类固醇生成急性调节蛋白 (StAR) 的分子克隆、细胞分布和调节”生殖与发育杂志。

N.Kikuchi, et al.: "Changes in Leptin concentration during the estrus cycle and all days(24 hours) in BDF-I Female Adult Mice"日本受精着床学会雑誌. 19. 213-216 (2002)

N. Kikuchi 等人：“BDF-I 雌性成年小鼠发情周期和全天（24 小时）瘦素浓度的变化”日本受精与着床学会杂志 19. 213-216 (2002)。

Inoue K, et al.: "Effect of Transforming Growth Factor β on the Expression of Luteinizing Hormone Receptor in Cultured Rat Granulosa Cells"Biology of Reproduction. 67. 610-615 (2002)

Inoue K 等人：“转化生长因子 β 对培养的大鼠颗粒细胞中促黄体激素受体表达的影响”，《生殖生物学》67. 610-615 (2002)。

Yoshino M, et al.: "Early Growth Response Gene-1 Regulates the Expression of the Rat Luteinizing Hormone Receptor Gene"Biology of Reproduction. 66. 1813-1819 (2002)

Yoshino M 等人：“早期生长反应基因 1 调节大鼠黄体化激素受体基因的表达”生殖生物学。

T.Minegishi, et al.: "Molecular Cloning, Cellular Distribution and Regulation of Rat Steroidogenic Acute Regulatory Protein(StAR) in the Ovary"Journal of Reproduction and Development. 48(1). 1-15 (2002)

T.Minegishi 等人：“大鼠卵巢中类固醇生成急性调节蛋白 (StAR) 的分子克隆、细胞分布和调节”生殖与发育杂志。