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In vivo efficacy of an adhesive system with a self-etching primer incorporating the antibacterial monomer MDPB

含有抗菌单体 MDPB 的自蚀刻底漆粘合系统的体内功效

基本信息

批准号：
10557177
负责人：
IMAZATO Satoshi
金额：
$ 8.64万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557177/
关键词：
Dentin bonding system Antibacterial activity In vivo Dentin Primer Pulpal reaction Biocompatibility Streptococcus mutans Self-etching primer 殺菌効果

项目摘要

In this study, clinical benefit of a newly developed dentin bonding system with a self-etching primer incorporating the antibacterial monomer MDPB(12-methacryloyloxydodecylpyridinium bromide)at 5% were investigated, mainly by in vivo tests using beagle dogs.1. When the killing activities against the bacteria recovered from human carious dentin were investigated, the experimental primer was more bactericidal compared with proprietary prirners.2. The model system simulating the cavity which contain residual bacteria was established by inoculating Streptococcus mutans to the cavity prepared in the tooth of a dog. The dentinal sample was collected after application of the experimental primer or parent primer to the cavity, and the number of viable bacteria was determnined. No viable bacteria were recovered after application of the experimental primer, and the bacterial recovery was signiflcantly greater for the parent primer group. The cavities inoculated with S.mutans were also restored with resin composite using the experimental or parent primer, and the pulp response was histopathologically examined after 7 or 30 days. The teeth restored with the experimental primer showed only slight odontoblastic changes at 7-day evaluation period, although mild infiltration of inflammatory cells was observed for restorations with the parent primer.3. After 7 days, the teeth restored with the experimental primer showed no inflammatory responses, and bonding interfaces with 1 μm-thick hybrid layer and resin tag fomation in vivo.These results indicate that the experimental primer containing MDPB exhibited antibacterial effects, biocompatibility, and reliable bonding abilities not only in vitro but also in vivo, possibly being beneficial for clinical use.

在这项研究中，主要通过在Beagle狗身上进行体内试验，研究了一种新开发的牙本质粘结系统的临床益处，该粘接系统使用自酸蚀底漆，其中加入了5%的抗菌单体MDPB(12-甲基丙烯酰氧基十二烷基溴化吡啶)。当对从人龋牙本质中回收的细菌的杀灭活性进行检测时，实验底物比专有底物具有更强的杀菌活性。将变形链球菌接种到狗牙齿的空洞中，建立了模拟含有残留细菌的空洞的模型系统。将实验底漆或亲本底漆涂抹到洞内后，采集牙本质标本，测定活菌数。使用该实验引发剂后，未回收到活细菌，且亲本引物组的细菌回收率明显高于亲本引发剂组。接种变形链球菌的空洞也用实验或亲本底漆用树脂复合体修复，7天或30天后进行牙髓组织病理学检查。实验用底漆修复的牙齿在7天的评估期内仅有轻微的成牙本质细胞变化，而用亲本底漆修复的牙齿则有轻微的炎症细胞渗入。7天后，用该实验底漆修复的牙齿无炎症反应，与1μm厚的杂交层和树脂粘接界面在体内形成，表明含有MDPB的实验底漆无论在体外还是在体内都具有良好的抗菌效果、生物相容性和可靠的粘接能力，可能有利于临床应用。