Structure and function of gonadotropin receptors

促性腺激素受体的结构和功能

• 批准号: 10044235
• 负责人: MINEGISHI Takashi
• 金额: $ 1.73万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044235/
• 关键词: TSH Receptor; LH; hCG Receptor; FSH Receptor; Antibodies to receptors

Two different human LH receptor sequences have been published, differing by a six-base pair insertion encoding Leu-Gln at position 55-60. It has recently been proposed that this would reflect the existence of two LH receptor loci in the human genome. The present results demonstrate that both sequences exist as allelic variants in the Caucasian population. Allelic frequency of 'LQ variant' and 'wild-type' (αLQ) allele are 0.26 and 0.74 respectively. In contrast, the LQ allele is virtually absent from the Japanese population. Functional characterization of both alleles by transient expression in COS-7 cells did not reveal any difference between the two receptors, neither for cell surface expression nor for cAMP production and sensitivity to hCG/LH.

已经发表了两种不同的人LH受体序列，不同之处在于在位置55-60处插入编码Leu-Gln的六个碱基对。最近有人提出，这反映了人类基因组中存在两个LH受体基因座。目前的结果表明，这两个序列存在的等位基因变异的高加索人口。“LQ变异体”和“野生型”（αLQ）等位基因频率分别为0.26和0.74。相比之下，LQ等位基因在日本人群中几乎不存在。在COS-7细胞中瞬时表达的两个等位基因的功能表征没有发现两种受体之间的任何差异，无论是细胞表面表达，也不是cAMP的生产和敏感性hCG/LH。

项目成果

T.Minegishi: "Control of FSH receptor mRNA expression in rat granulosa cells by 3',5'-cyclic adenosine monophosphate, activin, and follistatin"Mol Cell Endocrinol. 149. 71-77 (1999)

T.Minegishi：“通过 3,5-环单磷酸腺苷、激活素和卵泡抑素控制大鼠颗粒细胞中 FSH 受体 mRNA 的表达”Mol Cell Endocrinol。

T.Hirakawa: "Effect of prolactin on the expression of luteinizing hormone receptors during cell differentiation in cultured rat granulosa cells"Endocrinology. 140・8. 3444-3451 (1999)

T. Hirakawa：“催乳素对培养的大鼠颗粒细胞分化过程中黄体生成素受体表达的影响”内分泌学140・8（1999）。

Y.Hosoi: "Stimulation of chinise hamster ovary cells expressing human thyrotropin receptors by serum human chorionic gonadotropin of patients with hydatidiform mole"Thyroid. 9・12. 1205-1210 (1999)

Y.Hosoi：“葡萄胎患者血清人绒毛膜促性腺激素对表达人促甲状腺素受体的中国仓鼠卵巢细胞的刺激”，甲状腺，1205-1210。

I.Ito: "Presence of activin signal transduction in normal ovarian cells and epithelial ovarian carcinoma"British J Cancer. (in press).

I.Ito：“正常卵巢细胞和上皮性卵巢癌中存在激活素信号转导”英国癌症杂志。

T.Hirakawa: "Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of FSH receptors during cell differentiation in cultured granulosa cells"Endocrinology. (in press).

T.Hirakawa：“2,3,7,8-四氯二苯并-对-二恶英 (TCDD) 对培养颗粒细胞分化过程中 FSH 受体表达的影响”内分泌学。