Human characteristic mechanism of enzyme induction and the prediction

作为一种评估人CYP3A4诱导的工具，我们已经成功地制备了包括CYP3A4启动子和增强子区域的腺病毒载体系统。利用该载体在HepG2细胞中获得稳定活性的克隆。在选择的克隆中，对克隆响应于典型的化学诱导，并显示高荧光素酶报告活性。这些克隆被认为是候选人作为有效的工具，定量预测诱导潜力的人CYP3A4。我们目前正在评估克隆的实用工具。

As an estimation tool for human CYP3A4 induction, we have succeeded to make an adenovirus vecter system including CYP3A4 promoter and enhancer regions. The vecter was used to obtain the stably active clones in HepG2 cells. Among the clones selected, couple clones responded to typical chemical induces and showed high luciferase reporter activities. These clones are found to be candidates as effective tools for the quantitative prediction of inducing potential for human CYP3A4. We are currently evaluating the clones for the practical tools.