Analysis of mechanism of neonatal imprinting of drug-metabolizing enzymes of rat liver

研究了新生儿印迹对大鼠肝微粒体细胞色素P-450、P-450-雄性和P-450-雌性的影响，并比较了其对睾酮羟基化和药物氧化的影响。1天阉割的大鼠在8周龄时P-450-male完全丧失表达，而5天阉割的大鼠P-450-male含量为正常成年大鼠的一半。然而，在8周龄时给予睾酮，完全逆转了新生儿阉割的影响，这与之前对睾酮羟基化的研究相反。谷氨酸钠可改变新生雄性大鼠p -450-雄性、睾酮羟基化和药物氧化的模式，使其从雄性模式转变为雌性模式。

The effects of neonatal imprinting on sex-specific form of cytochrome P-450, P-450-male and P-450-female in rat hepatic microsomes were studied in comparison with the effects on testosterone hydroxylations and drug oxidations. The rats castrated on one day completely lost the expression of P-450-male at 8 weeks of age, but the rats castrated on 5 days had half content of P-450-male of normal adult rats.However, the administration of testosterone at 8 weeks of age, completely reversed the effect of neonatal castration, in contrast to previous works on testosterone hydroxylations.The administration of sodium glutamate to neonatal male rats changes the pattern of P-450-male, testosterone hydroxylation and drug oxidation from the male pattern to the female.