New Strategy for Periodontal Treatment Based on Control of Angiogenesis in Periodontal Tissues using VEGF and Anti-VEGF.

基于使用 VEGF 和抗 VEGF 控制牙周组织血管生成的牙周治疗新策略。

• 批准号: 13557191
• 负责人: IZUMI Yuichi
• 金额: $ 8.45万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557191/
• 关键词: Periodontal disease; Vascular endothelial growth factor; Gingival fibroblasts; Periodontal ligament fibroblasts; Periodontal pathogens; Gingival crevicular fluid; Periodontal regeneration; ポリ乳酸; マイクロカプセル; 歯周組織再生; 歯周病関連細菌

Vascular endothelial growth factor (VEGF) has recently attracted attention as a potent inducer of vascular permeability and angiogenesis. To clarify the role of VEGF in periodontal lesion, we investigated 1) the-properties of VEGF expression in the cells derived from human periodontal tissues in culture, 2) the detection of the changing levels of VEGF in gingival crevicular fluid (GCF) following two months of scaling and root planning (SRP), and 3) the effects of VEGF on human periodontal ligament fibroblasts (HPLF) culture through the induction of osteogenic markers and nodule formation. The results showed that 1) human gingival fibroblasts (HGF) constitutively produced VEGF and levels were significantly enhanced by stimulation with Ve and OMP from A.actinomycetemcomitans and P.gingivalis. Supernatant from cultures of Ve-and OMP-stimulated HGF strongly induced vascular permeability enhancement, and 2) that total amounts of VEGF in GCF might indicate the severity of periodontitis and t … More he concentration of VEGF could predict the healing response of periodontal tissues following SRP. These results suggest that VEGF may be associated with the etiology of periodontitis in its early stages according to neovascularization stimulated by periodontal pathogens causing swelling and edema. Furthermore, the results revealed 3) that VEGF enhanced the expression of c-fos, osteopontin, osteocalcin, bone morphogenesis protein-2 and periodontal ligament associated protein-1 in HPLF culture by stimulation with VEGF^<121> and VEGF^<165>, but not in HGF. VEGF^<165> induced these osteogenic markers more strongly than VEGF^<121>. The alkaline phosphatase activity and calcium concentration were up-regulated after HPLF culture was stimulated with VEGF^<121> and VEGF^<165> for 15 days, while nodule formations also up-regulated after 21 days. These results suggest that HPLF has a phenotype similar to osteoblasts after being stimulated with VEGF, and VEGF may have an important role in initiating the process of periodontal tissue healing. Less

血管内皮生长因子（VEGF）作为一种强有力的血管通透性和血管生成诱导剂，近年来引起了人们的关注。为探讨VEGF在牙周组织损伤中的作用，本研究采用体外培养的人牙周组织来源的细胞，观察VEGF表达的特点，检测龈沟液（GCF）中VEGF水平在洁治和根面平整术（SRP）后两个月的变化，3）VEGF对人牙周膜成纤维细胞（human periodontal ligament fibroblasts，HPLF）成骨标志物和结节形成的影响。结果表明：1）人牙龈成纤维细胞（HGF）组成性产生VEGF，伴放线菌和牙龈卟啉单胞菌的Ve和OMP可显著增强HGF的表达; Ve和OMP刺激的HGF培养上清强烈诱导血管通透性增加，2）GCF中VEGF的总量可能指示牙周炎的严重程度， ...更多信息 VEGF浓度可预测SRP后牙周组织的愈合反应。这些结果表明，VEGF可能与牙周炎的病因在其早期阶段，根据牙周病原体引起肿胀和水肿刺激新生血管。3）VEGF可促进HPLF中c-fos、骨桥蛋白、骨钙素、骨形态发生蛋白-2和牙周膜相关蛋白-1的表达<121><165>，而对HGF无明显影响。VEGF-C<165>比VEGF-C更强烈地诱导这些成骨标志物<121>。在用VEGF-A和VEGF-B刺激HPLF培养物15天后，碱性磷酸酶活性和钙浓度上调<121><165>，而结节形成也在21天后上调。这些结果表明，HPLF在VEGF刺激后具有类似于成骨细胞的表型，VEGF可能在启动牙周组织愈合过程中起重要作用。少

项目成果

Kanyarat Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"Journal of Periodontal Research. 38・1. 90-96 (2003)

Kanyarat Suthin：“牙龈成纤维细胞中牙周病原体增强血管内皮生长因子的表达”《牙周研究杂志》38・1（2003）。

Kanyarat Suthin: "Possible roles of VEGF in periodontal tissue : Expression of VEGF in human gingiva and periodontal ligament fibroblasts and its effects on cells in culture"Journal of Hard Tissue Biology. 10・2. 85-88 (2001)

Kanyarat Suthin：“VEGF 在牙周组织中的可能作用：VEGF 在人牙龈和牙周膜成纤维细胞中的表达及其对培养细胞的影响”硬组织生物学杂志 10・2 (2001)。

Kanyarat Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"Journal of Periodontal Research. (in press).

Kanyarat Suthin：“牙龈成纤维细胞中牙周病原体增强血管内皮生长因子的表达”牙周研究杂志。

石川久美子: "歯周病患者歯肉溝滲出液中に検出されるvascular endothelial growth factor (VEGF)とその変動"日歯周誌. 44. 387-395 (2002)

Kumiko Ishikawa：“牙周病患者龈沟液中检测到的血管内皮生长因子（VEGF）及其波动”日本牙周杂志 44. 387-395 (2002)。

石川 久美子: "歯周病患者歯肉溝滲出液中に検出されるvascular endothelial growth factor(VEGF)とその変動"日本歯周病学会会誌. 44・4. 387-395 (2002)

石川久美子：“牙周病患者龈沟液中的血管内皮生长因子（VEGF）及其波动”日本牙周病学会杂志44・4（2002）。