Influence of incorporation of antibacterial monomer MDPB on various properties of adhesive system

在这项研究中，新开发的粘合剂系统的抗菌效果，粘结强度和细胞毒性，包括抗菌单体MDPB（12-甲基丙烯酰氧基十二烷基溴化吡啶）进行了体外研究。实验引物含有1，2，或5%MDPB，通过将MDPB添加到自酸蚀/热处理专用胶的组分中，制备了含2.5% MDPB的胶粘剂和含2.5% MDPB的胶粘剂。通过FTIR测量底漆/粘合剂复合物的转化程度表明，任何实验材料与不含MDPB的对照之间没有显著差异。与对照相比，未固化的实验底漆和粘合剂对口腔细菌如变形链球菌的生长显示出更大的抑制作用，含5%MDPB的底涂剂和粘接剂在短期内对变形链球菌的杀灭效果最好，即使在固化后，底涂剂和粘接剂对变形链球菌的生长也有抑制作用，对牙本质的抗张粘接强度没有降低，相反，含MDPB的底涂剂和粘接剂对牙本质的抗张粘接强度没有降低。加入1%和2% MDPB的底漆显示出比包括对照在内的所有其它底漆更高的粘结强度。通过使用人牙髓细胞的牙本质屏障测试，将MDPB掺入引物中，最多5%结果表明，提供一种牙本质粘结系统，MDPB的加入可使粘接剂在固化前后均具有抗菌活性，且不影响粘接强度和细胞毒性，提示含MDPB的粘接剂体系具有临床应用的可能性。

In this study, the antibacterial effect, bond strength, and cytotoxicity of newly developed adhesive systems incorporating the antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) were investigated in vitro.Experimental primers containing 1, 2, or 5% MDPB, and adhesive with 2.5% MDPB were prepared by the addition of MDPB into the components of proprietary self-etching/priming system.The measurement of degree of conversion of the primer/adhesive complex by FTIR indicated no significant differences between any of the experimental materials and the control without MDPB.Uncured experimental primers and adhesive showed greater inhibitory effect on the growth of oral bacteria such as Streptococcus mutans compared with control, and the primer containing 5% MDPB was most effective to kill S.mutans within a short period.Additionally, MDPB-containing primer and adhesive exhibited an inhibitory effect on the growth of S.mutans on its surface even after being cured.No decrease in tensile bond strength to dentin was observed for both primers and adhesive containing MDPB.On the contrary, the primer incorporating 1 and 2% MDPB showed higher bond strength than all the others, including the control.By dentine barrier tests using human pulpal cells, incorporation of MDPB into the primer of up to 5% has been demonstrated to have no significant influence on the cytotoxicity.The results indicate that providing a dentin bonding system with antibacterial activity before and after curing could be achieved by incorporation of MDPB without influencing bond strength or cytotoxicity, suggesting that the possibility of MDPB-containing adhesive system for clinical use.