权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Structure and regulation of expression for LH/hCG receptor

LH/hCG 受体的结构和表达调控

基本信息

批准号：
02670732
负责人：
MINEGISHI Takashi
金额：
$ 1.41万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670732/
关键词：
Gonadotropin Receptor Molecular biology ゴナドトロビン

项目摘要

The regulation of testicular and ovarian function by gonadotopic hormone is is mediated by specific, high-affinity receptors that are located in the plasma membrane of the respective target cells. The immediate response of target cells to the binding of these hormones are an increase in adenyl cyclase mediated by G protein. The maturation of ovarian follicles and their transformation to corpus luteum is regulated by a specific set of hormones and has been a major research area for this project.After follicle maturation, administration of hCG induce its luteinization. The appearance of corpus lutea was accompanied by increase of hCG binding. Our studies demonstrate that the effect of PMSG and hCG on ovarian gonadotropin receptors are closely related to the action of these hormones on gonadotropin receptor mRNA levels. These studies again demonstrated hCG-induced decline in LH/HCG receptor binding was associated with hCG induced down regulation of message levels.On the other hand, We have isolated and sequenced cDNA encoding the human gonadotropin receptors. Oligo-primed cDNA were prepared from human ovary polyadenylated RNAs. The library was screened with ^<32>P-labeled 2.5Kb and 2.0Kb fragment from rat LH/hCG and FSH receptor cDEIA clones. A putative extradomain of 335 and 359 amino acid precedes a region of seven transmembrane segments for the human LH/hCG receptor and FSH receptor, respectively. Mature protein should consist of 674 and 695 amino acids(75632 and 76464 daltons), and six and four potential N-linked glycosylation sites were found in the putative extracellular domain for the LH/hCG and FSH receptor.Based upon these finding, we are going to study more detail relationship between structure and function of gonadotropin receptors.

性腺激素对睾丸和卵巢功能的调节是由位于相应靶细胞质膜上的高亲和力受体介导的。靶细胞对这些激素结合的直接反应是G蛋白介导的腺苷环化酶增加。卵泡的成熟及其向黄体的转化受一组特定激素的调节，一直是该项目的主要研究领域。卵泡成熟后，给予hCG诱导其黄体化。黄体的出现伴随着hCG结合量的增加。我们的研究表明，PMSG和hCG对卵巢促性腺激素受体的影响与这些激素对促性腺激素受体mRNA水平的作用密切相关。这些研究再次证明了hCG诱导的促黄体生成素/人绒毛膜促性腺激素受体结合的下降与hCG诱导的信息水平下调有关。另一方面，我们已经分离并测序了人促性腺激素受体的编码基因。以人卵巢多聚腺苷RNA为模板，制备寡核苷酸引发的c DNA。用大鼠黄体生成素/人绒毛膜促性腺激素和促性腺激素受体基因克隆的2.5Kb和2.0Kb片段筛选文库。人促黄体生成素/人绒毛膜促性腺激素受体和卵泡刺激素受体的外区分别有335和359个氨基酸，位于7个跨膜区段的前面。成熟的蛋白质由674和695个氨基酸组成(75632和76464道尔顿)，在黄体生成素/人绒毛膜促性腺激素和卵泡刺激素受体的胞外区发现了6个和4个潜在的N-连接糖基化位点。