Analysis of mechanism of neonatal imprinting of drug-metabolizing enzymes of rat liver

大鼠肝脏药物代谢酶新生期印记机制分析

• 批准号: 60480130
• 负责人: YAMAZOE Yasushi
• 金额: $ 4.22万
• 依托单位: School of Medicine, Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480130/
• 关键词: sex-specific cytochrome P-450; androgen; estrogen; growth hormone; steroid hormone hydroxylases; 薬物代謝酵素

The effects of neonatal imprinting on sex-specific form of cytochrome P-450, P-450-male and P-450-female in rat hepatic microsomes were studied in comparison with the effects on testosterone hydroxylations and drug oxidations. The rats castrated on one day completely lost the expression of P-450-male at 8 weeks of age, but the rats castrated on 5 days had half content of P-450-male of normal adult rats.However, the administration of testosterone at 8 weeks of age, completely reversed the effect of neonatal castration, in contrast to previous works on testosterone hydroxylations.The administration of sodium glutamate to neonatal male rats changes the pattern of P-450-male, testosterone hydroxylation and drug oxidation from the male pattern to the female.

本文研究了新生印迹对大鼠肝微粒体细胞色素P-450性别特异性形式的影响，并与对睾酮羟化和药物氧化的影响进行了比较。去势1天的大鼠在8周龄时完全丧失P-450-male的表达，而去势5天的大鼠P-450-male的含量只有正常成年大鼠的一半，但8周龄时给予睾酮，完全逆转了新生儿去势的作用，与先前关于睾酮羟化的工作相反，对新生雄性大鼠施用谷氨酸钠改变了P-450-雄性的模式，睾酮羟化和药物氧化从男性模式到女性。

项目成果

Yamazoe, Y., Shimada, M., Kamataki, T. and Kato, R.: "Effects of hypophysectomy and growth hormone treatment on sex-specific forms of cytochrome P-450 in relation to drug and steroid metabolisms in rat liver microsomes." Japan. J. Pharmacol.42. 371-382 (1

Yamazoe, Y.、Shimada, M.、Kamataki, T. 和 Kato, R.：“垂体切除术和生长激素治疗对细胞色素 P-450 性别特异性形式的影响，与大鼠肝微粒体中药物和类固醇代谢有关。

加藤隆一,山添康: 医学のあゆみ. (1987)

加藤龙一、山添靖：医学史（1987）。

Yamazoe, Y., Shimada, M., Murayama, N., Kawano, S. and Kato, R.: "The regulation by growth hormone of microsomal testosterone 6 <beta> -hydroxylase in male rat livers." J. Biochem.100. 1095-1097 (1986)

Yamazoe, Y.、Shimada, M.、Murayama, N.、Kawano, S. 和 Kato, R.：“生长激素对雄性大鼠肝脏中微粒体睾酮 6 β-羟化酶的调节。”

Yamazoe,Y;Shimada,M;Kamataki,T;Kato,R.: Japan.J.Pharmacol.42. 371-382 (1986)

Yamazoe，Y；Shimada，M；Kamataki，T；Kato，R.：Japan.J.Pharmacol.42。

Kato,R;Yamazoe,Y;Shimada,M;Murayama,N;Kamataki,T.: J.Biochem.100. 895-902 (1986)

加藤，R；山添，Y；岛田，M；村山，N；镰泷，T.：J.Biochem.100。