In vivo efficacy of an adhesive system with a self-etching primer incorporating the antibacterial monomer MDPB

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In this study, clinical benefit of a newly developed dentin bonding system with a self-etching primer incorporating the antibacterial monomer MDPB(12-methacryloyloxydodecylpyridinium bromide)at 5% were investigated, mainly by in vivo tests using beagle dogs.1. When the killing activities against the bacteria recovered from human carious dentin were investigated, the experimental primer was more bactericidal compared with proprietary prirners.2. The model system simulating the cavity which contain residual bacteria was established by inoculating Streptococcus mutans to the cavity prepared in the tooth of a dog. The dentinal sample was collected after application of the experimental primer or parent primer to the cavity, and the number of viable bacteria was determnined. No viable bacteria were recovered after application of the experimental primer, and the bacterial recovery was signiflcantly greater for the parent primer group. The cavities inoculated with S.mutans were also restored with resin composite using the experimental or parent primer, and the pulp response was histopathologically examined after 7 or 30 days. The teeth restored with the experimental primer showed only slight odontoblastic changes at 7-day evaluation period, although mild infiltration of inflammatory cells was observed for restorations with the parent primer.3. After 7 days, the teeth restored with the experimental primer showed no inflammatory responses, and bonding interfaces with 1 μm-thick hybrid layer and resin tag fomation in vivo.These results indicate that the experimental primer containing MDPB exhibited antibacterial effects, biocompatibility, and reliable bonding abilities not only in vitro but also in vivo, possibly being beneficial for clinical use.