Mechanisms of Regulation in Follicular Growth

卵泡生长的调节机制

• 批准号: 07044222
• 负责人: MINEGISHI Takashi
• 金额: $ 1.98万
• 依托单位: GUNMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044222/
• 关键词: LH; hCG receptor; splicimg; expression; co7 cell; cAMP; CHO cell; cAMP; カルシウム; 卵胞; アクチビン; FSHレセプター

Cloning of the human LH/HCG receptor cDNA previously revealed the existence of two alternative forms of the receptor differing by the presence (HLH-Ra) and absence (HLH-Rb) of 62 amino acids encoded by exon 9. We have studied functional significance of these receptor isoforms and have confirmed that they are generated by alternative splicing. A reverse transcription-polymerase chain reaction amplification was used to detect different isoforms of LH receptor mRNAs in ovary and placenta. The expression of the two mRNA forms of LH/HCG receptor were detected in ovary, and at very low concentrations in placenta. Treatment with HCG caused a dosedependent increase in cAMP production with an initial response evident at -1 ng/ml HCG in COS-7 cells expressing HLH-Ra. However, a complete loss of signal transduction was found in cells transfected with the truncated HLH-Rb.

人LH/HCG受体cDNA的克隆先前揭示了存在两种替代形式的受体，其不同之处在于外显子9编码的62个氨基酸的存在（HLH-Ra）和不存在（HLH-Rb）。我们已经研究了这些受体亚型的功能意义，并证实它们是由选择性剪接产生的。采用逆转录-聚合酶链反应扩增法检测卵巢和胎盘中LH受体mRNA的不同亚型。两种LH/HCG受体mRNA在卵巢中均有表达，在胎盘中表达量很低。在表达HLH-Ra的COS-7细胞中，用HCG处理引起cAMP产生的剂量依赖性增加，在-1 ng/ml HCG下的初始反应明显。然而，在用截短的HLH-Rb转染的细胞中发现信号转导完全丧失。

项目成果

T.Minegishi: "Gonadotrophin Receptors and Biassay for Human Serum Gonadotrophins(supplement)" J.et Assisted Reproduction and Genetics. 14.5. (1997)

T.Minegishi：“促性腺激素受体和人血清促性腺激素的偏差（补充）”J.et 辅助生殖和遗传学。

T. Minegishi: "Expression of follicle-stimulating hormone (FSH) receptor in human ovary" European J. of Clinical Investigation. 27・6. 469-474 (1997)

T. Minegishi：“人卵巢中促卵泡激素（FSH）受体的表达”欧洲临床研究杂志27・6（1997）。

中村学: "Interaction between activin A and cAMP in the induction of FSH receptor in cultured rat granulosa cells" Journal of Endocrinology. 147. 103-110 (1995)

Manabu Nakamura：“激活素 A 和 cAMP 在诱导培养的大鼠颗粒细胞中 FSH 受体的相互作用”内分泌学杂志 147. 103-110 (1995)。

伊藤郁朗: "視床下部性無月経のGnRHパルス療法" 臨床婦人科産科. 49. 300-302 (1995)

Ito, Ikuo：“GnRH 脉冲疗法治疗下丘脑闭经”《临床妇产科》49. 300-302 (1995)。

田野眞理: "Transcriptional and Rosttranscriptional Regulation of Follicle-stimulating Hormone Receptor in rat granulosa cells by 8-Br-3′,5′-Cyclic-Adenosine Monophoshate and Activin" Journal of Endocrinology. (1997)

Mari Tano：“8-Br-3,5-环磷酸腺苷和激活素对大鼠颗粒细胞中卵泡刺激激素受体的转录和 Rost 转录调节”内分泌学杂志（1997 年）。