Influence of incorporation of antibacterial monomer MDPB on various properties of adhesive system

在这项研究中，研究了新开发的含有抗菌单体MDPB（12-甲基丙烯酰氧十二烷基溴化吡啶）的粘接体系的体外抗菌效果、结合强度和细胞毒性。将含有1、2或5% MDPB的实验引物和含有2.5% MDPB的粘合剂加入到专有的自蚀刻/底漆系统中。FTIR对底漆/胶粘剂复合物转化度的测量表明，任何实验材料与不含MDPB的对照之间均无显著差异。与对照相比，未固化的实验引物和黏合剂对口腔变形链球菌等细菌的生长有更大的抑制作用，其中含有5% MDPB的引物在短时间内对变形链球菌的杀灭效果最好。此外，含mdpb的引物和胶粘剂即使在固化后也能抑制变形链球菌在其表面的生长。含MDPB的引物和胶粘剂与牙本质的拉伸结合强度均未降低。相反，含有1%和2% MDPB的引物比其他引物（包括对照）具有更高的结合强度。利用人牙髓细胞进行的牙本质屏障试验表明，在引物中掺入高达5%的MDPB对细胞毒性没有显著影响。结果表明，掺入MDPB可以在不影响结合强度和细胞毒性的情况下，在固化前后形成具有抗菌活性的牙本质粘接体系，提示含有MDPB的牙本质粘接体系具有临床应用的可能性。

In this study, the antibacterial effect, bond strength, and cytotoxicity of newly developed adhesive systems incorporating the antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) were investigated in vitro.Experimental primers containing 1, 2, or 5% MDPB, and adhesive with 2.5% MDPB were prepared by the addition of MDPB into the components of proprietary self-etching/priming system.The measurement of degree of conversion of the primer/adhesive complex by FTIR indicated no significant differences between any of the experimental materials and the control without MDPB.Uncured experimental primers and adhesive showed greater inhibitory effect on the growth of oral bacteria such as Streptococcus mutans compared with control, and the primer containing 5% MDPB was most effective to kill S.mutans within a short period.Additionally, MDPB-containing primer and adhesive exhibited an inhibitory effect on the growth of S.mutans on its surface even after being cured.No decrease in tensile bond strength to dentin was observed for both primers and adhesive containing MDPB.On the contrary, the primer incorporating 1 and 2% MDPB showed higher bond strength than all the others, including the control.By dentine barrier tests using human pulpal cells, incorporation of MDPB into the primer of up to 5% has been demonstrated to have no significant influence on the cytotoxicity.The results indicate that providing a dentin bonding system with antibacterial activity before and after curing could be achieved by incorporation of MDPB without influencing bond strength or cytotoxicity, suggesting that the possibility of MDPB-containing adhesive system for clinical use.