Development of treatment method to inhibit progress of root surface caries-Evaluation of the effects of impregnation of antibacterial resin

本课题通过体外实验研究了含抗菌单体MDPB（12-甲基丙烯酰氧十二烷基溴化吡啶）的牙本质结合体系（DBS）对牙根表面龋病进展的抑制作用。经琼脂盘扩散法和MIC/MBC测定，各专利DBS对口腔细菌均无明显抑菌作用。2 .含MDPB的抗菌引物杀菌活性高于其他市售产品，使用含4% MDPB的引物可完全杀灭脱矿牙本质中的细菌。含mdpb的引物能够深入穿透人工和自然的龋损。酸凝胶或变形链球菌培养技术产生的人工牙根龋齿病变的进展完全被含有mdpb的dbs的应用所阻止。应用含mdpb的DBS可抑制牙本质表面菌斑的积累，应用含mdpb的结合树脂可观察到较强的抑制作用。这些结果表明，含mdpb的DBS可有效抑制根表面龋的进展，并可能有益于治疗活动性根龋病变。

In this research project, the effects of application of a dentin bonding system (DBS) containing the antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) on inhibition of progress of root surface caries were examined by in vitro studies.1.None of the proprietary DBS demonstrated significant antibacterial effects against oral bacteria when tested by agar-disc diffusion method and MIC/MBC determination.2.Bactericidal activities of antibacterial primers containing MDPB were greater than those of any other commercial products, and the application of 4% MDPB-containing primer resulted in complete killing of bacteria in demineralized dentine.3.MDPB-containing primers were capable of penetrating deeply into the artificial and natural carious lesions.4.The progression of artificial root carious lesions, produced by either an acid-gel or a Streptococcus mutans culture technique, was completely prevented by the application of MDPB-containing DBS.5.Plaque accumulation on the surface of the demineralized dentin was inhibited when MDPB-containing DBS was applied, strong inhibition being observed by the application of MDPB-containing bonding resin.These results indicate that MDPB-containing DBS is effective in inhibiting the progress of root surface caries and of possible benefit for managing active root carious lesions.